Circular RNA ABCB10 promotes cell proliferation and invasion, but inhibits apoptosis via regulating the microRNA‑1271‑mediated Capn4/Wnt/β‑catenin signaling pathway in epithelial ovarian cancer

Abstract

Circular RNA ABCB10 (circ-ABCB10) modulates cellular functions and microRNA (miR)-1271 in epithelial ovarian cancer (EOC). The present study aimed to investigate the interaction between circ-ABCB10 and miR-1271 in regulating EOC cellular function and the calpain small subunit 1 (Capn4)/Wnt/β-catenin signaling pathway. circ-ABCB10 and miR-1271 expression levels were detected in EOC cells (OVCAR3, UWB1.289, SKOV3 and CAOV3) and normal ovarian epithelial cells (IOSE80) via reverse-transcription quantitative PCR. SKOV3 cells were transfected with control short hairpin (sh)RNA plasmids, control inhibitor, circ-ABCB10 shRNA plasmids and miR-1271 inhibitor. UWB1.289 cells were transfected with control overexpression plasmids, control mimic, circ-ABCB10 overexpression plasmids and miR-1271 mimic. Subsequently, cell proliferation, apoptosis, invasion and the Capn4/Wnt/β-catenin signaling pathway were assessed. In addition, a luciferase activity assay was performed. circ-ABCB10 expression was significantly increased in OVCAR3, SKOV3 and CAOV3 cells compared with IOSE80 cells, but was not significantly altered in UWB1.289 cells. miR-1271 expression was significantly decreased in OVCAR3, UWB1.289, SKOV3 and CAOV3 cells compared with IOSE80 cells. In both SKOV3 and UWB1.289 cells, circ-ABCB10 negatively regulated miR-1271, whereas miR-1271 did not affect circ-ABCB10. Furthermore, circ-ABCB10 enhanced cell proliferation, invasion and the Capn4/Wnt/β-catenin signaling pathway, but inhibited cell apoptosis, whereas miR-1271 suppressed cell proliferation, invasion and the Capn4/Wnt/β-catenin signaling pathway, but facilitated cell apoptosis. Moreover, miR-1271 attenuated the proproliferative, proinvasive and antiapoptotic effects of circ-ABCB10, and reversed the positive regulation of circ-ABCB10 on the Capn4/Wnt/β-catenin signaling pathway. Besides, the luciferase activity assay indicated that circ-ABCB10 directly bound to miR-1271. In conclusion, the present study indicated that circ-ABCB10 promoted cell proliferation and invasion, and suppressed apoptosis by regulating the miR-1271-mediated Capn4/Wnt/β-catenin signaling pathway in EOC.

The Role of ARID1A in Tumors: Tumor Initiation or Tumor Suppression?

“…Breast cancer is a common type of cancer worldwide, which still ranks as the second most prevalent leading cause of cancer-related mortality among females ( 62 , 63 ). ARID1A plays an important pathobiological role in breast cancer, and the partial loss of its expression is associated with unfavorable outcome in patients with breast cancer ( 64 ).…”

The functional roles of the circRNA/Wnt axis in cancer

“…Ovarian cancer (OC), endometrial cancer (EC), and cervical cancer are the three most common gynecological malignancies [271][272][273][274]. The expression of circ-ABCB10 is significantly upregulated, while circPLEKHM3 expression is downregulated in OC [184,220]. Moreover, the level of circPLEKHM3 is positively associated with the overall survival rate in patients with OC [184].…”

“…Moreover, the level of circPLEKHM3 is positively associated with the overall survival rate in patients with OC [184]. Circ-ABCB10 remarkably facilitates cell proliferation and invasion and reduces cell apoptosis by miR-1271 in OC [220]. Circ-ABCB10 plays a critical role in OC progression via the regulation of Capn4/Wnt/β-catenin.…”

The Emerging Roles of Circ-ABCB10 in Cancer

“…Among the target miRNAs that can be regulated by circRNAs, it was demonstrated that miR-1252 can provide potential biomarkers and therapeutic targets in non-small cell lung cancer (Tian et al, 2019) and epithelial ovarian cancer (Chen et al, 2019). Similarly, it was also found that circ-ABCB10 can sponge miR-1271, which may have a complementary sequence, possibly regulating cell proliferation and migration of breast cancer and epithelial ovarian cancer cells (Liang et al, 2017;Lin et al, 2021;Zhao et al, 2021). Furthermore, since conserved miR-1271 target sites on circ-ABCB10 are complementary to miR-1271, these sites could be a lodging site for transport (Lin et al, 2021).…”