<p>lncRNA NEAT1 Facilitates Cell Proliferation, Invasion and Migration by Regulating CBX7 and RTCB in Breast Cancer</p>

Abstract

To investigate the association between the lncRNA NEAT1 and breast cancer, and to determine the influence of NEAT1 on regulation of other signaling molecules in breast cancer. Methods: In the present study, we measured levels of the lncRNA NEAT1 in 106 breast cancer patients and in a human breast cancer cell line by qRT-PCR. The correlation between NEAT1 expression and patients' clinical characteristics was analyzed with in-house and TCGA data. We used cellular functioning assays and cell immunofluorescence assay to evaluate the role of NEAT1 and its target molecules in proliferation, invasion and migration in breast cancer. We used Western blotting to explore possible targets of NEAT1 and a subcellular fractionation assay to locate NEAT1 expression. Results: NEAT1 was overexpressed in breast cancer tissue and also closely related to advanced clinical stages and positive lymph node metastases. NEAT1 levels were also tightly correlated to prognosis for breast cancer patients in survival analyses. Cellular function assays revealed that downregulation of NEAT1 could inhibit breast cancer cell viability, invasion and migration. Western blotting revealed down-regulation of CBX7 and upregulation of RTCB following NEAT1 inhibition. Based on the cytoplasmic and nuclear expression of NEAT1, we investigated the possible regulation of CBX7 and RTCB by NEAT1. Results showed that NEAT1 regulated the expression of CBX7 and RTCB, possibly by binding of NEAT1 to DNA in the nucleus, which facilitates cell proliferation, invasion and migration. Conclusion: The current results suggest that the lncRNA NEAT1 is upregulated in breast cancer and facilitates tumor cell viability, invasion and migration via CBX7 and RTCB.

Single-cell long noncoding RNA (lncRNA) transcriptome implicates MALAT1 in triple-negative breast cancer (TNBC) resistance to neoadjuvant chemotherapy

“…We suggest a possible reciprocal relationship between MALAT1 and each of AC005154.1, NEAT1, AL139022.1, AC007622.2, USP3-AS1, MUC20-OT1, LINC-PINT, and AC103810.5. In fact, several studies on NEAT1 lncRNA have shown that upregulated NEAT1 facilitated tumor cell viability, invasion, and migration in breast cancer 32 . In Non-small-cell lung carcinoma, Shikonin mediated NEAT1 suppression resulted in apoptotic cell death of paclitaxel-resistant cell lines 33 .…”

<p>Long Non-Coding RNA NEAT1 Promotes the Proliferation, Migration, and Metastasis of Human Breast-Cancer Cells by Inhibiting miR-146b-5p Expression</p>

“…21 lncRNA NEAT1 has been reported to regulate the growth and mobility of BC cells by targeting CBX7 and RTCB. 22 Also, lncRNA NEAT1 confers chemoresistance and cell "stemness" in triple-negative breast cancer (TNBC) cells. 23 Epithelial-mesenchymal transition (EMT) is a key inducer of tumor metastasis, 24 but the effect of lncRNA NEAT1 on EMT in BC cells is not known.…”

CBX7 is Dualistic in Cancer Progression Based on its Function and Molecular Interactions

“…Breast Cancer Bioinformatic analysis of Chromobox proteins family has shown that the most significant difference between breast cancer and normal mammary tissue is the low mRNA expression of CBX7 (Li X. et al, 2020). One study of breast cancer implied that patients with low CBX7 had lower survival and were more likely to develop lymph node metastasis, P53 mutations, and the cancer further deteriorated, metastasized over time (Li X. et al, 2020). The mechanism for its carcinogenicity may be that CBX7 acts as a novel epigenetic regulator of the Wnt/β-catenin pathway in breast cancer to determine cancer progression (Li X. et al, 2020).…”

“…One study of breast cancer implied that patients with low CBX7 had lower survival and were more likely to develop lymph node metastasis, P53 mutations, and the cancer further deteriorated, metastasized over time (Li X. et al, 2020). The mechanism for its carcinogenicity may be that CBX7 acts as a novel epigenetic regulator of the Wnt/β-catenin pathway in breast cancer to determine cancer progression (Li X. et al, 2020). In breast cancer, CBX7 increases Dickkopf-1 (DKK-1, a Wnt antagonist) gene transcription, thus indirectly affecting the Wnt signaling pathway (Bafico et al, 2001;Kim et al, 2015).…”