<p>HOXA10 deteriorates gastric cancer through activating JAK1/STAT3 signaling pathway</p>

Abstract

Background: HOXA10 has been reported to be deregulated in many kinds of cancers including gastric cancer. But its role in gastric cancer progression is controversial. Therefore, the current study was performed to explore the role and mechanism of HOXA10 in gastric cancer. Materials and methods: IHC and Western blotting assays were used to assess HOXA10 expression in gastric cancer tissues and cells. Lentivirus infection was used to alter HOXA10, STAT3 and JAK1 expression in gastric cancer NCI-N87 and MKN28 cells. MTT, cloning formation, flow cytometry and in vivo xenotransplantation experiments were carried out to assess cell proliferation, cloning formation, apoptosis and tumorigenesis. Results: HOXA10 expression was obviously increased in gastric cancer tissues and cells when compared with the normal gastric tissue samples and cells. Upregulation of HOXA10 significantly enhanced cell proliferation, cloning formation and tumorigenesis abilities and reduced cell apoptosis in gastric cancer, and promoted the activation of JAK1/STAT3 signaling. In addition, we showed that the effects of HOXA10 on the promotion of cell viability and tumorigenesis and cell apoptosis repression were all weakened when JAK1 or STAT3 was downregulated. Conclusion: This study demonstrates that HOXA10 functions as an oncogene in gastric cancer through activating JAK1/STAT3 signaling.

Pan-Cancer Genome-Wide DNA Methylation Analyses Revealed That Hypermethylation Influences 3D Architecture and Gene Expression Dysregulation in HOXA Locus During Carcinogenesis of Cancers

“…Similarly, the role of HOXA4 gene expression during carcinogenesis, prognosis, and drug resistance is also different among cancers ( Bhatlekar et al, 2018 ; Miller et al, 2018 ; Tang et al, 2019 ). The gene expressions and functions of HOXA5 ( Ordóñez-Morán et al, 2015 ; Zhang et al, 2017 ; Peng et al, 2018 ), HOXA6 ( Wu et al, 2018 , 2019 ), HOXA7 ( Tang et al, 2016 ), HOXA9 ( Fu et al, 2017 ; de Bock et al, 2018 ), HOXA10 ( Chen et al, 2019 ; Hatanaka et al, 2019 ), and HOXA11 ( Zhang R. et al, 2018 ) are also controversial across cancers, except for HOXA13 ( He et al, 2017 ). Specifically, 3′ non-coding HOXA mutation was detected in acute myeloid leukemia ( Akdemir et al, 2020 ).…”

<p>MiRNA-128 and MiRNA-142 Regulate Tumorigenesis and EMT in Oral Squamous Cell Carcinoma Through HOXA10</p>

“…It is widely acknowledged that miRNA could exert its function by interacting with the target mRNAs. 32 For example, miR-128 directly targeted vascular endothelial growth factor (VEGF)-C and impeded cell proliferation in non-small cell lung cancer (NSCLC). 33 MiR-142 suppressed cervical cancer progression by targeting high-mobility group box 1 protein (HMGB1).…”

Extract of Stellera Chamaejasme L. Inhibits the Progression of Hepatocellular Carcinoma by Regulating miR-134-5p and JAK1/STAT3 Pathway

“…Chen et al found that HOXA10 deteriorated gastric cancer through activating JAK1/STAT3 pathway. 28 Zhou et al reported that CtBPs promoted the malignancy of osteosarcoma cells through JAK1/STAT3 pathway. 29 From the results above, the authors knew that ESC regulated the expression of miR-134-5p and miR-134-5p could bind to the 3¢UTR of JAK1, which inspired the curiosity to investigate whether ESC and miR-134-5p were involved in JAK1-STAT3 pathway.…”