&lt;p&gt;Distinct Antimicrobial Resistance Profiling Of Clinically Important &lt;em&gt;Aeromonas&lt;/em&gt; Spp. In Southwest China: A Seven-Year Surveillance Study&lt;/p&gt;

Ss, Yang; He, Tong Chuan; Jin, Sun; Sun, Shujuan

doi:10.2147/idr.s216926

Cited by 9 publications

(7 citation statements)

References 35 publications

(36 reference statements)

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“…Moreover, Aeromonas salmonicida spp salmonicida is resistant to some β-lactam antibiotics such as Cefixime (CFM), Cefrtriaxone (CRO), Cefoxitin (FOX), Piperacillin (PRL), and the cephalosporin family (Table 4). This resistance is similar with the results of a study conducted by Valdes et al [2015)] and Yang et al [2019]. However, it is sensitive to Aminoside, Penicillin, Phenicole, Furoquinolonen, Macrolide, Fusidamine, Lincosamide, Quinolone, Rifamycin, Sulfamide, Cycline, Glycopeptide and other antibiotics from β-lactamine family as Cefalexin (CN), Cefotetan (CT), Cefotaxim (CTX), Imipenem (IPM).…”

Section: Assessment Of Antibiotic Resistancesupporting

confidence: 88%

Network Water Quality at a Hospital Center in Morocco: Bacteriological Survey and Relationship with Human Health

Flouchi¹,

Elmniai²,

Abbou³

et al. 2021

J. Ecol. Eng.

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Water is mandatory for the functioning of hospitals. Its consumption varies from one service to another, and its use can reduce the service quality on one hand and presents a major risk of nosocomial infection on the other hand. The health risks related to the use of water in hospitals are mostly microbiological, but sometimes they can be chemical. For this reason, the conducted work aimed to evaluate, for the first time, the quality and bacteriological efficiency of the network water of the provincial hospital center IBN BAJA in Taza, Northeast Morocco. During one year, 72 samples were analyzed in six surgery departments, one sample per month for each ward at a rate of six samples per month and per department. The results obtained showed that the water quality of the hospital network was in conformity with the requirements of the Moroccan standards. Nonetheless, some bacterial strains such as Aeromonas salmonicida spp salmonicida, Enterococcus spp., Pseudomonas luteola, Sphingomonas paucimobilis, Pseudomonas stutzeri, Stenotrophonomas maltophilia, Burkholderia cepacia and Micrococcus luteus, which constitute a major risk to human health, were found. Moreover, after sensitivity evaluation to the twenty-seven antibiotics, some strains have been shown to be multi-resistant, which can present a major risk of nosocomial infections in the studied hospital for human beings.

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Section: Assessment Of Antibiotic Resistancesupporting

confidence: 88%

Network Water Quality at a Hospital Center in Morocco: Bacteriological Survey and Relationship with Human Health

Flouchi¹,

Elmniai²,

Abbou³

et al. 2021

J. Ecol. Eng.

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“…in Southwest China from 2011 to 2017. 26 And this report also mentioned that A. hydrophila has a resistance rates of 30.2% to ceftriaxone; 20% to ceftazidime, cefepime, aztreonam, and ciprofloxacin; and less than 10% to imipenem and meropenem. These findings suggest that the drug resistance of A. hydrophila is an increasingly serious problem in clinical infections.…”

Section: Discussionmentioning

confidence: 78%

Molecular and Functional Characterization of a Novel Plasmid-Borne blaNDM-Like Gene, blaAFM-1, in a Clinical Strain of Aeromonas hydrophila

Lin¹,

Lü²,

Qian³

et al. 2021

IDR

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Purpose: An increasing frequency of antibiotic resistance has been observed in both clinical and environmental Aeromonas hydrophila isolates in recent years. However, there are still very few in-depth studies regarding the role of plasmids in the antibiotic resistance of A. hydrophila. Hence, we investigated the molecular and functional characterization of a multidrug-resistant plasmid encoding an NDM-like metallo-β-lactamase, AFM-1, in the clinical A. hydrophila isolate SS332. Methods: The minimum inhibitory concentrations (MICs) of 24 antibiotics against A. hydrophila SS332 were measured by the agar dilution method. The genome of A. hydrophila SS332 was sequenced with PacBio and Illumina platforms. Six plasmidborne antimicrobial resistance genes were chosen for cloning, including bla AFM-1 , bla OXA-1 , msr(E), mph(E), aac(6ʹ)-Ib10, and aph(3ʹ)-Ia. Phylogenetic analysis, amino acid sequence alignment, and comparative genomic analysis were performed to elucidate the active site requirements and genetic context of the bla AFM-1 gene. Results: A. hydrophila SS332 showed high levels of resistance to 15 antibiotics, especially those with MIC levels at or above 1024 μg/mL, including ampicillin, cefazolin, ceftriaxone, aztreonam, spectinomycin, and roxithromycin. Six plasmid-borne resistance genes from A. hydrophila were verified to be functional in E. coli DH5α. AFM-1 shared 86% amino acid identity with NDM-1 and showed resistance to ampicillin, cefazolin, cefoxitin, and ceftazidime. In addition, the bla AFM-1 gene was associated with three different novel ISCR19-like elements, designated ISCR19-1, ISCR19-2 and ∆ISCR19-3, which may be involved in the acquisition and mobilization of the bla AFM-1 gene. Conclusion: Our investigation showed that plasmid-borne resistance genes can contribute to antibiotic resistance in A. hydrophila SS332. A novel bla NDM -like gene, bla AFM-1 , was verified to be functional and associated with novel ISCR19-like elements. This fact indicated the risk of spread of bla AFM-1 genes and ISCR19-like elements.

