<p>Multilayered Titanium Carbide MXene Film for Guided Bone Regeneration</p>

Abstract

Purpose: MXenes are two-dimensional (2D) materials that are increasingly being applied in biomedical fields. This is ascribed to their good physiochemical properties, unique structure and high biological compatibility. However, the osteogenic activity and suitability of these materials for bone tissue engineering are not clearly understood. Thus, the aim of this study is to evaluate the biocompatibility, osteoinductivity and guided bone regeneration ability of Ti 3 C 2 T x MXene in vitro and in vivo. Methods: Multilayered Ti 3 C 2 T x MXene films were prepared and characterized by XRD and SEM. In vitro experiments were performed to evaluate the effect of MXene films on cell adhesion and morphology with SEM and fluorescence microscopy. The cytotoxicity of MXene films was detected with the Live/Dead double-staining tests. The EdU assay was employed to evaluate cell proliferation on MXene films and ALP activity was tested to determine the effect of the films on osteogenic differentiation in vitro. The mRNA expression of osteogenic differentiation-related markers was measured using qRT-PCR. In vivo animal studies were performed in which the MXene films were implanted subcutaneously in rats to evaluate biocompatibility and host tissue response in vivo. In addition, a rat calvarial defect model was established to examine the bone regeneration performance of the Ti 3 C 2 T x MXene films. The specimens were analyzed with micro-CT evaluation and histological tests. Results: The XRD and SEM analyses revealed that the Ti 3 C 2 T x MXene film was successfully synthesized. The cellular experiments showed that MXene films were highly cytocompatible and enhanced osteogenic differentiation in vitro. When implanted into subcutaneous sites and calvarial defect sites in rats, MXene films showed good biocompatibility, osteoinductivity and bone regeneration activity in vivo. Conclusion: In summary, this study presents new applications of MXenes in bone tissue engineering and in guided bone regeneration therapy.

Progress and biomedical applications of MXenes

“…MXenes have fewer applications in tissue engineering. The application of MXene in tissue engineering includes bone tissue engineering, [ 76,129–132 ] skin tissue engineering, [ 15 ] nerve tissue engineering, [ 133,134 ] and myocardial tissue engineering [ 135,136 ] mainly.…”

“…When implanted in the subcutaneous part and skull defect of rats, the MXene membrane showed good biocompatibility, osteoinductivity and bone regeneration activity in vivo. [ 132 ]…”

MXenes and MXene-based materials for tissue engineering and regenerative medicine: recent advances

“…42 Compared with these 2D materials, MXenes and MXene-base materials with high hydrophilicity, biodegradability, chemical stability, thermal/electrical conductivity, sorption selectivity, biocompatibility, and antibacterial activity are good candidates in the field of tissue engineering and regenerative medicine. 43 Herein, significant recent advancements pertaining to the tissue engineering and regenerative medicine applications of MXenes have highlighted as well as their important challenges and future perspectives.…”

Influence of MXene Particles with a Stacked-Lamellar Structure on Osteogenic Differentiation of Human Mesenchymal Stem Cells

“…In this study, MXene particles with a stacked 2D-flake structure were prepared by intercalation, resulting in a more favorable interlocking structure for producing composites or incorporating drugs [ 19 ]. The effects of the MXenes on the bone differentiation behavior of human mesenchymal stem cells were also evaluated [ 20 , 21 ].…”