&lt;p&gt;Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma&lt;/p&gt;

Chiu, Nan Fu; Tai, Ming Jung; Wu, Hwai Ping; Lin, Ting; Chen, Chen-Yu

doi:10.2147/ijn.s213653

Cited by 17 publications

(20 citation statements)

References 41 publications

(43 reference statements)

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“…The work function was calculated as Φ = hν-E C -E F , where hν is the incident photon energy (21.2 eV). 23 , 51 , 52 The MoS 2 and carboxyl-MoS 2 sheets were separately immobilized on BK7 substrates coated with 47 nm of Au film. As shown in Figure 4C , the binding energies of Au, Au/MoS 2 and Au/carboxyl-MoS 2 in the layer-by-layer material surface were 16.1, 17.0 and 16.5 eV, respectively.…”

Section: Resultsmentioning

confidence: 99%

“… 22 In previous work, we showed that an SPR immunosensor based on functionalized graphene oxide (GO) could be used to detect PAPP-A2 at levels as low as 0.01 pg/mL in spiked human plasma. 23 In addition, Fan et al developed a GO-based SPR biosensor that allowed for the label-free simultaneous detection of PAPP-A and PAPP-A2 in clinical blood samples during pregnancy. 24 In addition, we demonstrated that a carboxyl-GO-based SPR aptasensor to assay hCG in the blood serum of pregnant women had a detection limit of 1.15 pM, 25 and that it could be used to screen women with fetal DS by detecting hCG protein at limits of 1 pM in buffer samples and 1.9 pM in clinical serum samples.…”

Section: Introductionmentioning

confidence: 99%

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Immunoassay-Amplified Responses Using a Functionalized MoS2-Based SPR Biosensor to Detect PAPP-A2 in Maternal Serum Samples to Screen for Fetal Down’s Syndrome

Chiu¹,

Tai²,

Nurrohman

et al. 2021

IJN

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Background Due to educational, social and economic reasons, more and more women are delaying childbirth. However, advanced maternal age is associated with several adverse pregnancy outcomes, and in particular a high risk of Down’s syndrome (DS). Hence, it is increasingly important to be able to detect fetal Down’s syndrome (FDS). Methods We developed an effective, highly sensitive, surface plasmon resonance (SPR) biosensor with biochemically amplified responses using carboxyl-molybdenum disulfide (MoS 2 ) film. The use of carboxylic acid as a surface modifier of MoS 2 promoted dispersion and formed specific three-dimensional coordination sites. The carboxylic acid immobilized unmodified antibodies in a way that enhanced the bioaffinity of MoS 2 and preserved biorecognition properties of the SPR sensor surface. Complete antigen pregnancy-associated plasma protein-A2 (PAPP-A2) conjugated with the carboxyl-MoS 2 -modified gold chip to amplify the signal and improve detection sensitivity. This heterostructure interface had a high work function, and thus improved the efficiency of the electric field energy of the surface plasmon. These results provide evidence that the interface electric field improved performance of the SPR biosensor. Results The carboxyl-MoS 2 -based SPR biosensor was used successfully to evaluate PAPP-A2 level for fetal Down’s syndrome screening in maternal serum samples. The detection limit was 0.05 pg/mL, and the linear working range was 0.1 to 1100 pg/mL. The women with an SPR angle >46.57 m° were more closely associated with fetal Down’s syndrome. Once optimized for serum Down’s syndrome screening, an average recovery of 95.2% and relative standard deviation of 8.5% were obtained. Our findings suggest that carboxyl-MoS 2 -based SPR technology may have advantages over conventional ELISA in certain situations. Conclusion Carboxyl-MoS 2 -based SPR biosensors can be used as a new diagnostic technology to respond to the increasing need for fetal Down’s syndrome screening in maternal serum samples. Our results demonstrated that the carboxyl-MoS 2 -based SPR biosensor was capable of determining PAPP-A2 levels with acceptable accuracy and recovery. We hope that this technology will be investigated in diverse clinical trials and in real case applications for screening and early diagnosis in the future.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunoassay-Amplified Responses Using a Functionalized MoS2-Based SPR Biosensor to Detect PAPP-A2 in Maternal Serum Samples to Screen for Fetal Down’s Syndrome

Chiu¹,

Tai²,

Nurrohman

et al. 2021

IJN

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“…In 2019, Chiu et al ( 2019b ) developed a carboxyl-GO–based SPR immunosensor to detect pregnancy protein-associated plasma protein A2 (PAPP-A2) in human blood plasma. The carboxyl-GO surface was functionalized by utilizing covalent bonds between carboxylic acid and anti–PAPP-A2 protein.…”

