<p>circ_0003418 Inhibits Tumorigenesis And Cisplatin Chemoresistance Through Wnt/β-Catenin Pathway In Hepatocellular Carcinoma</p>

Abstract

BackgroundAccumulating evidences indicate that circRNAs play important roles in the progression and chemoresistance of human cancers. The present study is designated for researching the roles of circ_0003418 in hepatocellular carcinoma (HCC).MethodsWe detected the expression profile of circ_0003418 in human HCC tissues and cell lines by quantitative real-time-PCR assays. CCK-8 assay, transwell migration assay, transwell invasion assay and drug-sensitivity analysis were carried out to estimate the effects of circ_0003418 on HCC cells' proliferation, migration, invasion and resistance to cisplatin, respectively. Mouse xenograft model was conducted to monitor the role of circ_0003418 in cisplatin resistance in vivo. Western blotting was performed to explore the changes of the Wnt/β-catenin pathway after knockdown of circ_0003418. The rescue experiment was carried out to explore circ_0003418-activated biological functions through Wnt/β-catenin pathway.ResultsThe expression level of circ_0003418 was downregulated in HCC tissues and cell lines, and the level correlated with tumor size, TNM stage and HBsAg level in HCC patients. circ_0003418 knockdown promoted HCC cells' proliferation, migration, and invasion. Additionally, suppression of circ_0003418 enhanced cisplatin resistance of HCC cells in vivo and vitro. Knockdown of circ_0003418 activated the Wnt/β-catenin signalling pathway in HCC cells. The effect of circ-0003418 on sensitivity of HCC cells to cisplatin was reversed after inhibition of Wnt/β-catenin pathway.Conclusioncirc-0003418 exerts an antitumorigenic role in HCC and advances the sensitivity of HCC cells to cisplatin by restraining the Wnt/β-catenin pathway. Thus, circ-0003418 may represent a novel biomarker and provide us a new strategy for the treatment of HCC.

<p>Circ_0062020 Knockdown Strengthens the Radiosensitivity of Prostate Cancer Cells</p>

“…Circular RNAs (circRNAs) are a kind of non-coding RNAs, which were documented to be associated with cancers process. [9][10][11][12] The covalent closed loop structure of circRNAs provided more stability due to the lack of poly-A capture, which unleashed the potential of circRNAs used as biomarkers in the diagnosis of cancers. 13 Recently, numerous studies have exposed the momentous role of circRNAs in PCa.…”

<p>Biological Roles and Mechanisms of Circular RNA in Human Cancers</p>

“…11 CircRNAs may also play a key role in the development of drug resistance. Recently, multiple studies have highlighted the key roles of ncRNAs in chemoresistance of cancer, such as HCC, 142,249 lung cancer, 250,251 gastric cancer, 252,253 breast cancer, 254,255 multiple myeloma, 256 acute myeloid leukemia, 234 prostate cancer, 257 bladder cancer, 258 among others. The up-to-date information regarding the role of circRNAs in the resistance of tumors to chemotherapy has been recently summarized with multiple mechanisms, such as modulating various regulatory pathways and processes including the ceRNA regulatory network axis, EMT process, regulation of ABC transporters, apoptosis, autophagy, and CSCs, among others although many physiological processes and biological signaling pathways through which circRNAs are involved in drug resistance still remained unknown.…”

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

“…Hepatocellular carcinoma Circ_0003418 and circRNA_ 101505 are decreased in HCC tissues, while circ-SMARCA5 is reduced in intrahepatic cholangiocarcinoma (ICC) [31,160,161]. Silencing circ_0003418 promotes cisplatin resistance, probably though miR-7-and miR-383mediated effects on the Wnt/β-catenin pathway, but this mechanism still needs further investigation [161]. Cir-cRNA_101505 is especially lower in cisplatin-resistant HCC cells than in sensitive cells [31].…”