<p>MicroRNA-766 Promotes The Proliferation, Migration And Invasion, And Inhibits The Apoptosis Of Cutaneous Squamous Cell Carcinoma Cells By Targeting PDCD5</p>

Abstract

This study aimed to investigate the regulatory role and mechanism of microRNA-766 (miR-766) on cutaneous squamous cell carcinoma (CSCC) cells. Methods: The expression of miR-766 and programmed cell death 5 (PDCD5) was detected in CSCC tissues and CSCC cell lines (A431, SCL-1 and DJM-1 cells) by qRT-RCR. The proliferation, colony-forming ability, apoptosis, migration and invasion of A431 and SCL-1 cells was measured by MTT, colony formation, flow cytometry, wound healing and transwell assay, respectively. The interaction between miR-766 and PDCD5 was detected by dualluciferase reporter gene assay. The expression of matrix metalloproteinase 2 (MMP-2), MMP-9 and PDCD5 was measured by Western blot. In addition, A431 cells were subcutaneously injected into mice, and the tumor volume and weight were measured. Results: MiR-766 was upregulated, and PDCD5 was downregulated in CSCC tissues and cells. MiR-766 significantly promoted the proliferation, migration and invasion, and inhibited the apoptosis of A431 and SCL-1 cells. MiR-766 also significantly increased the expression of MMP-2 and MMP-9 in A431 and SCL-1 cells. PDCD5 was a target gene of miR-766. PDCD5 significantly reversed the tumor-promoting effect of miR-766 on A431 and SCL-1 cells. In addition, miR-766 inhibitor inhibited the tumor growth in mice. Conclusion: MiR-766 inhibitor inhibited the proliferation, migration and invasion, and promoted the apoptosis of CSCC cells via downregulating PDCD5.

Downregulation of lncRNA NEAT1 inhibits the proliferation of human cutaneous squamous cell carcinoma in vivo and in vitro

“…MMP-2 and MMP-9 genes play a significant part in squamous cell carcinoma metastasis (26). For example, microRNA (miR)-766 promotes the invasion and migration of squamous cell carcinoma cells A431 and SCL-1 by releasing MMP-2 and -9 (27). In CSCC cells, overexpression of LINC00520 significantly inhibits migratory and adhesive capacity by downregulating MMP-2 mRNA and MMP-9 protein levels (28).…”

The key role of microRNA-766 in the cancer development

“…MiR-766 sped up A431 and SCL-1 cells’ motility, invasion, and proliferation, while also boosting MMP-2 and MMP-9. As a result, miR-766 can increase proliferation migration and invasion, of CSCC cells by reducing the level of PDCD5 expression ( 104 ). Additionally, through increasing HOXA9, miR766-3p downregulation inhibited CSCC cell growth, metastasis, and glycolysis ( 174 ).…”

“…On the CSCC, the precise regulatory impact of miR-766 is still unknown. Recently, Liu et al (104), suggested that miR-766 might function in CSCC cells as an oncomiRNA. They discovered that in CSCC cells and tissues, miR-766 expression was elevated whereas PDCD5 expression was downregulated.…”

Non-coding RNAs in skin cancers:Biological roles and molecular mechanisms

“…MiR-142-5p targets PTEN ( Bai et al, 2018 ), miR-186 targets reticulophagy regulator 1 ( Xinde Hu et al, 2019 ), and miR-320a regulates autophagy related 2B ( Bi et al, 2021 ) to inhibit cancer cell apoptosis. Programmed cell death 5 is the dominant downstream target of miR-766, the upregulation of which inhibits the apoptosis of CSCC cells ( Liu et al, 2020 ). Increased expression of miR-22 decreases CSCC chemosensitivity by targeting the Wnt/β-catenin signaling axis, thus promoting malignant progression ( Yuan et al, 2021 ).…”

“…MiR-142-5p targets PTEN (Bai et al, 2018), miR-186 targets reticulophagy regulator 1 (Xinde , and miR-320a regulates autophagy related 2B (Bi et al, 2021) to inhibit cancer cell apoptosis. Programmed cell death 5 is the dominant downstream target of miR-766, the upregulation of which inhibits the apoptosis of CSCC cells (Liu et al, 2020).…”