<p>Long Non-Coding RNA HAND2-AS1 Inhibits Growth and Migration of Gastric Cancer Cells Through Regulating the miR-590-3p/KCNT2 Axis</p>

Abstract

Introduction: Long non-coding RNAs (lncRNAs) are regarded as crucial regulators for cancer initiation and progression. Heart and Neural Crest Derivatives Expressed 2 antisense RNA 1 (HAND2-AS1) was recently proposed to function as tumor suppressor in several human cancers. However, its role in gastric cancer (GC) remains unclear. Methods: HAND2-AS1 expression in GC tissues and normal tissues was analyzed at GEPIA (a web server for gene expression profiling analysis). Moreover, RT-qPCR method was utilized to explore HAND2-AS1 expression in GC cells and normal cell. In vitro experiments were carried out using cell counting kit-8 assay, colony formation assay, and flow cytometry assay, respectively. Bioinformatic analysis and luciferase activity reporter assay were performed to identify the downstream targets of HAND2-AS1. Results: We found HAND2-AS1 has decreased expression in both GC tissues and cells. Overexpression of HAND2-AS1 was able to inhibit GC cell proliferation, colony formation, but promote apoptosis. On the contrary, knockdown of HAND2-AS1 could cause the opposite effects on GC cells. Furthermore, HAND2-AS1 was shown to function as a competitive RNA that binds with microRNA-590-3p (miR-590-3p) to affect the expression of potassium sodium-activated channel subfamily T member 2 (KCNT2). Discussion: Our results indicated the tumor suppressive role of HAND2-AS1 in GC. Also, the newly identified HAND2-AS1/miR-590-3p/KCNT2 axis will help us to understand the role of HAND2-AS1 in cancer.

<p>LncRNA CTBP1-AS2 Facilitates Gastric Cancer Progression via Regulating the miR-139-3p/MMP11 Axis</p>

“… 24 LncRNA FTX expression in GC is also up-regulated, and FTX can promote GC proliferation and invasion by adsorbing miR-144 and up-regulating ZFX ; 25 conversely, as a tumor suppressor, lncRNA HAND2-AS1 inhibits the proliferation and migration of GC cells through modulating miR-590-3p/ KCNT2 axis. 26 In this study, we verified that CTBP1-AS2 was a molecular sponge for miR-139-3p. Furthermore, functional experiments illustrated that CTBP1-AS2 promoted cell proliferation and suppressed apoptosis by inhibiting miR-139-3p expression.…”

Long non-coding RNA HAND2-AS1 inhibits gastric cancer progression by suppressing TCEAL7 expression via targeting miR-769–5p

“…In GC, TCEAL7 was revealed to be decreased expression and correlated with prognosis of cancer patients [20] . Our previous work suggested that HAND2-AS1 could regulate GC cell growth and migration via regulating the miR-590-3p/KCNT2 axis [21] . Here, we presented a novel regulatory mechanism for roles of HAND2-AS1 in GC progression.…”

The regulatory role of antisense lncRNAs in cancer

“…Its anti-tumorigenic effect is mediated by downregulating NMU, an oncogenic protein in EEC [ 70 ]. In GC cells, HAND2-AS1 expression is decreased; however, overexpression of HAND2-AS1 is capable of inhibiting GC cell proliferation and promoting their apoptosis by functioning as a ceRNA that binds with miR-590-3p [ 71 ]. Another study demonstrated that HAND2-AS1 can also exert its tumor suppressive role through the miR-769-5p/ TCEAL7 axis in GC [ 72 ].…”