Using Green Biosynthesized Lycopene-Coated Selenium Nanoparticles to Rescue Renal Damage in Glycerol-Induced Acute Kidney Injury in Rats

Abstract

Purpose Selenium nanoparticles (SeNPs) have recently gained much attention in nanomedicine applications owing to their unique biological properties. Biosynthesis of SeNPs using nutraceuticals as lycopene (LYC) maximizes their stability and bioactivities. In this context, this study aimed to elucidate the renoprotective activity of SeNPs coated with LYC (LYC-SeNPs) in the acute kidney injury (AKI) model. Methods Rats were divided into six groups: control, AKI (glycerol-treated), AKI+sodium selenite (Na 2 SeO 3 ; 0.5 mg/kg), AKI+LYC (10 mg/kg), AKI+LYC-SeNPs (0.5 mg/kg) and treated for 14 days. Results Glycerol treatment evoked significant increases in rhabdomyolysis-related markers (creatine kinase and LDH). Furthermore, relative kidney weight, Kim-1, neutrophil gelatinase-associated lipocalin (NGAL), serum urea, and creatinine in the AKI group were elevated. Glycerol-injected rats displayed declines in reduced glutathione level, and superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase activities, paralleled with downregulations in Nfe2l2 and Hmox-1 expressions and high renal MDA and NO contents. Glycerol-induced renal inflammation was evident by rises in TNF-α, IL-1β, IL-6, and upregulated Nos2 expression. Also, apoptotic (elevated caspase-3, Bax, and cytochrome-c with lowered Bcl-2) and necroptotic (elevated Pipk3 expression) changes were reported in damaged renal tissue. Co-treatment with Na 2 SeO 3 , LYC, or LYC-SeNPs restored the biochemical, molecular, and histological alterations in AKI. In comparison with Na 2 SeO 3 or LYC treatment, LYC-SeNPs had the best nephroprotective profile. Conclusion Our findings authentically revealed that LYC-SeNPs co-administration could be a prospective candidate against AKI-mediated renal damage via antioxidant, anti-inflammatory, anti-apoptotic and anti-necroptotic activities.

Neuroprotective Efficiency of Prodigiosins Conjugated with Selenium Nanoparticles in Rats Exposed to Chronic Unpredictable Mild Stress is Mediated Through Antioxidative, Anti-Inflammatory, Anti-Apoptotic, and Neuromodulatory Activities

“…The prepared PDGs-SeNPs calculated crystalline size is < 134 nm using Scherrer’s equation. This amorphous character is in agreement with the earlier studies carried out with lycopene 48 and different bacterial sp. 49 Moreover, TEM is employed to analyze the shape of the PDGs-SeNPs biosynthesized with PDGs.…”

Myco-Synthesized Molluscicidal and Larvicidal Selenium Nanoparticles: A New Strategy to Control Biomphalaria alexandrina Snails and Larvae of Schistosoma mansoni with an In Silico Study on Induced Oxidative Stress

“…The XRD results revealed that the myco-synthesized SeNPs are rather more amorphous than crystalline. This amorphous nature is consistent with previous research with lycopene [ 61 ], Pseudomonas stutzeri [ 62 ], and Withania somnifera [ 63 ]. FTIR analysis revealed the interaction between P. chrysogenum metabolites and SeNPs.…”

Naringin Administration Mitigates Oxidative Stress, Anaemia and Hypertension in Lead Acetate-Induced Cardiorenal Dysfunction in Cockerel Chicks

“…NGAL is a recent marker that have been used to aid diagnosis and it has been shown to be more sensitive and speci c than classical markers such as creatinine and blood urea nitrogen (BUN). However, new biomarkers such as NGAL does not only serve as indicators of glomerular damage, but also tubular impairment(Naja et al 2021;Al-Brakati et al 2021). In this study, the kidneys of Pb treated birds showed high expression of NGAL when compared with birds co-treated with naringin.…”

“…Neutrophil gelatinase-associated lipocalin (NGAL) is a member of the lipocalin superfamily of proteins that has been extensively studied in acute kidney injury. NGAL is one of the most robustly expressed proteins in the kidney following ischemic or nephrotoxic injury in animals and human(Jia et al 2021;Kovacevic et al 2021;Naja et al 2021;Al- Brakati et al 2021). NGAL is a recent marker that have been used to aid diagnosis and it has been shown to be more sensitive and speci c than classical markers such as creatinine and blood urea nitrogen (BUN).…”