&lt;p&gt;Stability and osteogenic potential evaluation of micro-patterned titania mesoporous-nanotube structures&lt;/p&gt;

Ding, Xi; Wang, Yuzhen; Xu, Lihua; Zhang, Hualin; Deng, Zhennan; Cai, Leyi; Wu, Zuosu; Yao, Litao; Wu, Xinghai; Liu, Jinsong; Shen, Xinkun

doi:10.2147/ijn.s199610

Cited by 10 publications

(5 citation statements)

References 46 publications

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“…Specifically, both nanotubular surfaces exhibited higher levels of ALP activity in comparison with the flat Ti surface. This finding is not surprising since, overall, nanotubular TiO 2 surfaces are well known for their ability to enhance ALP activity [27,31,74]. However, in the present study, this enhancement was significant in the case of the pre-osteoblasts grown on TNT80 substrate.…”

Section: Resultscontrasting

confidence: 45%

Lateral Spacing of TiO2 Nanotubes Modulates Osteoblast Behavior

et al. 2019

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Titanium dioxide (TiO2) nanotube coated substrates have revolutionized the concept of implant in a number of ways, being endowed with superior osseointegration properties and local drug delivery capacity. While accumulating reports describe the influence of nanotube diameter on cell behavior, little is known about the effects of nanotube lateral spacing on cells involved in bone regeneration. In this context, in the present study the MC3T3-E1 murine pre-osteoblast cells behavior has been investigated by using TiO2 nanotubes of ~78 nm diameter and lateral spacing of 18 nm and 80 nm, respectively. Both nanostructured surfaces supported cell viability and proliferation in approximately equal extent. However, obvious differences in the cell spreading areas, morphologies, the organization of the actin cytoskeleton and the pattern of the focal adhesions were noticed. Furthermore, investigation of the pre-osteoblast differentiation potential indicated a higher capacity of larger spacing nanostructure to enhance the expression of the alkaline phosphatase, osteopontin and osteocalcin osteoblast specific markers inducing osteogenic differentiation. These findings provide the proof that lateral spacing of the TiO2 nanotube coated titanium (Ti) surfaces has to be considered in designing bone implants with improved biological performance.

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Section: Resultscontrasting

confidence: 45%

Lateral Spacing of TiO2 Nanotubes Modulates Osteoblast Behavior

et al. 2019

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“…The cell morphology experiment was conducted by staining at precise time points according to previous studies. 28 Cell morphology on different samples were confirmed by fluorescence microscopy (FM) and FE-SEMS-4800 (Hitachi). As for fluorescence microcopy observation, cells on samples were fixed by 4% paraformaldehyde (Sigma, USA) for an hour after three days of cell culture and then stained by phalloidin (Beyotime, China) for an hour and DAPI (Beyotime, China) for 15 min respectively.…”

Section: Cell Morphologymentioning

confidence: 99%

<p>The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant</p>

Wang

et al. 2020

IJN

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“…Thus, the Ta coating played a more important role in promoting osteogenic properties, and the FHA coating had a significant role in enhancing cytocompatibility. In addition, studies have proven that the nanostructure surface also plays an important role in osteogenic promotion ( Ding et al, 2019 ; Lee et al, 2015 ). Thus, the combination of the FHA and Ta coatings could synergistically enhance the biological property of the samples.…”

Section: Resultsmentioning

confidence: 99%

Osteoinduction Evaluation of Fluorinated Hydroxyapatite and Tantalum Composite Coatings on Magnesium Alloys

Cao

Yang

et al. 2021

Front. Chem.

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Magnesium (Mg) alloys have a wide range of biomaterial applications, but their lack of biocompatibility and osteoinduction property impedes osteointegration. In order to enhance the bioactivity of Mg alloy, a composite coating of fluorinated hydroxyapatite (FHA) and tantalum (Ta) was first developed on the surface of the alloy through thermal synthesis and magnetron sputtering technologies in this study. The samples were characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), energy dispersive spectroscopy (EDS) mapping, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and water contact angle measurement (WCA), which characterized the surface alternation and confirmed the deposition of the target FHA/Ta coating. The results of cell morphology showed that the MC3T3-E1 cells on the surface of Mg/FHA/Ta samples had the largest spreading area and lamellipodia. Moreover, the FHA coating endowed the surface with superior cell viability and osteogenic properties, while Ta coating played a more important role in osteogenic differentiation. Therefore, the combination of FHA and Ta coatings could synergistically promote biological functions, thus providing a novel strategy for implant design.

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<p>Stability and osteogenic potential evaluation of micro-patterned titania mesoporous-nanotube structures</p>

Cited by 10 publications

References 46 publications

Lateral Spacing of TiO2 Nanotubes Modulates Osteoblast Behavior

Lateral Spacing of TiO2 Nanotubes Modulates Osteoblast Behavior

<p>The Fabrication and Function of Strontium-modified Hierarchical Micro/Nano Titanium Implant</p>

Osteoinduction Evaluation of Fluorinated Hydroxyapatite and Tantalum Composite Coatings on Magnesium Alloys

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