<i>In Vitro</i> Nitric Oxide Scavenging Activity of Ethanol Leaf Extracts of Four Bangladeshi Medicinal Plants

Abstract

The ethanol leaf extracts of four medicinal plants named Hibiscus mutabilis, Leucas aspera, Ixora coccinea and Polyalthia longifolia were examined for their possible regulatory effect on nitric oxide (NO) levels using sodium nitroprusside as a NO donor in vitro. Most of the extracts tested demonstrated direct scavenging of NO and exhibited significant activity and the potency of scavenging activity was in the following order: Leucas aspera > Ixora coccinea > Hibiscus mutabilis> Polyalthia longifolia. All the evaluated extracts exhibited a dose-dependent NO scavenging activity. The ethanolic leaf extract of Leucas aspera showed the greatest NO scavenging effect of 80.50% at 320 µg/ml with IC50 value of 94.15 µg/ml as compared to the positive control ascorbic acid where 74.56 % scavenging was observed at similar concentration with IC50 value of 62.48 µg/mL. The maximum NO scavenging of Ixora coccinea, Hibiscus mutabilis and Polyalthia longifolia were 79.65 %, 78.60% and 70.67 % with IC50 values of 43.72 µg/ml, 147.64 µg/ml and 167.08 µg/ml respectively. The present results suggest that these plants might be potent and novel therapeutic agents for scavenging of NO and the regulation of pathological conditions caused by excessive generation of NO and its oxidation product.

Evaluation of phytochemical composition and antioxidant capacity of a decoction containing Adenanthera pavonina L. and Thespesia populnea L.

“…These compounds are responsible for altering the structural and functional behavior of many cellular components. [ 26 ] Nitric oxide produced by SNP over 2-hour incubation at 25°C has been scavenged by the decoction. The decoction used in the present study contains strong nitric oxide scavenging activity with an EC 50 of 14.02 ± 0.66 μg/ml as compared with Pyrus boissieriana , Cnidium officinale , and Ligusticum chuanxiong , which showed an EC50 of 650, 57.25, and 76.50 μg/ml, respectively.…”

Computational Analysis of Pyocyanin (Phz Gene) From Pseudomonas

“…This activity may allow P. aeruginosa an advantage over competing bacteria occupying the same niche. Furthermore, pyocyanin has a variety of pharmacological effects on eukaryotic and prokaryotic cells [5]. [6,7] indicated that pyocyanin has antimicrobial activity against bacteria, fungi, and protozoa, but it is little therapeutic value because it is quite toxic to eucaryotic cells, since pyocyanin inhibited mammalian cell respiration disrupt the beating of human cilia, and inhibit both epidermal cell growth and lymphocyte proliferation.…”

Annonaceae: Breaking the Wall of Inflammation

“…Results from present study suggested that 16-hydroxycleroda-3,13-dien-15,16-olide (23) and 16-oxocleroda-3,13-dien-15-oic acid (24) (Figure 1 ) has significantly ( P < 0.05) reduced NO production at 10 μg/mL, with 81.1 and 86.3%, inhibition, respectively. Similarly, Saha et al ( 2008 ) conducted a study on Bangladeshi medicinal Plants ( Hibiscus mutabilis, Leucas aspera, Ixora coccinea , and P. longifolia) . Ethanolic extract of all the tested species showed dose dependent NO direct scavenging activity in following manner L. aspera > I. coccinea > H. mutabilis > P. longifolia .…”