<p>Selenium Nanoparticles Synthesized Using <em>Pseudomonas stutzeri</em> (MH191156) Show Antiproliferative and Anti-angiogenic Activity Against Cervical Cancer Cells</p>

Abstract

Purpose: Selenium nanoparticles (SeNP) have several applications in the field of biotechnology, including their use as anti-cancer drugs. The purpose of the present study is to analyze the efficacy of green synthesis on the preparation of SeNP and its effect on their anti-cancer properties. Methods: A bacterial strain isolated from a freshwater source was shown to efficiently synthesize SeNP with potential therapeutic properties. The quality and stability of the NP were studied by scanning electron microscopy, X-ray diffraction, zeta-potential and FTIR analysis. A cost-effective medium formulation from biowaste having 6% banana peel extract enriched with 0.25 mM tryptophan was used to synthesize the NP. The NP after optimization was used to analyze their anti-tumor and anti-angiogenic activity. For this purpose, first, the cytotoxicity of the NP against cancer cells was analyzed by MTT assay and then chorioallantoic membrane assay was performed to assess anti-angiogenic activity. Further, cell migration assay and clonogenic inhibition assay were performed to test the anti-tumor properties of SeNP. To assess the cytotoxicity of SeNP on healthy RBC, hemolysis assay was performed. Results: The strain identified as Pseudomonas stutzeri (MH191156) produced phenazine carboxylic acid, which aids the conversion of Se oxyanions to reduced NP state, resulting in particles in the size range of 75 nm to 200 nm with improved stability and quality of SeNP, as observed by zeta (ξ) potential of the particles which was found to be −46.2 mV. Cytotoxicity of the SeNP was observed even at low concentrations such as 5 µg/mL against cervical cancer cell line, ie, HeLa cells. Further, neovascularization was inhibited by upto 30 % in CAMs of eggs coinoculated with SeNp when compared with untreated controls, indicating significant anti-angiogenic activity of SeNP. The NP also inhibited the invasiveness of HeLa cells as observed by decreased cell migration and clonogenic proliferation. These observations indicate significant anti-tumor and anti-angiogenic activity of the SeNP in cervical cancer cells. Conclusion: P. stutzeri (MH191156) is an efficient source of Se NP production with potential anti-angiogenic and anti-tumor properties, particularly against cervical cancer cells.

Using Green Biosynthesized Lycopene-Coated Selenium Nanoparticles to Rescue Renal Damage in Glycerol-Induced Acute Kidney Injury in Rats

“…This amorphous character in agreement with the earlier studies carried out with Withania somnifera 39 and Bacillus sp . 40 Moreover, TEM is employed to analyze the shape of the LYC-SeNPs biosynthesized LYC. TEM image of LYC-SeNPs revealed spherical particles within the diameter <130 nm.…”

Activity of bacteria isolated from bats against Pseudogymnoascus destructans in China

“…P. fluorescens has been found to only synthesize PCA; however, P. chlororaphis and P. aeruginosa can also produce some other phenazine derivatives, such as phenazine-1-amide (PCN), pyocyanin (PYO), 1-hydroxyphenazine (1-OH-PhZ), 2-hydroxy-phenazine-1-carboxylic acid (2-OH-PCA), which all have demonstrated antimicrobial activity (Mavrodi et al, 2006;Mossine et al, 2018). However, some phenazine compounds also have anti-cancer, antimalarial and anti-parasitic biological activities, which has attracted increasing attention to these compounds (Haas, et al, 2000;Laursen and Nielsen, 2004;Rajkumar et al, 2020). Challenge assays results showed that the P. yamanorum GZD 14026 isolated from the skin of M. petax during hibernation showed the strongest inhibitory effect, with an inhibition rate of 71.33%.…”

Selenium Nanoparticles for Biomedical Applications: From Development and Characterization to Therapeutics

“…SW30, [ 89 ] Bacillus pumilus sp. BAB‐3706, [ 90 ] Pseudomonas putida KT2440, [ 85 ] Azospirillum brasilense , [ 91 ] Bacillus licheniformis , [ 35 ] Vibrio natriegens , [ 92 ] Rhodococcus aetherivorans , [ 80 ] Pseudomonas alcaliphila , [ 93 ] Alcaligenes faecalis , [ 94 ] Pseudomonas stutzeri [ 95 ] and, more recently, Bacillus amyloliquefaciens SRB04, which has been shown to produce SeNPs smaller than 50 nm. [ 75 ]…”