Hsa_circ_0091581 promotes glioma progression by regulating RMI1 via sponging miR-1243-5p

Abstract

Glioma is a pervasive malignancy and the main cause of cancer-related deaths worldwide. Circular RNA is an important subject of cancer research, and its role and function in glioma are poorly understood. This study demonstrated that hsa_circ_0091581 is upregulated in glioma tissues and cells. The results of the CCK-8, EdU, and transwell assays indicated that hsa_circ_0091581 promotes proliferation, migration, and invasion of glioma cells. The results of the luciferase reporter and RNA immunoprecipitation assays indicated that the mechanism of the effects of hsa_circ_0091581 on glioma cells involves sponging miR-1243-5p to regulate RMI1. The results of the rescue experiments indicated that hsa_circ_0091581 regulates proliferation, migration, and invasion of glioma cells by targeting RMI1 in a miR-1243-5p dependent manner. The results of the nude mice xenograft assays showed that knockdown of hsa_circ_0091581 inhibits glioma growth in vivo. Thus, our study determined the role of hsa_circ_0091581/miR-1243-5p/RMI1 in glioma and suggests that this axis may be a novel therapeutic target in glioma.

Profiling Secreted miRNA Biomarkers of Chemical-Induced Neurodegeneration in Human iPSC-Derived Neurons

“…Two upregulated miRNAs, miR-1305, and -1243, have been less extensively investigated, with no prior evidence elucidating their roles in neurological processes or dysfunctions, but the expression of these miRNAs have been confirmed in human brain ( Hiramoto et al , 2017 ; Ludwig et al , 2016 ; Qian et al , 2021 ; Su et al , 2020 ; Zhang et al , 2017 ). These miRNAs were of particular interest, because they were highly upregulated across multiple chemicals, and shown to target mRNA molecules related to TGF-β signaling (TGF-β2, SMAD2, or SMAD4).…”

The regulatory role and clinical application prospects of circRNA in the occurrence and development of CNS tumors

“… 121 circ‐0001801, hsa_circ_0043278, hsa_circ_0006168, circ_0001588, circMELK, circSERPINE2, circLGMN, and circ‐PITX1 were significantly up‐regulated in glioblastoma (GBM) and accelerated the glioma tumorigenesis via miR‐628‐5p/HMGB3, 122 miRNA‐638/HOXA9, 123 miR‐628‐5p/IGF1R, 124 miR‐211‐5p/YY1, and miR‐1281/ERBB4, 125 , 126 miR‐593/EphB2, 127 miR‐361‐3p/miR‐324‐5p/BCL2, 128 miR‐127‐3p/LGMN, 129 miR‐379‐5p/MAP3K2 130 axis. Multiple circRNA were identified that have potential as a therapeutic target in glioma, such as circPITX1, 131 circ_101064, 132 circ‐ATXN1, 133 circPARP4, 134 circHECTD1, 135 hsa_circ_0091581, 136 circZNF609, 137 , 138 circ_0001367, 139 , 140 circ_CLIP2, 141 circRNA‐0002109, 142 circPOSTN, 143 circCDK14, 144 and circBFAR. 145 Modulating the expression of these circRNAs may inhibit glioma development.…”