<p>Long Non-Coding RNA NEAT1 Promotes the Proliferation, Migration, and Metastasis of Human Breast-Cancer Cells by Inhibiting miR-146b-5p Expression</p>

Abstract

Background: Breast cancer (BC) is the most commonly diagnosed cancer in women. Tumor recurrence and metastasis are the key causes of death in BC patients. Long noncoding RNA (lncRNA) is closely associated with BC progression. lncRNA nuclear-enriched abundant transcript (NEAT)1 has been reported to regulate the proliferation and mobility of several types of cancer cells. However, how lncRNA NEAT1 affects the proliferation and invasion of BC cells is not known. Methods: Quantitative real time-polymerase chain reaction (qRT-PCR) was used to measure expression of lncRNA NEAT1 and microRNA (miR)-146b-5p in BC tissues and cell lines. Cell Counting Kit (CCK)-8, cell colony-formation, wound-healing, and Transwell™ assays were undertaken to determine the effects of lncRNA NEAT1 and miR-146b-5p on progression of BC cells. The interaction between lncRNA NEAT1 and miR-146b-5p was examined by luciferase reporter, RNA-binding protein immunoprecipitation (RIP), and RNA-pulldown assays. Results: Expression of lncRNA NEAT1 was upregulated in BC tissues and cell lines. High expression of lncRNA NEAT1 predicted poor overall survival in BC patients. Silencing of expression of lncRNA NEAT1 inhibited epithelial-mesenchymal transition (EMT) and suppressed the proliferation, migration and invasion of BC cells. Ectopic expression of lncRNA NEAT1 induced EMT and promoted BC progression. Mechanistic investigations revealed that miR-146b-5p was a direct target of lncRNA NEAT1, and its expression was correlated negatively with expression of lncRNA NEAT1 in BC tissues. Conclusion: lncRNA NEAT1 could (i) serve as a novel prognostic marker for BC and (ii) be a potential therapeutic target for BC.

LncRNA NEAT1 Promotes High Glucose-Induced Mesangial Cell Hypertrophy by Targeting miR-222-3p/CDKN1B Axis

“…It would also be interesting to test if STAT3 regulates the expression of NEAT1 in the context of immune regulation, and if miR-222-3p mediated regulation would be physiologically important in this context as well. A recent study has shown that NEAT1 promotes cell proliferation, migration and metastasis in breast cancer by inhibiting miR-146b-5p ( Li et al, 2020 ). Our study has identified miR-222-3p as a novel target of NEAT that has important functional role in regulating hypertrophy.…”

Non-coding RNAs in necroptosis, pyroptosis and ferroptosis in cancer metastasis

“… LncRNA Neat1 miR-34c miR-146b-5p miR-224-5p miR-382-3p The lncRNA Neat1 stabilized the mature caspase-1 to promote pyroptosis [ 78 ]. Neat1 promoted metastasis in human various cancers, by inhibiting miR-146b-5p [ 161 ] in breast cancers, targeting miR-224-5p in malignant melanoma [ 162 ], by mediating miR-382-3p in ovarian cancer [ 163 ]. lncRNA MALAT1 miR-22 miR-23c lncRNA MALAT1 promoted pyroptosis as the ceRNA to competitively bind miR-22, which led to the levels of NLRP3 was affected.…”

Long non‑coding RNA ATB is associated with metastases and promotes cell invasion in colorectal cancer via sponging miR‑141‑3p

“…mRNA and miRNA expression levels were normalized to the internal reference genes GAPDH and U6, respectively. Quantification of mRNA and miRNA expression was performed by using the 2 - ΔΔ Cq method ( 28 ).…”