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“…A previous seven-year surveillance study demonstrated that A. hydrophila (65.6%, 745/1135) was the most predominant species from clinical Aeromonas spp. in Southwest China from 2011 to 2017 [21]. It also reported that A. hydrophila has a resistance rates of 30.2% to ceftriaxone, followed by 20% to ceftazidime, cefepime, aztreonam, and cipro oxacin, and less than 10% to imipenem and meropenem.…”

Section: Discussionmentioning

confidence: 97%

Molecular and Functional Characterization of a Novel Plasmid-Borne blaNDM variant, blaAFM-1, in a Clinical Strain of Aeromonas Hydrophila

Lin

Qian

et al. 2020

Preprint

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BackgroundAeromonas hydrophila is a zoonotic and human opportunistic pathogen. Increasing antibiotic resistance profiles have been discovered in both clinical and environmental A. hydrophila isolates in recent years. However, there are still very few in-depth studies regarding the role of plasmids in antibiotic resistance of A. hydrophila. Hence, we investigate the molecular characterization and functional characterization of a multidrug resistant plasmid which encoding a novel NDM variant, AFM-1, in clinical A. hydrophila SS332.MethodsThe minimum inhibitory concentrations (MICs) of 24 antibiotics for A. hydrophila SS332 were measured by the agar dilution method. The genome of A. hydrophila SS332 was sequenced with Pacific and Illumina platforms. Six plasmid-borne antimicrobial resistance genes were chosen for cloning, including blaAFM-1, blaOXA-1, msr(E), mph(E), aac(6')-Ib10, and aph(3')-Ia. Phylogenetic analysis, amino acid sequence alignment, and comparative genomic analysis were performed to elucidate the active site requirements and genetic context of blaAFM-1 gene.ResultsA. hydrophila SS332 showed high levels of resistance to 15 antibiotics, especially those with MIC levels up to or higher than 1024 μg/mL, including ampicillin, cefazolin, ceftriaxone, aztreonam, spectinomycin, roxithromycin. Six plasmid-borne resistance genes were verified to be functional in E. coli DH5α. The blaAFM-1 gene, a novel blaNDM variant, encodes a subclass B1 metallo-β-lactamase AFM-1 which shares 86% amino acid identity with NDM-1. AFM-1 showed resistance to penicillins, cephalosporins and carbapenems. Besides, blaAFM-1 gene was associated with three different novel ISCR19-like elements, designated ISCR19-1, ISCR19-2 and ∆ISCR19-3, which may be involved in the acquisition and mobilization of blaAFM-1 gene. ConclusionsOur investigation showed that plasmid-borne resistance genes played a major role in the resistance profiles of A. hydrophila SS332. A novel blaNDM variant, blaAFM-1, was verified to be functional and disseminated among different bacteria species mediated by novel ISCR19-like elements. The fact indicated that the risk of spread of novel resistance genes and novel ISCR elements.

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<p>Distinct Antimicrobial Resistance Profiling Of Clinically Important <em>Aeromonas</em> Spp. In Southwest China: A Seven-Year Surveillance Study</p>

Cited by 9 publications

References 35 publications

Network Water Quality at a Hospital Center in Morocco: Bacteriological Survey and Relationship with Human Health

Network Water Quality at a Hospital Center in Morocco: Bacteriological Survey and Relationship with Human Health

Molecular and Functional Characterization of a Novel Plasmid-Borne blaNDM-Like Gene, blaAFM-1, in a Clinical Strain of Aeromonas hydrophila

Molecular and Functional Characterization of a Novel Plasmid-Borne blaNDM variant, blaAFM-1, in a Clinical Strain of Aeromonas Hydrophila

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