Section: Graphene-based Spr Biosensormentioning

confidence: 99%

“…(B) Calibration curve of the average SPR response to various PAPP-A2 protein concentrations. Figures (A,B) were reproduced from Chiu et al ( 2019b ) with permission from the Dove Medical Press.…”

Section: Graphene-based Spr Biosensormentioning

confidence: 99%

Exploring Graphene and MoS2 Chips Based Surface Plasmon Resonance Biosensors for Diagnostic Applications

2020

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Until now, two-dimensional (2D) nanomaterials have been widely studied and applied in the biosensor field. Some of the advantages offered by these 2D materials include large specific surface area, high conductivity, and easy surface modification. This review discusses the use of 2D material in surface plasmon resonance (SPR) biosensor for diagnostic applications. Two-dimensional material reviewed includes graphene and molybdenum disulfide (MoS 2 ). The discussion begins with a brief introduction to the general principles of the SPR biosensor. The discussion continues by explaining the properties and characteristics of each material and its effect on the performance of the SPR biosensor, in particular its sensitivity. This review concludes with some recent applications of graphene- and MoS 2 -based SPR biosensor in diagnostic applications.

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“…In addition, we previously reported the development of an aptasensor using carboxyl-GO to detect human chorionic gonadotropin (hCG) protein in clinical serum samples (Chiu et al, 2019a). Also, an immunosensor by using carboxyl-GO to detect pregnancy-associated plasma protein A2 in human plasma (Chiu et al, 2019b). The main advantages of these graphene-like nanocomposite materials are their unique functional group structures, ease of synthesis, cost-effectiveness, excellent bioaffinity, sensitivity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

High-Sensitivity Detection of the Lung Cancer Biomarker CYFRA21-1 in Serum Samples Using a Carboxyl-MoS2 Functional Film for SPR-Based Immunosensors

Chiu

Yang

2020

Front. Bioeng. Biotechnol.

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We constructed a novel surface plasmon resonance (SPR) detection assay using carboxyl-functionalized molybdenum disulfide (carboxyl-MoS 2) nanocomposites as a signal amplification sensing film for the ultrasensitive detection of the lung cancer-associated biomarker cytokeratin 19 fragment (CYFRA21-1). The experiment succeeded in MoS 2 reacted with chloroacetic acid giving carboxyl-MoS 2 as the reaction product. The additional shoulder in the C 1s and O 1s peaks of carboxyl-MoS 2 , which were increased in X-ray photoelectron spectroscopy, confirmed the presence of O-C=O groups on the surface of the carboxyl-MoS 2. Compared to MoS 2 , the experimental results confirmed that carboxyl-modified MoS 2 had improved low impedance and low refractive index. The carboxyl-MoS 2-based chip had a high affinity, with an SPR angle shift enhanced by 2.6-fold and affinity binding K A enhanced by 15-fold compared to a traditional SPR sensor. The results revealed that the carboxyl-MoS 2-based chip had high sensitivity, specificity, and SPR signal affinity, while the CYFRA21-1 assay in spiked clinical serum showed a lower detection limit of 0.05 pg/mL and a wider quantitation range (0.05 pg/mL to 100 ng/mL). The carboxyl-MoS 2-based chip detection value was about 10 4 times more sensitive than the limit of detection of an enzyme-linked immunosorbent assay (ELISA) (0.60 ng/mL). The results showed that the carboxyl-MoS 2-based chip had the potential to rapidly assay complex samples including bodily fluids, whole blood, serum, plasma, urine, and saliva in SPR-based immunosensors to diagnose diseases including cancer.

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<p>Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma</p>

Cited by 17 publications

References 41 publications

Immunoassay-Amplified Responses Using a Functionalized MoS2-Based SPR Biosensor to Detect PAPP-A2 in Maternal Serum Samples to Screen for Fetal Down’s Syndrome

Immunoassay-Amplified Responses Using a Functionalized MoS2-Based SPR Biosensor to Detect PAPP-A2 in Maternal Serum Samples to Screen for Fetal Down’s Syndrome

Exploring Graphene and MoS2 Chips Based Surface Plasmon Resonance Biosensors for Diagnostic Applications

High-Sensitivity Detection of the Lung Cancer Biomarker CYFRA21-1 in Serum Samples Using a Carboxyl-MoS2 Functional Film for SPR-Based Immunosensors

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