<i>In Vivo</i> Activity of (8-Hydroxymethylen)-Trieicosanyl Acetate against <i>Trypanosoma cruzi</i> during Acute Phase of the Infection

Abstract

The antiprotozoal activity in vivo against Trypanosoma cruzi of (8-hydroxymethylen)-trieicosanyl acetate was evaluated in BALB/c mice during the acute phase of Chagas' disease (15 days after infection). Animals were treated during 15 days at doses of 16.8 and 33.6 µg/g, reduced parasitemia of 77.6 and 64.1% was observed respectively, in comparison with positive control mice (allopurinol 8.5 µg/g) which reduced only 29.7%. Also, amastigote nests in cardiac tissue were significant reduced in treated mice groups. The regression of effect induced after the suppression of the treatment with the compound was evaluated; animals were infected and simultaneously began the treatment with the compound during 20 days (16.8 and 33.6 µg/g). Mice were monitored after the end of the treatment for one more week. A good antitrypanosomal response was observed (66.1 and 68.9% less than untreated mice) during treatment, but 8 days after suspension of treatment, parasitemia level increased, reducing only 58.6 and 56.29 % respectively in treated animals compared with no treated. RésuméL'activité in vivo contre antiprotozoaires Trypanosoma cruzi de (8-hydroxymethylen)-acétate de trieicosanyl a été évaluée chez des souris BALB / c pendant la phase aiguë de la maladie de Chagas (15 jours après l'infection). Les animaux ont été traités pendant 15 jours à des doses de 16,8 et 33,6 mg / g, la parasitémie réduite de 77,6 et 64,1% a été observée, respectivement, en comparaison avec les souris de contrôle positif (allopurinol 8,5 mg / g), qui réduit que de 29,7%. En outre, les nids amastigote dans le tissu cardiaque ont été réduits dans les groupes importants chez les souris traitées. La régression de l'effet induit après la suppression du traitement avec le composé a été évalué, les animaux ont été infectés et, simultanément, a commencé le traitement avec le composé pendant 20 jours (16,8 et 33,6 mg / g). Les souris ont été suivis après la fin du traitement pour une semaine de plus. Une bonne réponse a été observée antitrypanosomal (66,1 et 68,9% de moins que les souris non traitées) en cours de traitement, mais 8 jours après la suspension du traitement, le niveau de la parasitémie a augmenté, réduisant seulement 58,6 et 56,29% respectivement chez les animaux traités par rapport à aucun traitement.

Review on Experimental Treatment Strategies Against Trypanosoma cruzi

“… [ 131 ] Senna villosa (8-hydroxymethylen)-trieicosanyl acetate Isolated compound showed in vitro and in vivo activity against T. cruzi . [ 122 , 125 ] Zanthoxylum chiloperone Canthin-6-one alkaloids and leaves extract Canthin-6-one alkaloids exhibited trypanocidal activity in vitro and in mouse model of acute or chronic infection. Leaves extract reduced parasitaemia in vivo.…”

“…Natural products isolated from different botanical sources exhibited activity against T. cruzi (see Table 2). [120][121][122][123][124][125][126][127][128][129][130][131][132] Several approaches have been developed focusing on identification and isolation of plantbased products with anti-T. cruzi activity 133,134 (Table 2). In this sense, extracts from different plants as Salvia, Valeriana, Hypericum, Silybum, Arnica, and Curcuma showed activity against T. cruzi.…”

“…Isolated compound showed in vitro and in vivo activity against T. cruzi. [122,125] Zanthoxylum chiloperone…”

Bioassay-Guided Chemical Study of the Anti-Inflammatory Effect of Senna villosa (Miller) H.S. Irwin & Barneby (Leguminosae) in TPA-Induced Ear Edema

“…An aqueous extract is also used for the treatment of irregular menstruation and inflammatory problems [32]. It has been reported to have trypanocidal and antiprotozoal activities [33,34,35,36,37,38]. However, the anti-inflammatory activity of this plant and its active components has not yet been systematically studied.…”