&lt;p&gt;Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms&lt;/p&gt;

Chen, Haoxiang; Zhong, Juchang; Wang, Jian; Huang, Ruiying; Qiao, Xiaoyin; Wang, Honghui; Tan, Zhikai

doi:10.2147/ijn.s193624

Cited by 27 publications

(26 citation statements)

References 53 publications

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“…In order to mimic the extracellular environment and the native cellular morphology, the main bioengineering strategy is focused on the 3D encapsulation of muscular cell precursors in biocompatible materials. In the last years, 3D bioprinting, 43 – 54 hydrogel molding, 55 – 59 and microporous scaffolds 60 – 62 have been implemented to fabricate skeletal muscle tissues.…”

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

“…To mimic the extracellular matrix, poly lactic-co-glycolic acid (PLGA) multilayered scaffolds were made with E-jet 3D printing. 53 By comparing different fibrilar gaps in the scaffolds, the authors concluded that 50 µm gaps enhance cell adhesion and proliferation. In all the previous works, the bioprinting design was based on lines or meshes to guide the alignment of myotubes.…”

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

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Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

et al. 2021

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Muscular dystrophies are a group of highly disabling disorders that share degenerative muscle weakness and wasting as common symptoms. To date, there is not an effective cure for these diseases. In the last years, bioengineered tissues have emerged as powerful tools for preclinical studies. In this review, we summarize the recent technological advances in skeletal muscle tissue engineering. We identify several ground-breaking techniques to fabricate in vitro bioartificial muscles. Accumulating evidence shows that scaffold-based tissue engineering provides topographical cues that enhance the viability and maturation of skeletal muscle. Functional bioartificial muscles have been developed using human myoblasts. These tissues accurately responded to electrical and biological stimulation. Moreover, advanced drug screening tools can be fabricated integrating these tissues in electrical stimulation platforms. However, more work introducing patient-derived cells and integrating these tissues in microdevices is needed to promote the clinical translation of bioengineered skeletal muscle as preclinical tools for muscular dystrophies.

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Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

Section: D Engineering For Skeletal Muscle Culturementioning

confidence: 99%

Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

et al. 2021

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“…3D printed scaffolds with defined mechanical properties have been designed to guide cellular growth and differentiation, [67] and substrate topography can stimulate actin filament alignment within single cells [68] and enhance tissue maturation. [69] While it has been shown that both the rigidity and the shape of the ECM influences cell development, both aspects have not yet been optimized within the same in vitro assay.…”

Section: Tunable High-precision Scaffolds For Myoblast Actin Fiber Almentioning

confidence: 99%

Precision 3D‐Printed Cell Scaffolds Mimicking Native Tissue Composition and Mechanics

Erben

Hartmann

Becke

et al. 2020

Adv Healthcare Materials

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Cellular dynamics are modeled by the 3D architecture and mechanics of the extracellular matrix (ECM) and vice versa. These bidirectional cell‐ECM interactions are the basis for all vital tissues, many of which have been investigated in 2D environments over the last decades. Experimental approaches to mimic in vivo cell niches in 3D with the highest biological conformity and resolution can enable new insights into these cell‐ECM interactions including proliferation, differentiation, migration, and invasion assays. Here, two‐photon stereolithography is adopted to print up to mm‐sized high‐precision 3D cell scaffolds at micrometer resolution with defined mechanical properties from protein‐based resins, such as bovine serum albumin or gelatin methacryloyl. By modifying the manufacturing process including two‐pass printing or post‐print crosslinking, high precision scaffolds with varying Young's moduli ranging from 7‐300 kPa are printed and quantified through atomic force microscopy. The impact of varying scaffold topographies on the dynamics of colonizing cells is observed using mouse myoblast cells and a 3D‐lung microtissue replica colonized with primary human lung fibroblast. This approach will allow for a systematic investigation of single‐cell and tissue dynamics in response to defined mechanical and bio‐molecular cues and is ultimately scalable to full organs.

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“…The spatial arrangement, porosity, biocompatibility, and proper scale of the ECM are some of the most important features that must be adjusted for use in nervous tissue, skin, bone, and muscle [ 76 ]. Nevertheless, several other factors, such as mechanical properties and chemical modification of scaffolds, significantly influence cellular behavior [ 5 , 87 ].…”

Section: Polysaccharide-based Porous Materials For Tissue Engineermentioning

confidence: 99%

Smart Porous Multi-Stimulus Polysaccharide-Based Biomaterials for Tissue Engineering

2020

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Recently, tissue engineering and regenerative medicine studies have evaluated smart biomaterials as implantable scaffolds and their interaction with cells for biomedical applications. Porous materials have been used in tissue engineering as synthetic extracellular matrices, promoting the attachment and migration of host cells to induce the in vitro regeneration of different tissues. Biomimetic 3D scaffold systems allow control over biophysical and biochemical cues, modulating the extracellular environment through mechanical, electrical, and biochemical stimulation of cells, driving their molecular reprogramming. In this review, first we outline the main advantages of using polysaccharides as raw materials for porous scaffolds, as well as the most common processing pathways to obtain the adequate textural properties, allowing the integration and attachment of cells. The second approach focuses on the tunable characteristics of the synthetic matrix, emphasizing the effect of their mechanical properties and the modification with conducting polymers in the cell response. The use and influence of polysaccharide-based porous materials as drug delivery systems for biochemical stimulation of cells is also described. Overall, engineered biomaterials are proposed as an effective strategy to improve in vitro tissue regeneration and future research directions of modified polysaccharide-based materials in the biomedical field are suggested.

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<p>Enhanced growth and differentiation of myoblast cells grown on E-jet 3D printed platforms</p>

Cited by 27 publications

References 53 publications

Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

Bioengineeredin vitroskeletal muscles as new tools for muscular dystrophies preclinical studies

Precision 3D‐Printed Cell Scaffolds Mimicking Native Tissue Composition and Mechanics

Smart Porous Multi-Stimulus Polysaccharide-Based Biomaterials for Tissue Engineering

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