&lt;p&gt;Long Non-Coding RNA SNHG14 Impedes Viability, Migration and Invasion of Endometrial Carcinoma Cells Through Modulating miR-93-5p/&lt;em&gt;ZBTB7A&lt;/em&gt; Axis&lt;/p&gt;

Zhang, Kai; Cai, Yongqin; Zhou, Qi; Sun, Hong; Wei, Jinying

doi:10.2147/cmar.s257419

Cited by 16 publications

(15 citation statements)

References 34 publications

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“…Furthermore, cell migration and invasion are key processes that are associated with metastasis of EC. 28,29 Our study confirmed the possible antimetastatic function of circ_0001610 silencing in EC by decreasing the migratory and invasive abilities. Additionally, glycolysis is an important metabolic pathway that is involved in various cellular processes in EC progression.…”

Section: Discussionsupporting

confidence: 73%

hsa_circ_0001610 knockdown modulates miR‐646‐STAT3 axis to suppress endometrial carcinoma progression

Wang¹,

Feng

2021

The Journal of Gene Medicine

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Background: Endometrial carcinoma (EC) development is associated with dysregulated circular RNA profiles. The purpose of the current research is to study the role and mechanism of hsa_circ_0001610 (circ_0001610) in EC progression. Methods: circ_0001610, microRNA (miR)-646, and signal transducer and activator of transcription 3 (STAT3) expression levels were measured in EC. Functional analyses were performed using Cell Counting Kit-8, colony formation, transwell, wound healing, flow cytometry, glycolysis, and xenograft analyses. Binding association was evaluated with dual-luciferase reporter assay. Results: circ_0001610 levels were upregulated in EC samples (n = 30) and cells. circ_0001610 interference restrained cell proliferation, migration, and invasion, and promoted apoptosis. circ_0001610 downregulation constrained glycolysis through reducing glucose consumption, lactate production, and levels of adenosine triphosphate, extracellular acidification, hexokinase 2, and lactate dehydrogenase A, and increasing oxygen consumption rate. miR-646 is targeted by circ_0001610, and miR-646 inhibition attenuated interference of circ_0001610-mediated suppression of EC development. STAT3 was modulated by miR-646, and miR-646 upregulation restrained EC progression by decreasing STAT3. circ_0001610 silencing reduced STAT3 levels by sponging miR-646 and reduced the growth of xenograft tumor established by EC cells. Conclusion: circ_0001610 knockdown represses EC progression through modulating the miR-646-STAT3 axis.

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Section: Discussionsupporting

confidence: 73%

hsa_circ_0001610 knockdown modulates miR‐646‐STAT3 axis to suppress endometrial carcinoma progression

Wang¹,

Feng

2021

The Journal of Gene Medicine

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“…When comparing the remaining potential miRNAs, according to the present bioinformatics analysis (miR-93-5p, miR-23c, miR-210-3p, miR-221-3p, and miR-592), all were suggested with important roles in PC screening [61], aggressiveness [8,62,63], and recurrence [7,18,64], or even new etiological values [65] were suggested. These miRNAs have also been highlighted with carcinogenic effects in other tumors, such as endometrial [66,67], osteosarcoma [68], hepatocellular [69], renal [17,70], or colorectal [71], among others [72][73][74]. According to our experimental analysis, miR-210-3p, miR-23c, and miR-592 were suggested as aggressiveness biomarkers in PC and miR-93-5p, miR-130b, and miR-141 were suggested as diagnostic biomarkers for this tumor.…”

