&lt;p&gt;Phillygenin, a MELK Inhibitor, Inhibits Cell Survival and Epithelial–Mesenchymal Transition in Pancreatic Cancer Cells&lt;/p&gt;

Li, Hongchun; Chen, Miao; Yang, Zhuying; Wang, Qinxian; Wang, Jiesheng; Dong, Jin; Yang, Xiuyun; Chen, Fuxing; Zhou, Xiumin; Luo, Kexue

doi:10.2147/ott.s238958

Cited by 18 publications

(10 citation statements)

References 35 publications

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“…EMT indicates a biological process in which the epithelial cells gain the properties of mesenchymal cells when cells undergo embryonic development or pathological processes [ 14 , 29 ]. During the EMT process, lots of alterations happen in cellular phenotypes and gene expressions.…”

Section: Discussionmentioning

confidence: 99%

Harmine suppresses the malignant phenotypes and PI3K activity in breast cancer

Yao

et al. 2022

Anti-Cancer Drugs

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Breast cancer remains a serious threaten to the women’s health, discovery of potent treatment would help to improve the outcomes of breast cancer patients. Harmine extracted from Peganum harmala L, has been reported to exert tumor suppressive activity in several malignancies. Our objective was to demonstrate the effects of harmine on the malignant phenotypes of breast cancer cells. Breast cancer cell lines (MDA-MB-231, SKBR3, and MCF-7) and human normal breast cell line MCF-10A were employed in the present study. The MTT and colony formation assays were applied to the detection of cell viability and proliferation. Wound healing and transwell assays were performed to evaluate the alterations of cell migration and invasion after harmine treatment. Flow cytometry was applied to assess the effect of harmine in inducing cell apoptosis. Furthermore, western blotting assay was used to detect the biomarkers of epithelial-mesenchymal transition and phosphatidylinositol 3 kinase (PI3K) signaling pathway. The tumorigenesis ability was detected by subcutaneous implantation. Harmine dose-dependently suppressed the viability and proliferative capacity of breast cancer cells. Flow cytometry showed that harmine induced apoptosis in MCF-7 and MDA-MB-231 cells. In addition, harmine effectively inhibited the migration and invasion abilities of breast cancer cells. Western blotting indicated harmine significantly promoted E-cadherin and PTEN expression, while suppressed N-cadherin, vimentin, PI3K, p-mTOR, and AKT levels. Interfering the PTEN expression by siRNA partly rescued the activity of PI3K signaling pathway. Moreover, harmine injection also suppressed the tumorigenesis of breast cancer cells. Our results suggested that Hermine could suppress multiple malignant phenotypes and inhibit PI3K signaling, which supports that harmine might be a potential tumor-suppressive natural compound against breast cancer.

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Section: Discussionmentioning

confidence: 99%

Harmine suppresses the malignant phenotypes and PI3K activity in breast cancer

Yao

et al. 2022

Anti-Cancer Drugs

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“…It is reported that phillygenin is an important active ingredient of lignans with a fingerprint component in Forsythiae Fructus [ 4 ]. Furthermore, it is reported as a wide-used protector with various functions, such as antiviral, antioxidant, anti-inflammatory, anticancer, and antifibrosis effects [ 4 – 8 ]. MicroRNAs (miRs) are mainly negative regulators of their target genes through binding to 3'untranslated regions (3'UTRs) [ 9 – 12 ].…”

Section: Introductionmentioning

confidence: 99%

Phillygenin Protects the Intestinal Barrier from Dysfunction via let-7b Signaling Pathway and Regulation of Intestinal Microbiota

Feng

Wang

et al. 2022

Journal of Healthcare Engineering

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The study investigates the positive effects of phillygenin on intestinal tight junction via the let-7b signaling pathway and the regulation of intestinal microbiota. The expression levels of tight junction proteins are determined through PCR and Western blot. DSS-induced mice colitis is used to verify the protective effects of phillygenin on intestinal barrier and tight junction. Fecal microbiota transplantation is used to verify the role intestinal microbiota. let-7b is detected in the colon tissues of patients with acute stercoral obstruction. Phillygenin could promote the expression of occludin, which might be inhibited by let-7b inhibitor. DSS-induced mice colitis showed that phillygenin could lower the colonic permeability and maintain the tight junction-associated proteins. The effects of phillygenin could be deprived by anti-let-7b and rescued by FMT of normal intestinal microbiota. Clinical samples verified a lower level of let-7b in stercoral obstruction patients. Phillygenin could protect the intestinal barrier from dysfunction via the signaling pathway of let-7b by regulating intestinal microbiota.

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“…The Toll-like receptor (TLR)4/MyD88/NF-κB signaling pathway, which can be activated by PHI, can inhibit lipopolysaccharide (LPS)-induced inflammation and further alleviate liver fibrosis (8). Furthermore, PHI has been reported to have significant antitumor effects in human esophageal and pancreatic cancer types (10,11).…”

Section: Introductionmentioning

confidence: 99%