&lt;p&gt;Prediction of Targets of Curculigoside A in Osteoporosis and Rheumatoid Arthritis Using Network Pharmacology and Experimental Verification&lt;/p&gt;

Han, Jiawen; Wan, Minjie; Ma, Zhanchuan; Hu, Cong; Yi, Huanfa

doi:10.2147/dddt.s282112

Cited by 36 publications

(24 citation statements)

References 73 publications

(97 reference statements)

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“…Blocking the PI3K/Akt signaling pathway partially reversed the promoting effects of curculigoside on the osteogenic differentiation of ADSCs. Han et al [ 15 ] predicted targets of curculigoside A in osteoporosis and rheumatoid arthritis using network pharmacology and experimental verification. The results showed that curculigoside A mainly targeted the EGFR, MAP2K1, MMP2, FGFR1, and MCL1 genes.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, researchers have begun working to identify natural compounds that may promote bone formation [ 14 ]. Curculigoside is a natural phenolic glycoside compound produced by Curculigo orchioides Gaertn [ 15 ]. The pharmacological effects of curculigoside are quite extensive and include antioxidative, anti-inflammatory, antidepressant, and antiosteoporotic effects [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Curculigoside promotes osteogenic differentiation of ADSCs to prevent ovariectomized-induced osteoporosis

Wei-li¹,

Xu²

2021

J Orthop Surg Res

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Background Curculigoside is a natural phenolic glycoside compound produced by Curculigo orchioides Gaertn. This study aimed to explore the effects of curculigoside in promoting the osteogenic differentiation of adipose-derived stem cells (ADSCs) as well as the underlying mechanism. Methods ADSCs were treated with curculigoside at different concentrations (0 μmol/L, 1 μmol/L, 2.5 μmol/L, 5 μmol/L, 10 μmol/L, and 20 μmol/L), and cell viability was assessed by CCK-8 assay. Then, the alkaline phosphatase (ALP) activity was determined, and alizarin red S (ARS) staining was performed to measure the extracellular mineralization of curculigoside. Information about protein-chemical interactions is provided by the search tool for interactions of chemicals (STITCH) database. Then, LY294002 was administered to explore the mechanism by which curculigoside promotes the osteogenic differentiation of ADSCs. Western blot assays were performed to assess changes in the expression of osteogenic-related markers and the phosphorylation of PI3K and AKT. Finally, we established an ovariectomized (OVX)-induced osteoporosis mouse model and administered curculigoside to explore the effects of curculigoside in preventing bone loss in vivo. Results The CCK-8 assay indicated that curculigoside did not induce cytotoxicity at a concentration of 5 μmol/L after 48 h. The ALP and ARS results revealed that the induced group had higher ALP activity and calcium deposition than the control group. Moreover, the curculigoside group exhibited increased biomineralization, ALP activity, and ARS staining compared to the induced and control groups, and these effects were partially inhibited by LY294002. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that the target genes of curculigoside were mainly involved in the PI3K-Akt signaling pathway. PCR and western blot analysis showed that the expression of RUNX2, ALP, and Osterix was upregulated in curculigoside-treated ADSCs, but this effect was partially reversed by the PI3K inhibitor LY294002. Moreover, the curculigoside-treated group exhibited significantly increased phosphorylation of AKT to P-AKT compared with the osteogenic induction group. After treatment with curculigoside, the mice had a higher bone volume than the OVX mice, suggesting partial protection from cancellous bone loss. In addition, when LY294002 was added, the protective effects of curculigoside could be neutralized. Conclusions Curculigoside could induce the osteogenic differentiation of ADSCs and prevent bone loss in an OVX model through the PI3K/Akt signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Curculigoside promotes osteogenic differentiation of ADSCs to prevent ovariectomized-induced osteoporosis

Wei-li¹,

Xu²

2021

J Orthop Surg Res

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“…The rhizome of C. orchioides and related traditional Chinese medicine formulas with strengthening of tendons and bone functions, such as “Er-Xian-Tang”, were widely used to prevent and treat osteoporosis [ 56 ]. Network pharmacology analysis of active components in C. orchioides targeting osteoporosis predicted that curculigoside ( 32 ) and other compounds had therapy potentials for osteoporosis [ 57 , 58 , 59 ]. Shen et al (2013) reported that 100 μM curculigoside significantly enhanced the proliferation of bone marrow stromal cells (BMSCs), increased the osteogenic gene expression, and profoundly up-regulated the secretion of osteoprotegerin (OPG) [ 60 ].…”

