&lt;p&gt;Enhanced Antibacterial Activity of Silver Nanoparticles Combined with Hydrogen Peroxide Against Multidrug-Resistant Pathogens Isolated from Dairy Farms and Beef Slaughterhouses in Egypt&lt;/p&gt;

El-Gohary, Fatma A.; Abdel-Hafez, Lina Jamil M; Zakaria, Amira; Shata, Radwa Reda; Tahoun, Amin; El‐Mleeh, Amany; Elfadl, Eman A. Abo; Elmahallawy, Ehab Kotb

doi:10.2147/idr.s271261

Cited by 18 publications

(10 citation statements)

References 109 publications

(125 reference statements)

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“…In this study, the Salmonella isolation rate of the examined abattoir floors (30%) was greater than those informed in previous works, including 13.33% in Sokoto abattoir, Nigeria [ 36 ]; 18% in Hawassa, Southern Ethiopia [ 37 ]; and 6.3% in Abuja abattoirs, Nigeria [ 38 ]. Furthermore, Salmonella species were recovered from 25% of abattoir walls, which is comparable to El-Gohary et al [ 39 ], who recovered Salmonella isolates from abattoir walls with a prevalence of 30%. In contrast, some studies revealed that Salmonella could not be detected from abattoir walls [ 40 ].…”

Section: Discussionsupporting

confidence: 79%

Anti-Biofilms’ Activity of Garlic and Thyme Essential Oils against Salmonella typhimurium

et al. 2022

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Biofilm control by essential oil (EO) application has recently increased to preclude biofilm production on foods and environmental surfaces. In this work, the anti-biofilm effects of garlic and thyme essential oils using the minimum inhibitory concentration (MIC) method against Salmonella typhimurium recovered from different abattoir samples were investigated along with the virulence genes (InvA, SdiA and Stn genes), and the antimicrobial susceptibility profile of S. typhimurium as well. The obtained results revealed that S. typhimurium contaminated abattoir samples to varying degrees. The InvA gene was investigated in all isolates, whereas the SdiA and Stn genes were observed in four and three isolates, respectively. Utilizing the disc diffusion method, S. typhimurium isolates demonstrated substantial resistance to most of the examined antibiotics with a high multiple antibiotic resistance index. S. typhimurium isolates demonstrated biofilm formation abilities to various degrees at varied temperatures levels (4 °C and 37 °C). In conclusion, the obtained samples from the research area are regarded as a potential S. typhimurium contamination source. Furthermore, garlic essential oil (GEO) has more potential to inhibit S. typhimurium biofilm at different sub-minimum inhibitory concentrations as compared to thyme essential oil (TEO). Therefore, these EOs are considered as potential natural antibacterial options that could be applied in food industry.

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Section: Discussionsupporting

confidence: 79%

Anti-Biofilms’ Activity of Garlic and Thyme Essential Oils against Salmonella typhimurium

et al. 2022

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“…Furthermore, there are different factors that make AgNPs suitable for use as antimicrobial: (1) It is simple and safe to synthesize on a large scale with low cost [ 16 ]; (2) bacterial resistance to AgNPs is extremely rare; and (3) the surface of AgNPs can be easily modified and synthesized in various shapes [ 17 ]. Several studies have reported that AgNPs have antimicrobial activity against a variety of pathogens, including Salmonella enterica, E. coli O157:H7, Pseudomonas aeruginosa, Listeria monocytogenes, Klebsiella pneumoniae [ 18 ], and mastitis pathogens [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance of Salmonella enteritidis and Salmonella typhimurium Isolated from Laying Hens, Table Eggs, and Humans with Respect to Antimicrobial Activity of Biosynthesized Silver Nanoparticles

