<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

Abstract

Background: Glioma is a common malignant tumor worldwide. Sevoflurane (Sev) has been reported to inhibit the metastasis of glioma cells, but the underlying molecular mechanism needs further exploration. Methods: Cell Counting Kit-8 (CCK8) assay was used to check cell viability. Flow cytometry assay was hired to check cell apoptosis. The protein levels of B-cell lymphoma-2 (Bcl-2), BCL2-Associated X (Bax), hexokinase 2 (HK2) and magnesium transporter 1 (MAGT1) in samples were measured by Western blot. The abilities of cell migration and invasion were estimated by transwell assay. Glucose colorimetric assay kit and lactate colorimetric assay kit were used to check glucose consumption and lactate production, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the levels of circular RNA (circRNA) circ_0002755 (also known as the circRNA1656) and microRNA (miR)-628-5p in samples. The interaction between miR-628-5p and circ_0002755 or MAGT1 was predicated by starBase, which was verified by the dual-luciferase reporter assay. Xenograft tumor model was established to explore the biological role of circ_0002755 in vivo. Results: Sev inhibited cell viability, migration, invasion and promoted cell apoptosis, and also reduced glucose consumption and lactate production. Circ_0002755 was significantly upregulated in glioma tissues and cells, while its level was notably declined under Sev treatment. Besides, overexpression of circ_0002755 overturned Sev-mediated inhibitory effect on glioma progression. Further research indicated that circ_0002755 targeted miR-628-5p, and miR-628-5p targeted MAGT1, and Sev modulated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis. Moreover, Sev hindered tumor growth in vivo. Conclusion: Sev mediated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis.

Sevoflurane blocks glioma malignant development by upregulating circRELN through circRELN-mediated miR-1290/RORA axis

“…Sev was documented to inhibit glioma cell proliferation and invasion and induce apoptosis by degrading multiple oncogenes [ 23 , 24 ]. Besides, recent studies introduced that Sev blocked the progression of glioma by targeting the circRNA regulatory networks [ 11 , 25 ], suggesting that Sev had pivotal roles in mediating the deregulation of circRNAs. In our study, we found that circRELN expression, decreased in glioma tissues and cells, was inversely enhanced in Sev-treated glioma cells.…”

Tip of the Iceberg: Roles of CircRNAs in Cancer Glycolysis

“…Sevoflurane reduces the consumption of glucose and the production of lactic acid through the signal axis, thus inhibiting tumor growth, migration as well as promoting cell apoptosis in the body. 112 Besides, lidocaine inhibits the aerobic glycolysis of CRC cells through the circHOMER1/miR-138-5p/HEY1 axis, thereby promoting cell apoptosis in vitro and inhibiting tumor growth in vivo. 113 These reports have laid the foundation for targeted cancer therapy, but the regulatory network of circRNA and glucose metabolism still needs to be further enriched in the years ahead to obtain effective targeted therapy methods for malignant tumors.…”

Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p

“…A number of studies have shown that circRNAs can regulate the progress of glioma by adsorbing miRNAs, which called competing endogenous RNAs (ceRNA) mechanism [18,19]. An online database (circInteractome, https://circinteractome.nia.nih.…”