<p>Cinnamaldehyde Inhibits the Function of Osteosarcoma by Suppressing the Wnt/β-Catenin and PI3K/Akt Signaling Pathways</p>

Abstract

Background Osteosarcoma (OS) is a primary bone tumor associated with locally aggressive growth and early metastatic potential that typically occurs in children and adolescents. Chinese traditional medicine Cinnamomum cassia Presl has been shown to have significant tumor-killing effect, in which cinnamaldehyde (CA) is the main active ingredient. Purpose To explore the anticancer effect of CA on the osteosarcoma cells and the possible molecular mechanism. Methods Crystal violet assay, MTT assay and colony-forming assay were used to confirm the inhibitory role of CA in the proliferation of 143B and MG63 osteosarcoma cells. Hoechst 33258 staining and flow cytometry were used to observe apoptosis. The migration and invasion role of OS cells were evaluated using transwell assays and wound healing assays. Western blotting was used to analyse the protein expression levels. Nude mice were inoculated with 143B cells to establish an orthotopic OS tumor animal model and to investigate the effects of CA on OS tumors. Results According to crystal violet assay, MTT assay and colony-forming assay, CA significantly inhibited cell proliferation. Hoechst 33258 staining and flow cytometry analysis showed that CA-induced apoptosis in a concentration-dependent manner. In addition, transwell assays and wound healing assays showed that CA inhibited the migration and invasion of osteosarcoma cells. In vivo mouse models, CA inhibited the growth of osteosarcoma. The potential mechanisms could be that CA inhibited the transcriptional activity of Wnt/β-catenin and PI3K/Akt of the osteosarcoma. Conclusion CA may inhibit the proliferation, migration, invasion and promote apoptosis of OS cells by inhibiting Wnt/β-catenin and PI3K/Akt signaling pathways. CA may be a potentially effective anti-tumor drug.

Cinnamaldehyde Suppressed EGF-Induced EMT Process and Inhibits Ovarian Cancer Progression Through PI3K/AKT Pathway

“… Liu et al (2020) used GeneCards and OMIM databases for the enrichment analysis and found that CA is closely related to the AKT/PI3K signaling pathway in breast cancer. Liu and Huang confirmed that CA regulated colorectal cancer and osteosarcoma progressing via the AKT/PI3K signaling pathway ( Li et al, 2016 ; Huang et al, 2020 ). In our present study, Western blotting results showed that CA was able to downregulate the phosphorylation levels of PI3K/AKT pathway proteins in A2780 and SKOV3 cells with or without EGF.…”

Global regulatory factor FsveA up-regulate the production of antitumor substance in endophytic Fusarium solani

“…Study has showed that L-arginine affects the metabolic tness and viability of T cells which were critical for anti-tumor responses (Geiger et al 2016). Cinnamaldehyde inhibits proliferation, migration, invasion and promotes apoptosis of OS cells and is a potentially valid antitumor agent (Huang et al 2020). 4-Allyl-2-methoxyphenol inhibits non-small cell lung cancer by inhibiting NF-B to regulate TRIM59 expression (Cui et al 2019).…”

Anticancer effects of melatonin via regulating lncRNA JPX‐Wnt/β‐catenin signalling pathway in human osteosarcoma cells

“…80 Cinnamaldehyde promotes the cell apoptosis and inhibits the cell proliferation, migration and invasion in OS cells via inhibiting the Wnt/β‐catenin pathway. 81 MiR‐1236‐3p inhibits the progression of OS via targeting Wnt3a 3’ UTR. 82 Similarly, in this study, the Wnt/β‐catenin pathway was also inhibited by melatonin.…”

“…The activation of the Wnt/βcatenin pathway increases the chemoresistance of OS cells 80. Cinnamaldehyde promotes the cell apoptosis and inhibits the cell proliferation, migration and invasion in OS cells via inhibiting the Wnt/β-catenin pathway 81. MiR-1236-3p inhibits the progression of OS via targeting Wnt3a 3' UTR 82.…”