Section: Discussionmentioning

confidence: 62%

Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer

et al. 2021

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MiRNAs play a relevant role in PC (prostate cancer) by the regulation in the expression of several pathways’ AR (androgen receptor), cellular cycle, apoptosis, MET (mesenchymal epithelium transition), or metastasis. Here, we report the role of several miRNAs’ expression patterns, such as miR-miR-93-5p, miR-23c, miR-210-3p, miR-221-3p, miR-592, miR-141, miR-375, and miR-130b, with relevance in processes like cell proliferation and MET. Using Trizol® extraction protocol and TaqMan™ specific probes for amplification, we performed miRNAs’ analysis of 159 PC fresh tissues and 60 plasmas from peripheral blood samples. We had clinical data from all samples including PSA, Gleason, TNM, and D’Amico risk. Moreover, a bioinformatic analysis in TCGA (The Cancer Genome Atlas) was included to analyze the effect of the most relevant miRNAs according to aggressiveness in an extensive cohort (n = 531). We found that miR-210-3p, miR-23c, miR-592, and miR-93-5p are the most suitable biomarkers for PC aggressiveness and diagnosis, respectively. In fact, according with our results, miR93-5p seems the most promising non-invasive biomarker for PC. To sum up, miR-210-3p, miR-23c, miR-592, and miR93-5p miRNAs are suggested to be potential biomarkers for PC risk stratification that could be included in non-invasive strategies such as liquid biopsy in precision medicine for PC management.

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“…It is known microRNA-17-5p plays a suppressive roles in controlling apoptosis in endometrial cancer, 33 while miR-93-5p impedes the viability, migration and invasion of endometrial carcinoma cells. 34 …”

Section: Discussionmentioning

confidence: 99%

The Circadian Gene NPAS2 Act as a Putative Tumor Stimulative Factor for Uterine Corpus Endometrial Carcinoma

Zheng¹,

Lv²,

Zhu³

et al. 2021

CMAR

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Background: Mounting evidence indicates altered circadian rhythm represents a critical factor affecting carcinogenesis and tumor progression. The circadian gene neuronal PAS domain protein 2 (NPAS2) constitutes a newly discovered prognostic biomarker. NPAS2 dysregulation is found in multiple malignancies, although its functions in uterine corpus endometrial carcinoma (UCEC) remain largely unknown. Objective: To evaluate NPAS2's roles in UCEC and to explore the underlying mechanisms. Methods: NPAS2 transcription levels, patient prognosis, different clinical stages and target microRNAs in UCEC cases were comparatively assessed based on public databases, including UALCAN, GEPIA, TIMER, Kaplan-Meier plotter, starBase database, LinkedOmics and String. Then, qRT-PCR and immunohistochemical analysis were applied to analyze the expression of NPAS2 in UCEC tissue samples. CCK-8, clonogenic assay and flow cytometry were carried out for detecting cell viability, colony formation ability and cell apoptosis, respectively. Results: NPAS2 was upregulated in tissue samples from UCEC cases compared with the corresponding control specimens. NPAS2 overexpression was associated with decreased overall (OS), disease free (DFS) and relapse free (RFS) survival in UCEC. In addition, NPAS2 overexpression was associated with clinical stage, tumor grade, estrogen receptor status, myometrial invasion in UCEC. Furthermore, NPAS2 knockdown or overexpression altered cell proliferation and apoptosis in UCEC. Moreover, NPAS2 showed significant negative correlations with miR-17-5p and miR-93-5p, and positive correlations with miR-106a-5p and miR-381-3p in UCEC. Conclusion: NPAS2 overexpression is associated with poor prognosis and clinicopathological characteristics in UCEC and promotes proliferation and colony formation while inhibiting apoptosis. Finally, NPAS2 is associated with several miRNAs in UCEC.

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<p>Long Non-Coding RNA SNHG14 Impedes Viability, Migration and Invasion of Endometrial Carcinoma Cells Through Modulating miR-93-5p/<em>ZBTB7A</em> Axis</p>

Cited by 16 publications

References 34 publications

hsa_circ_0001610 knockdown modulates miR‐646‐STAT3 axis to suppress endometrial carcinoma progression

hsa_circ_0001610 knockdown modulates miR‐646‐STAT3 axis to suppress endometrial carcinoma progression

Identification of MicroRNAs as Viable Aggressiveness Biomarkers for Prostate Cancer

The Circadian Gene NPAS2 Act as a Putative Tumor Stimulative Factor for Uterine Corpus Endometrial Carcinoma

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