<p>KIF4A Promotes Clear Cell Renal Cell Carcinoma (ccRCC) Proliferation in vitro and in vivo</p>

Abstract

These authors contributed equally to this workPurpose: To evaluate the expression in human clear cell renal cell carcinoma (ccRCC) tissues and explore the effects of kinesin family member 4A (KIF4A) on ccRCC progression. Methods: GEPIA was used to evaluate the mRNA levels of KIF4A in human ccRCC tissues from TCGA database, and Immunohistochemistry (IHC) assays were performed to assess its expression in human ccRCC tissues collected in our hospital. The clinical-pathological analysis was performed to explore the correlation with KIF4A expression. The effects of KIF4A on ccRCC cell proliferation were detected through colony formation and MTT assays. Finally, the effects of KIF4A on tumor growth were measured using a mice model. Results: Bioinformation results showed the expression of KIF4A mRNA was upregulated in ccRCC tissues and high expression of KIF4A was related with poor prognosis in ccRCC patients. We also found a high expression of KIF4A in human ccRCC tissues collected in our hospital. We also found its expression level was correlated with clinical characteristics, including T stage (P=0.035*) and lymphatic metastasis (P=0.028*). We further confirmed that knockdown of KIF4A suppressed cell proliferation in HTB-47 and CRL-1932 cells. Furthermore, KIF4A contributes to tumor growth of ccRCC cells in mice. Conclusion: We found the abnormal high expression of KIF4A in human ccRCC tissues and demonstrated that KIF4A could serve as a tumor induction gene.

KIF4A: A potential biomarker for prediction and prognostic of prostate cancer

“…* P＜0.01. KIF4A expression is involved in multiple human cancer, including hepatocellular carcinoma, glioblastoma, oral cancer, lung cancer, breast cancer, colorectal cancer and clear cell renal cell carcinoma [29][30][31][32][33]. Consistent with previous observations, enhancive expression of KIF4A has been observed in 71% of PCa patients compared with healthy samples [36].…”

“…In addition, we used GEPIA online tool to analyze KIF4A expressions in other malignancies, and found that the relative KIF4A mRNA levels were higher in specific tumors, compared with normal tissue. Recent studies have demonstrated that abnormal KIF4A expression is involved in the progressions of multiple human cancers [15,17,18,20,32,33]. These results support the suggestion that KIF4A may serve as a general oncogene.…”

Let-7i-5p enhances cell proliferation, migration and invasion of ccRCC by targeting HABP4

“…Given that most patients with ccRCC are not sensitive to chemotherapy or radiation, surgical treatment has remained most effective [ 5 , 6 ]. Thus, exploring the molecular mechanisms underlying the development of ccRCC and identifying effective treatment strategies to improve the survival rate of patients with ccRCC hold considerable clinical value.…”

ERCC6L facilitates the progression of laryngeal squamous cell carcinoma by the binding of FOXM1 and KIF4A

“…KIF4A is overexpressed in most tumors, but also low in a minority [ 20 ]. Yang et al, found that KIF4A is abnormally highly expressed in human clear cell renal cell carcinoma tissues and can act as a tumor-inducing gene [ 21 ]. Zhu et al, proposed that enhanced KIF4A expression in osteosarcoma predicts poor prognosis and promotes tumor growth by activating the MAPK pathway [ 22 ].…”