&lt;p&gt;CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway&lt;/p&gt;

Zhang, Bashan; Li, Fēi; Zhu, Zinian; Ding, Aijiao; Luo, Jintong

doi:10.2147/cmar.s252679

Cited by 27 publications

(7 citation statements)

References 40 publications

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“…Notably, miR-7 can act as a tumor suppressor to induce growth factor signal transduction; therefore, we conclude that circ-CDR1as may regulate the stability of the target gene miR-7 through the sponge action of miRNAs, regulating insulin secretion and tumorigenesis. 39,54,55 These results suggest that circRNAs can act as sponges for miRNAs and participate in the tumorigenesis of CRC, which is expected to be a breakthrough in the diagnosis and treatment of CRC.…”

Section: Crcmentioning

confidence: 90%

Circular RNA: A promising new star for the diagnosis and treatment of colorectal cancer

Zhang

Sun

et al. 2021

Cancer Medicine

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Colorectal cancer (CRC) is one of the most common malignant tumors of the digestive tract, and morbidity and mortality rank third and second in malignant tumors. [1][2][3] Currently, early screening for CRC can effectively improve the survival rate of patients with CRC, but approximately 25% of CRC patients develop metastatic disease from an early stage. [4][5][6] Therefore, a promising field of antimetastatic therapy lies in the targeted inhibition of cancer cells with high metastatic potential from the primary site. 7,8 Recently, there has been a qualitative leap in tumor medical technology, such as the application of laparoscopic surgery, nano-assembly technology, PET/ CT imaging technology, and dynamic network biomarker (DNB), etc. 9-11 However, the cure rate and survival rate of CRC are still very low, mainly lacking new drug treatment targets and biomarkers for the treatment of CRC. 12 According to the research of circular RNAs in the CRC field, circular RNAs are expected to be therapeutic targets

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Section: Crcmentioning

confidence: 90%

Circular RNA: A promising new star for the diagnosis and treatment of colorectal cancer

Zhang

Sun

et al. 2021

Cancer Medicine

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“…RAF1 belongs to the RAF protein kinase family which contains A-RAF, B-RAF and RAF1 (C-RAF), and plays a signal conduction function in MAPK signaling pathway [ 55 ]. It has been reported in a variety of malignancies that RAF1 transmits extracellular signals into the nucleus through cell membrane receptors, thereby mediating the expression of intracellular specific proteins and participating in the regulation of cell proliferation, differentiation, apoptosis and autophagy [ 35 , 56 , 57 ]. In this study, lentivirus knockdown and overexpression of RAF1 were transfected into FADU and SCC15 cell lines, and it was proved that the downregulation of RAF1 could inhibit the proliferation, migration, invasion and promote apoptosis of tumor cells.…”

Section: Discussionmentioning

confidence: 99%

RAF1 promotes lymphatic metastasis of hypopharyngeal carcinoma via regulating LAGE1: an experimental research

Pan

et al. 2022

J Transl Med

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Background Lymphatic metastasis was an independent prognostic risk factor for hypopharyngeal carcinoma and was the main cause of treatment failure. The purpose of this study was to screen the differential genes and investigate the mechanism of lymphatic metastasis in hypopharyngeal carcinoma. Methods Transcriptome sequencing was performed on primary tumors of patients, and differential genes were screened by bioinformatics analysis. The expression of differential genes was verified by qRT-PCR, western-blotting and immunohistochemical, and prognostic value was analyzed by Kaplan–Meier and log-rank test and Cox’s test. Next, FADU and SCC15 cell lines were used to demonstrate the function of differential genes both in vitro by EdU, Flow cytometry, Wound Healing and Transwell assays and in vivo by a foot-pad xenograft mice model. Proteomic sequencing was performed to screen relevant targets. In addition, in vitro and in vivo experiments were conducted to verify the mechanism of lymphatic metastasis. Results Results of transcriptome sequencing showed that RAF1 was a significantly differential gene in lymphatic metastasis and was an independent prognostic risk factor. In vitro experiments suggested that decreased expression of RAF1 could inhibit proliferation, migration and invasion of tumor cells and promote apoptosis. In vivo experiments indicated that RAF1 could promote tumor growth and lymphatic metastasis. Proteomic sequencing and subsequent experiments suggested that LAGE1 could promote development of tumor and lymphatic metastasis, and was regulated by RAF1. Conclusions It suggests that RAF1 can promote lymphatic metastasis of hypopharyngeal carcinoma by regulating LAGE1, and provide a basis for the exploring of novel therapeutic target and ultimately provide new guidance for the establishment of intelligent diagnosis and precise treatment of hypopharyngeal carcinoma.

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“…The gene may be involved in insulin secretion from pancreas via exocytosis. MIR1287 is a microRNA that targets RAF1 which is involved in RAS-RAF-MEK-ERK pathways (58); hence, it may influence multiple metabolic process (e.g., glycolysis) relevant to T2DM physiology. MIR1287 was downregulated in symptomatic patients with carotid plaques undergoing carotid endarterectomy (59) suggesting its possible role in metabolic disorders.…”

Section: Discussionmentioning

confidence: 99%

Network analysis after epigenome wide methylation study revealed JUP as a regulator of co-methylated risk-module for T2DM

Prasad

Parekkat

et al. 2021

Preprint

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Controlling the global Type 2 diabetes mellitus (T2DM) pandemic requires a better understanding of its risk factors across different populations, and needs markers that can precisely predict the individual risk to the disease. DNA methylation due to environmental factors is a known mechanism for conferring risk to T2DM. To identify such methylation signatures and associated risk to the disease, we performed an epigenome-wide association study (EWAS) in 844 individuals of Indo-European origin. Within the Indian population, we identified and validated 6 novel differentially methylated CpG sites in PDCD6IP, MIR1287, 5S_rRNA, HDAC9, KCNK16, and RTN1 genes associated with T2DM risk at the epigenome-wide-significance-level (p<1.2x10-7). Further, we also replicated the association of 3 known CpG sites in TXNIP, SREBF1, and CPT1A in the Indian population. Using methylation-based network analysis, we identified 4 co-methylated modules, which we named turquoise, yellow, brown, and blue, among differentially methylated CpG sites in discovery phase samples. We observed that methylation of the brown module with 28 CpG sites, associated with T2DM risk factors (e.g., BMI, insulin, C-peptide). Upon further analysis, we noted that these methylation signatures at 14 of the brown modules CpG can be used as markers to segregate T2DM patients with good glycemic control (e.g., low HbA1c) but poor lipid profile (low HDL and high TG) from the other patients. Additionally, we discovered that rs6503650 in the JUP gene regulates methylation at all the 14 hub CpG sites of the brown modules as methQTL. Our network-assisted epigenome-wide association study is the first to systematically explore DNA methylations conferring risks to T2DM. In addition, the study shows the potential use of identified risk CpG sites for patient segregation with different clinical outcomes. These findings can be useful for better stratification of patients in order to improve clinical management and treatment outcomes.

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<p>CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway</p>

Cited by 27 publications

References 40 publications

Circular RNA: A promising new star for the diagnosis and treatment of colorectal cancer

Circular RNA: A promising new star for the diagnosis and treatment of colorectal cancer

RAF1 promotes lymphatic metastasis of hypopharyngeal carcinoma via regulating LAGE1: an experimental research

Network analysis after epigenome wide methylation study revealed JUP as a regulator of co-methylated risk-module for T2DM

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