Phillygenin Protects the Intestinal Barrier from Dysfunction via let-7b Signaling Pathway and Regulation of Intestinal Microbiota

Abstract

The study investigates the positive effects of phillygenin on intestinal tight junction via the let-7b signaling pathway and the regulation of intestinal microbiota. The expression levels of tight junction proteins are determined through PCR and Western blot. DSS-induced mice colitis is used to verify the protective effects of phillygenin on intestinal barrier and tight junction. Fecal microbiota transplantation is used to verify the role intestinal microbiota. let-7b is detected in the colon tissues of patients with acute stercoral obstruction. Phillygenin could promote the expression of occludin, which might be inhibited by let-7b inhibitor. DSS-induced mice colitis showed that phillygenin could lower the colonic permeability and maintain the tight junction-associated proteins. The effects of phillygenin could be deprived by anti-let-7b and rescued by FMT of normal intestinal microbiota. Clinical samples verified a lower level of let-7b in stercoral obstruction patients. Phillygenin could protect the intestinal barrier from dysfunction via the signaling pathway of let-7b by regulating intestinal microbiota.

Forsythia suspensa (Thunb.) Vahl extract ameliorates ulcerative colitis via inhibiting NLRP3 inflammasome activation through the TLR4/MyD88/NF‐κB pathway

“…Previous studies have found that Forsythia suspensa Fructus has the potential for treatment of intestinal inflammation 16 . Recently, Feng et al have reported that PHI alleviated dextran sulfate sodium (DSS)‐induced UC through the Nrf2‐NLRP3 pathway and improved metabolic dysfunction 17–19 . In spite of this, the mechanism of PHI in the treatment of UC has not been thoroughly studied.…”

“… 16 Recently, Feng et al have reported that PHI alleviated dextran sulfate sodium (DSS)‐induced UC through the Nrf2‐NLRP3 pathway and improved metabolic dysfunction. 17 , 18 , 19 In spite of this, the mechanism of PHI in the treatment of UC has not been thoroughly studied. In the current study, we performed a more detailed study on the pharmacological impact and mechanism of PHI on colitis.…”

Retracted: Phillygenin Protects the Intestinal Barrier from Dysfunction via let‐7b Signaling Pathway and Regulation of Intestinal Microbiota

“…This article has been retracted by Hindawi following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process: Discrepancies in scope Discrepancies in the description of the research reported Discrepancies between the availability of data and the research described Inappropriate citations Incoherent, meaningless and/or irrelevant content included in the article Peer-review manipulation …”

“…Tis article has been retracted by Hindawi following an investigation undertaken by the publisher [1]. Tis investigation has uncovered evidence of one or more of the following indicators of systematic manipulation of the publication process:…”