&lt;p&gt;MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma&lt;/p&gt;

Li, Jun; Jiang, Donggen; Zhang, Qian; Peng, Shubin; Liao, Guolong; Yang, Xiangwei; Tang, Jiani; Xiong, Haiyun; Pang, Jun

doi:10.2147/cmar.s253533

Cited by 18 publications

(8 citation statements)

References 31 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MicroRNAs are considered to be related to cancer pathogenesis and progression [16], most of which are involved in regulating ccRCC growth. For example, mi-croRNA-301a hampers PTEN to hasten cell proliferation of RCC [17]. MicroRNA-122 downregulates Dicer to stimulate ccRCC metastasis [18].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-204-5p Hampers the Malignant Progression of Clear Cell Renal Cell Carcinoma through GXYLT2 Downregulation

Wu¹,

Liao²

2022

Kidney Blood Press Res

View full text Add to dashboard Cite

Introduction: At present, the mortality rate of clear cell renal cell carcinoma (ccRCC) remains high. The development of biomarkers is conducive to the early diagnosis and treatment of cancer. This research aimed to explore the role of microRNA-204-5p and downstream gene in ccRCC. Methods: We used bioinformatics analysis and qRT-PCR for measurement of microRNA-204-5p, qRT-PCR and western blot for GXYLT2 mRNA and protein levels, respectively. We conducted in vitro experiments like CCK8, colony formation, transwell, wound healing, and cell cycle assays to assess the role of microRNA-204-5p and GXYLT2 in ccRCC. Besides, the target relationship of microRNA-204-5p and GXYLT2 was confirmed through dual-luciferase assay. Results: This study disclosed that microRNA-204-5p was underexpressed in ccRCC cells and tissues, which was closely associated with prognosis of patients with ccRCC. Stable forced expression of microRNA-204-5p hindered malignant phenotypes of ccRCC cells. Further detection unfolded that microRNA-204-5p bound the 3’-UTR of GXYLT2 to repress its expression. Besides, forced expression of microRNA-204-5p restored the promoting impact of overexpression of GXYLT2 on malignant progression of ccRCC cells. Conclusion: These findings revealed the vital role of microRNA-204-5p and GXYLT2 in ccRCC progression, as well as the possibility of microRNA-204-5p in improving ccRCC prognosis and treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

MicroRNA-204-5p Hampers the Malignant Progression of Clear Cell Renal Cell Carcinoma through GXYLT2 Downregulation

Wu¹,

Liao²

2022

Kidney Blood Press Res

View full text Add to dashboard Cite

show abstract

“…Cell proliferation was examined by Cell Counting Kit-8 (CCK-8) assay, colony formation assay, cell cycle analysis and 5-ethynyl-2-deoxyuridine (EdU) incorporation assay, as described previously [18]. Cell cycle analysis was conducted using FACS cytometry (Beckman Coulter, USA).…”

Section: Cell Proliferation and Cell Cycle Analysismentioning

confidence: 99%

Overexpression of PFKFB3 Promotes Cell Glycolysis and Proliferation in Renal Cell Carcinoma

Li¹,

Zhang²,

Liao³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Cancer cells prefer aerobic glycolysis to increase their biomass and sustain uncontrolled proliferation. As a key glycolytic activator, phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) has been implicated in the progression of multiple types of tumors. However, the specific function and clinical significance of PFKFB3 in renal cell carcinoma (RCC) remain unclear. In the present study, we explored the role of PFKFB3 in RCC.Methods: We analyzed the expression of PFKFB3 in clear cell renal cell carcinoma (ccRCC) tissues and its relationship with clinical characteristics of ccRCC. Real-time PCR and Western blot analysis were used to detect PFKFB3 expression levels in different RCC cell lines. Furthermore, we determined the glycolytic activity by glucose uptake, lactate secretion assay and ECAR analysis. CCK-8 assay, clone formation assay, flow cytometry and EdU assay were performed to monitor cancer cell proliferation and cell cycle distribution. In addition, nude mice xenograft model was used to investigate the role of PFKFB3 in tumor growth in vivo.Results: In this study, we found that PFKFB3 was significantly up-regulated in RCC tissues and cell lines compared with normal control. Overexpression of PFKFB3 was positively associated with advanced TNM stage and could predict poor prognosis of ccRCC patients. Furthermore, knockdown of PFKFB3 suppresses cell glycolysis, proliferation and cell cycle G1/S transition in RCC cells. Importantly, in vivo experiments confirmed that PFKFB3 knockdown delayed tumor growth derived from the ACHN cell line.Conclusion: Our results suggest that PFKFB3 plays an important role in the progression of RCC via mediating glycolysis and proliferation, and provides a potential therapeutic target for RCC.

show abstract

“…Over-expression of this miRNA has been associated with advanced stage and poor survival of RCC patients. Mechanistically, miR-301a has been displayed to target PTEN tumor suppressor (22). Two other oncomiRs, namely, miR-22 and miR-193a-3p also suppress expression of PTEN in RCC cells (23,24).…”

Section: Up-regulated Mirnas In Rccmentioning

confidence: 99%

MicroRNA Signature in Renal Cell Carcinoma

et al. 2020

View full text Add to dashboard Cite

Renal cell carcinoma (RCC) includes 2.2% of all diagnosed cancers and 1.8% of cancer-related mortalities. The available biomarkers or screening methods for RCC suffer from lack of sensitivity or high cost, necessitating identification of novel biomarkers that facilitate early diagnosis of this cancer especially in the susceptible individuals. MicroRNAs (miRNAs) have several advantageous properties that potentiate them as biomarkers for cancer detection. Expression profile of miRNAs has been assessed in biological samples from RCC patients. Circulatory or urinary levels of certain miRNAs have been proposed as markers for RCC diagnosis or follow-up. Moreover, expression profile of some miRNAs has been correlated with response to chemotherapy, immunotherapy or targeted therapeutic options such as sunitinib. In the current study, we summarize the results of studies that assessed the application of miRNAs as biomarkers, therapeutic targets or modulators of response to treatment modalities in RCC patients.

show abstract

<p>MiR-301a Promotes Cell Proliferation by Repressing PTEN in Renal Cell Carcinoma</p>

Cited by 18 publications

References 31 publications

MicroRNA-204-5p Hampers the Malignant Progression of Clear Cell Renal Cell Carcinoma through GXYLT2 Downregulation

MicroRNA-204-5p Hampers the Malignant Progression of Clear Cell Renal Cell Carcinoma through GXYLT2 Downregulation

Overexpression of PFKFB3 Promotes Cell Glycolysis and Proliferation in Renal Cell Carcinoma

MicroRNA Signature in Renal Cell Carcinoma

Contact Info

Product

Resources

About