Section: Pharmacological Propertiesmentioning

confidence: 99%

“…In another arthritis rat model induced by type II collagen, compound 32 inhibited paw swelling and arthritis scores, decreased serum pro-inflammatory factor levels of TNF-α, IL-1β, IL-6, IL-10, IL-12 and IL-17A, down-regulated the expression of JAK1, JAK3 and STAT3, and up-regulated NF-κB p65 and IκB [ 26 ]. Moreover, the anti-arthritis activity of curculigoside A ( 32 ) was further confirmed by network pharmacological analysis [ 57 ].…”

Section: Pharmacological Propertiesmentioning

confidence: 99%

Phytochemistry and Pharmacological Activity of Plants of Genus Curculigo: An Updated Review Since 2013

Wang

2021

Molecules

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The genus Curculigo, as a folk herbal medicine, has been used for many years in China, treating impotence, limb limpness, and arthritis of the lumbar and knee joints. The last systematic review of the genus Curculigo was written in 2013, scientifically categorizing the phytochemistry and biological activities. Hitherto, the original compounds and their pharmacological activities were presented as the development of this genus, but there is not an updated review. To conclude the progression of the genus Curculigo, we collected the new literature published from 2013 to 2021 in PubMed, Web of Science, Google Scholar databases, and the Chinese National Knowledge Infrastructure. The novel chlorophenolic glucosides, curculigine, phenolic glycosides, orcinosides and polysaccharides were isolated from Curculigo. The new analyzing methods were established to control the quality of Curculigo as a herbal medicine. In addition, the pharmacological effects of Curculigo focused on anti-diabetes, antibacterial, anti-inflammatory, osteoporosis, antioxidation, etc. The antitumor and neuroprotective activities were newly explored in recent years. The application of herbal medicine was gradually developed in scientific methods. The medicinal value of the genus Curculigo needs to further investigate its pharmacological mechanisms. This new review offers more insights into the exploitation of the pharmacological value of the genus Curculigo.

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“…As we know, TCM has been used in clinic for thousands of years in China and has played an important role in people's health. At present, TCM is still an efficient and reliable resource for drug discovery (Cheung, 2011;Han et al, 2020). Many studies have shown that TCM has broad prospects in the prevention and treatment of major depression disorder (Sun et al, 2018;Ye et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Integrated Network Pharmacology Analysis and Experimental Validation to Investigate the Mechanism of Zhi-Zi-Hou-Po Decoction in Depression

Bai

Zhang

et al. 2021

Front. Pharmacol.

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Zhi-Zi-Hou-Po Decoction (ZZHPD) is a well-known traditional Chinese medicine (TCM) that has been widely used in depression. However, the antidepressant mechanism of ZZHPD has not yet been fully elucidated. The purpose of this study was to explore the pharmacological mechanisms of ZZHPD acting on depression by combining ultra flow liquid chromatography with quadrupole time-of-flight mass spectrometry (UFLC-Q-TOF/MS) and network pharmacology strategy. The chemical components of ZZHPD were identified using UFLC-Q-TOF/MS, while the potential drug targets and depression-related targets were collected from databases on the basis of the identified compounds of ZZHPD. Protein-protein interaction (PPI) network, gene ontology (GO), and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analyses were used to unravel potential antidepressant mechanisms. The predicted antidepressant targets from the pharmacology-based analysis were further verified in vivo. As a result, a total of 31 chemical compounds were identified by UFLC-Q-TOF/MS; 514 promising drug targets were mined by using the Swiss Target Prediction; and 527 depression-related target genes were pinpointed by the GeneCards and OMIM databases. STRING database and Cytoscape’s topological analysis revealed 80 potential targets related to the antidepressant mechanism of ZZHPD. The KEGG pathway analysis revealed that the antidepressant targets of ZZHPD were mainly involved in dopaminergic synapse, serotonin synapse, cAMP, and mTOR signaling pathways. Furthermore, based on the animal model of depression induced by chronic corticosterone, the regulatory effects of ZZHPD on the expression of MAOA, MAOB, DRD2, CREBBP, AKT1, MAPK1, HTR1A, and GRIN2B mRNA levels as well as the cAMP signaling pathway and monoaminergic metabolism were experimentally verified in rats. Our study revealed that ZZHPD is expounded to target various genes and pathways to perform its antidepressant effect.

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<p>Prediction of Targets of Curculigoside A in Osteoporosis and Rheumatoid Arthritis Using Network Pharmacology and Experimental Verification</p>

Cited by 36 publications

References 73 publications

Curculigoside promotes osteogenic differentiation of ADSCs to prevent ovariectomized-induced osteoporosis

Curculigoside promotes osteogenic differentiation of ADSCs to prevent ovariectomized-induced osteoporosis

Phytochemistry and Pharmacological Activity of Plants of Genus Curculigo: An Updated Review Since 2013

Integrated Network Pharmacology Analysis and Experimental Validation to Investigate the Mechanism of Zhi-Zi-Hou-Po Decoction in Depression

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