&lt;i&gt;In vivo&lt;/i&gt; evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration

Maglione, Michele; Spano, Serena; Ruaro, Maria Elisabetta; Salvador, Enrico; Zanconati, Fabrizio; Tromba, Giuliana; Turco, Gianluca

doi:10.2334/josnusd.16-0235

Cited by 13 publications

(10 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For chondrogenic or osteogenic regeneration, the spontaneous adipogenic differentiation of ADSCs should be minimized as possible. Adipogenic, chondrogenic, and osteogenic differentiation was observed in passage 3 in many studies (29)(30)(31)(32)(33)(34). The results of the present study indicated that the impact of spontaneous adipogenic differentiation reduced sufficiently after passage 3, and the cells passaged more than three times were probably suitable for chondrogenic and osteogenic regeneration.…”

Section: Discussionsupporting

confidence: 59%

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Yang

Zhang

2018

Mol Med Report

View full text Add to dashboard Cite

Primary adipose-derived stem cells (ADSCs) are a mixture of cell types including preadipocytes having the ability to spontaneously differentiate into adipocytes. The aim of the present study was to compare the spontaneous adipogenic differentiation potential of ADSCs at different passages to determine whether it decreased with continuous cell passages. Mouse ADSCs (mADSCs) were harvested and cells from passages 1 to 5 were used for experiments. The proliferation of mADSCs at different passages was tested using the cell counting kit‑8 assay. Reverse transcription‑quantitative polymerase chain reaction was used to determine relative mRNA expression levels of CCAAT/enhancer binding protein α (Cebpa and C/EBPα) and peroxisome proliferator‑activated receptor γ (Pparg and PPARγ), and western blot analysis was used to investigate C/EBPα and PPARγ protein expression. The cells were cultured using DMEM. The fixed cells were then stained using Oil Red O on days 14 and 28, and the obtained extracted dye was monitored for absorbance. The 510 nm absorbance from passages 1 to 5 was observed to be statistically different. The relative expression levels of Cebpa and Pparg for mADSCs from passage 1 were significantly higher when compared with those for mADSCs from passages 2 to 5 on days 3, 5 and 7. However, no difference was identified in the expression levels of proteins C/EBPα and PPARγ for different passages. Although the mRNA expression levels of Pparg from passages 4 to 5 were significantly higher when compared with those from passage 1, the results of Oil Red O absorbance, mRNA expression levels of Cebpa, and the protein expression levels of C/EBPα and PPARγ exhibited no difference between mADSCs from passages 1 to 5 when cultured with induced adipogenic differentiation medium. Therefore, it was concluded that the spontaneous adipogenic differentiation potential of ADSCs decreased with continuous cell passages.

show abstract

Section: Discussionsupporting

confidence: 59%

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Yang

Zhang

2018

Mol Med Report

View full text Add to dashboard Cite

show abstract

“…Maglione et al. demonstrated the osteogenic potential of ASCs further by seeding them onto a chitosan-glycerol phosphate hybrid gel scaffold, which was maintained by in-situ by cross-linking [382] . The scaffolds were implanted into full-thickness mandible defects, with ex vivo histological and micro CT analysis performed after 8 weeks.…”

Section: Materials Used Within Bone Tissue Engineeringmentioning

confidence: 99%

3D bioactive composite scaffolds for bone tissue engineering

Turnbull

Clarke

Picard

et al. 2018

Bioactive Materials

958

679

View full text Add to dashboard Cite

Bone is the second most commonly transplanted tissue worldwide, with over four million operations using bone grafts or bone substitute materials annually to treat bone defects. However, significant limitations affect current treatment options and clinical demand for bone grafts continues to rise due to conditions such as trauma, cancer, infection and arthritis. Developing bioactive three-dimensional (3D) scaffolds to support bone regeneration has therefore become a key area of focus within bone tissue engineering (BTE). A variety of materials and manufacturing methods including 3D printing have been used to create novel alternatives to traditional bone grafts. However, individual groups of materials including polymers, ceramics and hydrogels have been unable to fully replicate the properties of bone when used alone. Favourable material properties can be combined and bioactivity improved when groups of materials are used together in composite 3D scaffolds. This review will therefore consider the ideal properties of bioactive composite 3D scaffolds and examine recent use of polymers, hydrogels, metals, ceramics and bio-glasses in BTE. Scaffold fabrication methodology, mechanical performance, biocompatibility, bioactivity, and potential clinical translations will be discussed.

show abstract

“…Hydrogels based on carboxymethyl chitosan (CMC) and oxidized chondroitin sulfate (OCS) were prepared and loaded with bovine serum albumin (BSA) for in vitro degradation and BSA release of the CMs/gel scaffolds evaluation [26]. In vivo evaluation of chitosan-glycerol incorporating stem cells were evaluated for mandibular bone regeneration, exhibiting an exciting osteogenic potential [27]. Relevant literature for hydrogel and nanogels for biomedical applications has been published recently [28].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films

et al. 2020

View full text Add to dashboard Cite

The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world. Tissue engineering offers multiple options from biocompatible materials with easy resorption. Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications. In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions. An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films. Infrared spectroscopy (FTIR) analyses demonstrated the presence of RGEO. In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation. Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v). The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials. On the other hand, the thermal analysis showed that the RGEO incorporation almost did not affect thermal degradation. However, mechanical properties demonstrated an understandable loss of tensile strength and Young’s modulus for F3 and F4. However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films. The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed. All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.

show abstract

<i>In vivo</i> evaluation of chitosan-glycerol gel scaffolds seeded with stem cells for full-thickness mandibular bone regeneration

Cited by 13 publications

References 41 publications

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

Spontaneous adipogenic differentiation potential of adipose‑derived stem cells decreased with increasing cell passages

3D bioactive composite scaffolds for bone tissue engineering

Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films

Contact Info

Product

Resources

About