Elez

Elsohaby

El‐Gazzar

et al. 2021

Animals

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Salmonella enterica is one of the most common causes of foodborne illness worldwide. Contaminated poultry products, especially meat and eggs are the main sources of human salmonellosis. Thus, the aim of the present study was to determine prevalence, antimicrobial resistance profiles, virulence, and resistance genes of Salmonella Enteritidis (S. enteritidis) and Salmonella Typhimurium (S. Typhimurium) isolated from laying hens, table eggs, and humans, in Sharkia Governorate, Egypt. The antimicrobial activity of Biosynthesized Silver Nanoparticles (AgNPs) was also evaluated. Salmonella spp. were found in 19.3% of tested samples with laying hens having the highest isolation rate (33.1%). S. Enteritidis) (5.8%), and S. Typhimurium (2.8%) were the dominant serotypes. All isolates were ampicillin resistant (100%); however, none of the isolates were meropenem resistant. Multidrug-resistant (MDR) was detected in 83.8% of the isolates with a multiple antibiotic resistance index of 0.21 to 0.57. Most isolates (81.1%) had at least three virulence genes (sopB, stn, and hilA) and none of the isolates harbored the pefA gene; four resistance genes (blaTEM, tetA, nfsA, and nfsB) were detected in 56.8% of the examined isolates. The AgNPs biosynthesized by Aspergillus niveus exhibit an absorption peak at 420 nm with an average size of 27 nm. AgNPs had a minimum inhibitory concentration of 5 µg/mL against S. enteritidis and S. typhimurium isolates and a minimum bactericidal concentration of 6 and 8 µg/mL against S. enteritidis and S. typhimurium isolates, respectively. The bacterial growth and gene expression of S. enteritidis and S. typhimurium isolates treated with AgNPs were gradually decreased as storage time was increased. In conclusion, this study indicates that S. enteritidis and S. typhimurium isolated from laying hens, table eggs, and humans exhibits resistance to multiple antimicrobial classes. The biosynthesized AgNPs showed potential antimicrobial activity against MDR S. enteritidis and S. typhimurium isolates. However, studies to assess the antimicrobial effectiveness of the biosynthesized AgNPs in laying hen farms are warranted.

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“…The use of NPs in inhibiting biofilms has been previously documented for various bacteria [ 79 , 80 , 81 ]. This study indicated the potential of AgNPs to inhibit biofilm production at concentrations of 75 and 100 µg/mL, and the percentage of inhibition was 80.8–82.8% at the highest AgNP concentrations.…”

Section: Discussionmentioning

confidence: 99%

Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers

Ahmed

Bayomi

Hamed³

et al. 2022

Veterinary Sciences

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In this study, we determined the prevalence and toxin types of antibiotic-resistant Clostridium perfringens in chicken, pigeons, camels, and humans. We investigated the inhibitory effects of AgNPs on biofilm formation ability of the isolates and the genetic relatedness of the isolates from various sources determined using RAPD-PCR. Fifty isolates were identified using PCR, and all the isolates were of type A. The cpe and cpb2 genes were detected in 12% and 56% of the isolates, respectively. The effect of AgNPs on biofilm production of six representative isolates indicated that at the highest concentration of AgNPs (100 µg/mL), the inhibition percentages were 80.8–82.8%. The RAPD-PCR patterns of the 50 C. perfringens isolates from various sources revealed 33 profiles and four clusters, and the discriminatory power of RAPD-PCR was high. Multidrug-resistant C. perfringens isolates are predominant in the study area. The inhibition of biofilm formation by C. perfringens isolates was dose-dependent, and RAPD-PCR is a promising method for studying the genetic relatedness between the isolates from various sources. This is the first report of AgNPs’ anti-biofilm activity against C. perfringens from chickens, pigeons, camels, and humans, to the best of our knowledge.

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<p>Enhanced Antibacterial Activity of Silver Nanoparticles Combined with Hydrogen Peroxide Against Multidrug-Resistant Pathogens Isolated from Dairy Farms and Beef Slaughterhouses in Egypt</p>

Cited by 18 publications

References 109 publications

Anti-Biofilms’ Activity of Garlic and Thyme Essential Oils against Salmonella typhimurium

Anti-Biofilms’ Activity of Garlic and Thyme Essential Oils against Salmonella typhimurium

Antimicrobial Resistance of Salmonella enteritidis and Salmonella typhimurium Isolated from Laying Hens, Table Eggs, and Humans with Respect to Antimicrobial Activity of Biosynthesized Silver Nanoparticles

Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers

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