<p>MiR-223-3p targeting epithelial cell transforming sequence 2 oncogene inhibits the activity, apoptosis, invasion and migration of MDA-MB-468 breast cancer cells</p>

Abstract

PurposeThis research was to investigate the role of miR-223-3p targeting epithelial cell transforming sequence 2 oncogene (ECT2) in activity, apoptosis, invasion and migration of MDA-MB-468 breast cancer (BC) cells.MethodsThe human BC cell lines MDA-MB-468 were used for the experiment. They were divided into six groups: blank group (no plasmid transfection), NC group (negative control, transfected empty plasmid), miR-223-3p mimic group (transfected miR-223-3p mimic plasmid), miR-223-3p inhibitor group (transfected miR-223-3p inhibitor plasmid), si-ECT2 group (transfected si ECT2 plasmid) and miR-223-3p mimic+oe-ECT2 group (transfected with miR-223-3p mimic plasmid and ECT2 plasmid).ResultsCompared with the NC group, the mRNA and protein expression of Bax in miR-223-3p mimic and si-ECT2 groups were significantly increased, while the mRNA and protein expression of ECT2, Bcl-2, vascular endothelial growth factor (VEGF), and TGF-β1 were significantly decreased (all P<0.05). Compared with the NC group, the expression of miR-223-3p and the mRNA and protein expression of Bax were significantly decreased in the miR-223-3p inhibitor group, while the mRNA and protein expression of ECT2, Bcl-2, VEGF and TGF-β1 were significantly increased (both P<0.05). Compared with the single processing group, the mRNA and protein expression of Bax in the miR-223-3p mimic+si-ECT2 group were significantly increased, while the mRNA and protein expression of ECT2, Bcl-2, VEGF, and TGF-β1 were significantly decreased (all P<0.05).ConclusionMiR-223-3p targets and inhibits the expression of ECT2, thus inhibiting the invasion and migration of BC cells, and promoting cell apoptosis. miR-223-3p plays a protective role in BC.

MiR‐223‐3p inhibits rTp17‐induced inflammasome activation and pyroptosis by targeting NLRP3

“…**P < .01 and ***P < .001 vs healthy controls, Student's t test several tumour types and is known to be involved in regulating cell growth and apoptosis. 25,26 Furthermore, previous studies have shown that miR-223-3p has the ability to dampen the inflammatory response. 27,28 However, its role in syphilis is poorly understood.…”

“…Currently, miRNAs have emerged as an important therapeutic target for many diseases, including metabolic diseases, inflammation and cancer. MiR‐223‐3p was found to be down‐regulated in several tumour types and is known to be involved in regulating cell growth and apoptosis 25,26 . Furthermore, previous studies have shown that miR‐223‐3p has the ability to dampen the inflammatory response 27,28 .…”

Upregulation of CCT-3 Induces Breast Cancer Cell Proliferation Through miR-223 Competition and Wnt/β-Catenin Signaling Pathway Activation

“…Evidences show that miR-223 is a tumor suppressor gene in breast cancer. One of the studies reported that miR-223 regulates cancer cell invasion and migration through targeting ECT2 ( 34 ). Fabris et al showed that miR-223 could inhibit the recurrence of breast cancer by mediating the EGF signaling pathway in their study ( 35 ).…”

MicroRNA related prognosis biomarkers from high throughput sequencing data of kidney renal clear cell carcinoma

“…Previous studies indicated that hsa-miR-223-3p and hsa-miR-365a-3p could promote proliferation, migration and invasion of cancer cells [ 28 – 31 ]. But what very interesting is that previous studies also indicated that hsa-miR-223-3p and hsa-miR-365a-3p could inhibit the invasion and migration of cancer cells [ 32 – 35 ]. All of those results suggested that the same gene or miRNA may play different roles in different cancers.…”