<p>Low-Magnitude High-Frequency Vibration Decreases Body Weight Gain and Increases Muscle Strength by Enhancing the p38 and AMPK Pathways in db/db Mice</p>

Abstract

Objective: To evaluate the effect LMHFV on body weight gain, NAFLD and muscle strength and explore effect in mitochondrial biogenesis, AMPKα and p38 pathways. Methods: Vibration platform used in this study provides specific whole-body cyclic mechanical stimulation at low magnitude (0.3 g) and high frequency (50 Hz). Diabetic mice (8-9 mice per group) (C57BL/KsJ-m+/+Lepr db) were randomly divided into untreated group (no vibration) and two vibration groups. Lean mice (8 mice) were used as non-diabetic control for both groups. Two diabetic vibration groups received LMHFV every day for 20 min/day and 40 min/day separately. Results: After 8 weeks of treatment, results showed that body weight, liver weight, fat pad weight, glucose level and insulin level were lower in vibration group when compared with the untreated group. The ratio of fat in liver was significantly decreased after vibration treatment. Muscle strength was significantly increased after vibration. Mitochondrial biogenesis-related gene expression was increased in soleus, gastrocnemius and liver. AMPKα mRNA expression level was increased in soleus and gastrocnemius after vibration treatment. p38 and AMPKα mRNA expression level and protein expression level in liver were enhanced with vibration treatment. Moreover, phosphorylation of p38 and AMPKα was enhanced in liver. Conclusion: LMHFV applied in our study decreases body weight gain and improves muscle strength and NAFLD in diabetic mice which were partly through improving mitochondrial biogenesis by enhancing p38 and AMPKα pathway.

Protective effects of low-magnitude high-frequency vibration on high glucose-induced osteoblast dysfunction and bone loss in diabetic rats

“…By inducing diabetes in rats by STZ, the diabetic rats showed significantly elevated blood glucose levels and weight loss as the typical manifestations of type I diabetes mellitus [ 33 ]. With LMHFV treatment, significantly reduced blood glucose was observed, which was consistent with the results reported from previous research in STZ-diabetic rats and db/db mice [ 34 , 35 ]. However, previous studies suggested that LMHFV did not exert any weight gain effect on STZ-diabetic rabbits [ 36 ].…”

Exercise Training Enhances Myocardial Mitophagy and Improves Cardiac Function via Irisin/FNDC5-PINK1/Parkin Pathway in MI Mice

“…The mice underwent 8 rounds of training per day, 3 times of climbing the ladder per round, 1 min rest between each round, lasting 5 days per week for 4 weeks. Vibration exercise refers to the research of Ren et al [ 19 ]. Vibration platform provided vertical vibrations at 50 Hz with a peak-to-peak magnitude of 0.3 g, lasting 15 min per day, 5 days per week for 4 weeks.…”

Possible Mechanisms for the Effects of Sound Vibration on Human Health

“…Vibration may also enhance mitochondrial biogenesis, which normally occurs as a major adaptation of skeletal muscles in response to exercise training. One study found that vibration at 50 Hz enhanced the expression of PGC-1α in the soleus muscle, gastrocnemius muscle and liver, and was associated with increased muscle strength [ 162 ]. PGC1α plays an important role in mitochondrial biogenesis and is regulated by mitogen-activated protein kinase p38 (p38 MAPK) and is activated during exercise.…”

“…Gloeckl et al [ 161 ] and Lage et al [ 162 ] were able to safely and successfully use vibration therapy as a pulmonary rehabilitation tool for patients with chronic obstructive pulmonary disease (COPD) who require physical exercise but cannot perform due to breathing limitations and general malaise. Although physical exercise is the basis of pulmonary rehabilitation for people with COPD, intense exercise may also stimulates pro-inflammatory cytokines and can be harmful in that context [ 165 , 166 ].…”