&lt;p&gt;miR-1271 inhibits growth, invasion and epithelial–mesenchymal transition by targeting ZEB1 in ovarian cancer cells&lt;/p&gt;

Yang, Jiao; Zhu, Guiping; Jiang, Yu; Liu, Ying; Wu, Man; Zhao, Jing; Tian, Xiangwen

doi:10.2147/ott.s219018

Cited by 14 publications

(10 citation statements)

References 37 publications

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“…In the case of osteosarcoma, it was demonstrated that miR-1271-5p inhibited cell proliferation and invasion by Wnt signaling [ 53 ]. A study in cell cultures reports inhibition of miR-1271-5p expression in ovarian cancer cells compared to normal [ 54 ].…”

Section: Resultsmentioning

confidence: 99%

miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer

et al. 2020

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Background Prostate cancer is one of the most common and socially significant cancers among men. The aim of our study was to reveal changes in miRNA expression profiles associated with lymphatic dissemination in prostate cancer and to identify the most prominent miRNAs as potential prognostic markers for future studies. Methods High-throughput miRNA sequencing was performed for 44 prostate cancer specimens taken from Russian patients, with and without lymphatic dissemination (N1 – 20 samples; N0 – 24 samples). Results We found at least 18 microRNAs with differential expression between N0 and N1 sample groups: miR-182-5p, miR-183-5p, miR-96-5p, miR-25-3p, miR-93-5p, miR-7-5p, miR-615-3p, miR-10b, miR-1248 (N1-miRs; elevated expression in N1 cohort; p < 0.05); miR-1271-5p, miR-184, miR-222-3p, miR-221-5p, miR-221-3p, miR-455-3p, miR-143-5p, miR-181c-3p and miR-455-5p (N0-miRs; elevated expression in N0; p < 0.05). The expression levels of N1-miRs were highly correlated between each other (the same is applied for N0-miRs) and the expression levels of N0-miRs and N1-miRs were anti-correlated. The tumor samples can be divided into two groups depending on the expression ratio between N0-miRs and N1-miRs. Conclusions We found the miRNA expression signature associated with lymphatic dissemination, in particular on the Russian patient cohort. Many of these miRNAs are well-known players in either oncogenic transformation or tumor suppression. Further experimental studies with extended sampling are required to validate these results.

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Section: Resultsmentioning

confidence: 99%

miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer

et al. 2020

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“…As for EOC, our previous study uncovered that circ-ABCB10 expression is elevated in EOC cell lines compared with normal ovarian epithelial cells, and it increases cell proliferation, but decreases apoptosis and the expression of miR-1271, miR-1252 and miR-203 (9). miR-1271 serves as a tumor suppressive miRNA, which inhibits cell proliferation and invasion via modulating downstream target genes in various types of cancer (10,16,17). In EOC, miR-1271 inhibits cell viability, invasion and epithelial mesenchymal transition by directly binding to the 3'-untranslated region of zinc finger E-box binding homeobox 1 mRNA in EOC (17).…”

Section: Discussionmentioning

confidence: 99%

“…miR-1271 serves as a tumor suppressive miRNA, which inhibits cell proliferation and invasion via modulating downstream target genes in various types of cancer (10,16,17). In EOC, miR-1271 inhibits cell viability, invasion and epithelial mesenchymal transition by directly binding to the 3'-untranslated region of zinc finger E-box binding homeobox 1 mRNA in EOC (17). In colorectal cancer, miR-1271 promotes cell proliferation and invasion by suppressing Capn4 (10).…”

Section: Discussionmentioning

confidence: 99%

Circular RNA ABCB10 promotes cell proliferation and invasion, but inhibits apoptosis via regulating the microRNA‑1271‑mediated Capn4/Wnt/β‑catenin signaling pathway in epithelial ovarian cancer

