&lt;p&gt;Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats&lt;/p&gt;

Pei, Yilun; Cui, Fuai; Du, Xuejun; Shang, Guowei; Xiao, Wan'an; Yang, Xinlin; Cui, Quanjun

doi:10.2147/ijn.s202466

Cited by 40 publications

(34 citation statements)

References 45 publications

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“…Gram-negative bacterial lipopolysaccharide (LPS) has been widely used in previous studies to induce inflammatory mediators. 29 Accordingly, in the present study, HaCaT cells were treated with LPS to establish the in vitro inflammatory OLP model. Initially, we found that after LPS treatment, the miR-23a-3p levels were downregulated significantly in HaCaT cells, which was consistent with the results observed in buccal mucosal tissues of OLP patients.…”

Section: Discussionmentioning

confidence: 99%

Clinical Values of miR-23a-3p in Oral Lichen Planus and Its Role in Keratinocyte Proliferation and Inflammatory Response

Wang¹,

Hu²,

Li³

2021

JIR

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Purpose Oral lichen planus (OLP) is a chronic inflammatory disease occurring in the oral cavity, and several miRNAs have been identified to be involved in the disease progression and malignant transformation. This study investigated the expression changes of miR-23a-3p in OLP patients, and further explored its functional role in keratinocyte cell proliferation and inflammatory response. Patients and Methods Fifty buccal mucosal tissue samples were collected from OLP patients. HaCaT cells were cultured with lipopolysaccharides (LPS) to mimic the condition of OLP in vitro. RNA extraction and quantitative real-time PCR (qRT-PCR) were used for the measurement of miR-23a-3p levels. The cell viability and inflammation were detected by using cell counting kit-8 (CCK-8) and enzyme-linked immunosorbent assay (ELISA). The target gene of miR-23a-3p was verified by using luciferase reporter assay. Results Compared with the control group, miR-23a-3p was significantly downregulated in the buccal mucosal tissues of OLP patients, and a remarkably decreased level of miR-23a-3p was detected in patients with erosive OLP. ROC curve demonstrated the diagnostic value of miR-23a-3p for OLP with the AUC of 0.908, it can also distinguish erosive OLP from the non-erosive ones. MiR-23a-3p level was negatively associated with RAE (reticular, atrophic, erosive) score in OLP patients (r = −0.790, P < 0.001). The in vitro experiments indicated that overexpression of miR-23a-3p reversed the promotive effect of LPS on HaCaT cell proliferation and reduced the protein levels of TNF-α and IL-6. The cyclin D1 (CCND1) was a direct target gene of miR-23a-3p, it was overexpressed in OLP cell models. Conclusion MiR-23a-3p was at the low expression in OLP patients and showed close association with the disease severity. Overexpression of miR-23a-3p might inhibit keratinocyte proliferation and inflammatory response via targeting CCND1.

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Section: Discussionmentioning

confidence: 99%

Clinical Values of miR-23a-3p in Oral Lichen Planus and Its Role in Keratinocyte Proliferation and Inflammatory Response

Wang¹,

Hu²,

Li³

2021

JIR

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“…The intra-articular injection of fullerenes alone or in combination with HA in surgically induced rabbit models of knee OA showed a dose-dependent fullerene therapeutic effect and synergistic activity with HA [132]. Similarly, fullerol NPs (a polyhydroxylated form of fullerene [133]) showed anti-inflammatory activity in vitro and the ability to prevent synovial inflammation in rat models of OA induced by the intra-articular injection of monoiodoacetate [134]. Carbon nanotubes or graphene-based nanomaterials have also been investigated for OA treatment [135][136][137][138][139]; however, specific studies to target macrophages are lacking.…”

Section: Other Nanomaterial-based Anti-inflammatory Nanoparticlesmentioning

confidence: 97%

Therapeutic Manipulation of Macrophages Using Nanotechnological Approaches for the Treatment of Osteoarthritis

et al. 2020

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Osteoarthritis (OA) is the most common joint pathology causing severe pain and disability. Macrophages play a central role in the pathogenesis of OA. In the joint microenvironment, macrophages with an M1-like pro-inflammatory phenotype induce chronic inflammation and joint destruction, and they have been correlated with the development and progression of the disease, while the M2-like anti-inflammatory macrophages support the recovery of the disease, promoting tissue repair and the resolution of inflammation. Nowadays, the treatment of OA in the clinic relies on systemic and/or intra-articular administration of anti-inflammatory and pain relief drugs, as well as surgical interventions for the severe cases (i.e., meniscectomy). The disadvantages of the pharmacological therapy are related to the chronic nature of the disease, requiring prolonged treatments, and to the particular location of the pathology in joint tissues, which are separated anatomical compartments with difficult access for the drugs. To overcome these challenges, nanotechnological approaches have been investigated to improve the delivery of drugs toward macrophages into the diseased joint. This strategy may offer advantages by reducing off-target toxicities and improving long-term therapeutic efficacy. In this review, we describe the nanomaterial-based approaches designed so far to directly or indirectly manipulate macrophages for the treatment of osteoarthritis.

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“…And it was noticed that innate immune response was in line with the development of OA [ 70 ]. Inhibition of macrophage activation could substantially reduce the inflammatory response and block the OA advancement in rat model [ 71 ]. Given these evidences, we envision that circRNAs are capable of interfering the progression of OA by affecting the innate immune system, which may pave a novel pathway to discover the underlying mechanism of OA.…”

Section: Circrnas In Microenvironment Of Oamentioning

confidence: 99%

Circular RNAs in osteoarthritis: indispensable regulators and novel strategies in clinical implications

Zhang

Chen

et al. 2021

Arthritis Res Ther

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Over the past decades, circular RNAs (circRNAs) have emerged as a hot spot and sparked intensive interest. Initially considered as the transcriptional noises, further studies have indicated that circRNAs are crucial regulators in multiple cellular biological processes, and thus engage in the development and progression of many diseases including osteoarthritis (OA). OA is a prevalent disease that mainly affects those aging, obese and post-traumatic population, posing as a major source of socioeconomic burden. Recently, numerous circRNAs have been found aberrantly expressed in OA tissues compared with counterparts. More importantly, circRNAs have been demonstrated to interplay with components in OA microenvironments, such as chondrocytes, synoviocytes and macrophages, by regulation of their proliferation, apoptosis, autophagy, inflammation, or extracellular matrix reorganization. Herein, in this review, we extensively summarize the roles of circRNAs in OA microenvironment, progression, and putative treatment, as well as envision the future directions for circRNAs research in OA, with the aim to provide a novel insight into this field.

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<p>Antioxidative nanofullerol inhibits macrophage activation and development of osteoarthritis in rats</p>

Cited by 40 publications

References 45 publications

Clinical Values of miR-23a-3p in Oral Lichen Planus and Its Role in Keratinocyte Proliferation and Inflammatory Response

Clinical Values of miR-23a-3p in Oral Lichen Planus and Its Role in Keratinocyte Proliferation and Inflammatory Response

Therapeutic Manipulation of Macrophages Using Nanotechnological Approaches for the Treatment of Osteoarthritis

Circular RNAs in osteoarthritis: indispensable regulators and novel strategies in clinical implications

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