Lin

Chen

et al. 2021

Mol Med Rep

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Circular RNA ABCB10 (circ-ABCB10) modulates cellular functions and microRNA (miR)-1271 in epithelial ovarian cancer (EOC). The present study aimed to investigate the interaction between circ-ABCB10 and miR-1271 in regulating EOC cellular function and the calpain small subunit 1 (Capn4)/Wnt/β-catenin signaling pathway. circ-ABCB10 and miR-1271 expression levels were detected in EOC cells (OVCAR3, UWB1.289, SKOV3 and CAOV3) and normal ovarian epithelial cells (IOSE80) via reverse-transcription quantitative PCR. SKOV3 cells were transfected with control short hairpin (sh)RNA plasmids, control inhibitor, circ-ABCB10 shRNA plasmids and miR-1271 inhibitor. UWB1.289 cells were transfected with control overexpression plasmids, control mimic, circ-ABCB10 overexpression plasmids and miR-1271 mimic. Subsequently, cell proliferation, apoptosis, invasion and the Capn4/Wnt/β-catenin signaling pathway were assessed. In addition, a luciferase activity assay was performed. circ-ABCB10 expression was significantly increased in OVCAR3, SKOV3 and CAOV3 cells compared with IOSE80 cells, but was not significantly altered in UWB1.289 cells. miR-1271 expression was significantly decreased in OVCAR3, UWB1.289, SKOV3 and CAOV3 cells compared with IOSE80 cells. In both SKOV3 and UWB1.289 cells, circ-ABCB10 negatively regulated miR-1271, whereas miR-1271 did not affect circ-ABCB10. Furthermore, circ-ABCB10 enhanced cell proliferation, invasion and the Capn4/Wnt/β-catenin signaling pathway, but inhibited cell apoptosis, whereas miR-1271 suppressed cell proliferation, invasion and the Capn4/Wnt/β-catenin signaling pathway, but facilitated cell apoptosis. Moreover, miR-1271 attenuated the proproliferative, proinvasive and antiapoptotic effects of circ-ABCB10, and reversed the positive regulation of circ-ABCB10 on the Capn4/Wnt/β-catenin signaling pathway. Besides, the luciferase activity assay indicated that circ-ABCB10 directly bound to miR-1271. In conclusion, the present study indicated that circ-ABCB10 promoted cell proliferation and invasion, and suppressed apoptosis by regulating the miR-1271-mediated Capn4/Wnt/β-catenin signaling pathway in EOC.

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“…Chen et al indicated that miR-1271 blocks the development of papillary thyroid carcinoma via inhibiting IRS1 expression and inactivating the AKT signaling pathway [ 27 ]. Importantly, miR-1271 has been reported as a suppressive factor in OC cells, which suppresses growth and invasion through mediating epithelial-mesenchymal transition [ 28 ]. However, the specific molecular mechanism of miR-1271-5p in OC still needed to be detected.…”

Section: Discussionmentioning

confidence: 99%

microRNA-1271-5p/TIAM1 suppresses the progression of ovarian cancer through inactivating Notch signaling pathway

Han

Shen

et al. 2020

J Ovarian Res

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Objective Ovarian cancer (OC) has been regarded as the most malignant gynecological neoplasm and often confers grave outcomes owing to the frequent metastasis and high recurrence. A previous study has demonstrated that miR-1271-5p is implicated in OC progression, however, the possible mechanism of it remains unknown. The purpose of this investigation was to explore how miR-1271-5p regulates the progression of OC. Methods Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases were employed to analyze the differentially expressed miRNAs or genes as well as their corresponding prognostic values. miR-1271-5p expression in OC cells was examined by qRT-PCR. Cell counting kit 8 (CCK-8), colony formation, and transwell tests were conducted to evaluate the proliferation, migration and invasion potentials. Bioinformatics prediction and luciferase activity analysis were utilized to predict and verify the target gene of miR-1271-5p. Western blot assay was carried out to measure protein expression. Results miR-1271-5p was significantly decreased in OC and its down-regulation was associated with the grave outcome of OC patients. Upregulation of miR-1271-5p inhibited cell viability, but miR-1271-5p knockdown promoted the proliferation of OC cells. TIAM1 was a direct target gene of miR-1271-5p and expressed in OC tissues at higher level. High expression of TIAM1 induced the poorer prognosis of patients with OC. Further functional analyses showed that the suppressive role of miR-1271-5p on OC cell malignant behaviors was overturned by the upregulation of TIAM1. The protein levels of Cyclin D1, HES1, NOTCH and NUMB were remarkably changed due to the abnormal expression of miR-1271-5p and TIAM1. Conclusion To sum up, miR-1271-5p inhibits proliferation, invasion and migration of OC cells by directly repressing TIAM1 to inactivate the Notch signaling pathway, which provides an alternative therapeutic candidate for the advancement of OC treatment.

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<p>miR-1271 inhibits growth, invasion and epithelial–mesenchymal transition by targeting ZEB1 in ovarian cancer cells</p>

Cited by 14 publications

References 37 publications

miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer

miRNAs expression signature potentially associated with lymphatic dissemination in locally advanced prostate cancer

Circular RNA ABCB10 promotes cell proliferation and invasion, but inhibits apoptosis via regulating the microRNA‑1271‑mediated Capn4/Wnt/β‑catenin signaling pathway in epithelial ovarian cancer

microRNA-1271-5p/TIAM1 suppresses the progression of ovarian cancer through inactivating Notch signaling pathway

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