&lt;i&gt;In vitro&lt;/i&gt; cytotoxic evaluation of novel fast-setting calcium silicate cement compositions and dental materials using colorimetric methyl-thiazolyl-tetrazolium assay

Ranjkesh, Bahram; Isidor, Flemming; Kraft, David C.; Løvschall, Henrik

doi:10.2334/josnusd.16-0751

Cited by 20 publications

(22 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in vitro cytotoxicity level of the novel calcium silicate cement has been shown to be similar to other calcium silicate-based cements (MTA and Biodentine) and superior to resin-modified glass ionomer cements, zinc phosphate cement, calcium hydroxide-based material, and zinc oxide eugenol-based cement [31]. Acceptable biocompatibility, periapical tissue tolerance, and clinical performance of the novel calcium silicate cement after three years have recently been reported [32].…”

Section: Introductionmentioning

confidence: 70%

Direct pulp capping of primary molars using a novel fast-setting calcium silicate cement: a randomized clinical trial with 12-month follow-up

Vafaei

Azima

Erfanparast

et al. 2019

Biomaterial Investigations in Dentistry

Self Cite

View full text Add to dashboard Cite

Objective: Novel fast-setting calcium silicate cement with fluoride (Protooth) has been developed for potential applications in tooth crowns. The aim of this study was to evaluate the success rate of direct pulp capping in primary molars using two-layer mineral trioxide aggregate (MTA) and overlying glass ionomer cement versus one-layer novel calcium silicate cement with 4 to 10 minutes setting time. Materials and methods: Ninety bilaterally symmetrical primary molars in the same jaw in 45 patients aged 5 to 7 years were included. Exposed pulps following caries removal were randomly capped with one-layer novel calcium silicate cement or two-layer MTA and glass ionomer cement. All cavities were filled with amalgam. Clinical and radiographic evaluations were performed after six and twelve months. 41 patients were available for the evaluations at the end of the 12-month follow-up. Results: The overall success rate of direct pulp capping, in a split-mouth design, using MTA covered with glass ionomer cement or one-layer novel calcium silicate cement after 12 months were 90% (37 out of 41 cases) and 85% (35 out of 41 cases), respectively, without statistically significant differences after 6 and 12 months. Conclusion: Within the limitations of this study, clinical and radiographic evaluations suggested one-layer novel calcium silicate cement would be successfully used in direct pulp capping of primary molars as a practical alternative to two-layer MTA and overlying glass ionomer cement.

show abstract

Section: Introductionmentioning

confidence: 70%

Direct pulp capping of primary molars using a novel fast-setting calcium silicate cement: a randomized clinical trial with 12-month follow-up

Vafaei

Azima

Erfanparast

et al. 2019

Biomaterial Investigations in Dentistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…To date, the fast setting time, improved mechanical properties , apatite‐forming or mineralizing ability , biocompatibility , acceptable clinical results , interfacial gap closure capability , and superior long‐term bonding to dentine, as observed in this study, support further testing on Protooth for potential applications in the tooth crown. Accordingly, Protooth may be a candidate for pulp protection, treatment of endodontic complications, caries sealing, and post or crown cementation.…”

Section: Discussionmentioning

confidence: 99%

Bond strength between dentine and a novel fast‐setting calcium silicate cement with fluoride

Ranjkesh

Kopperud

et al. 2019

European J Oral Sciences

Self Cite

View full text Add to dashboard Cite

Bond strength between dentine and a novel fast-setting calcium silicate cement with fluoride Ranjkesh B, Kopperud HM, Kopperud SE, Løvschall H. Bond strength between dentine and a novel fast-setting calcium silicate cement with fluoride. Eur J Oral Sci 2019; 127: 564-569. © 2019 Eur J Oral SciThe aim of this study was to evaluate the dentine bond strength of a novel fast-setting calcium silicate cement (Protooth) versus a calcium hydroxide-based cement (Dycal), a calcium silicate cement (ProRoot MTA), and a glass ionomer cement (Ketac-Molar). Mid-root dentine slices of 1 mm thickness were obtained from human maxillary incisors. After enlarging the lumen of the canal to 1.3 mm, the cavities were randomly filled with test materials. Samples were immersed in physiological-like solution. The push-out bond strength was tested on days 1, 28, and 180 (n = 12). Failure types of bonding were determined using a stereomicroscope. We analysed the data using linear regression. Dycal and day 1 were considered as reference for cement type and assessment time, respectively. Protooth, Ketac-Molar, and ProRoot MTA demonstrated higher push-out bond strength than Dycal. The pushout bond strength in the Protooth group increased on day 28 and 180. The bond strength of Ketac-Molar was significantly reduced on day 28. Dycal showed a significant decrease in bond strength on day 180 compared with that on day 1 and 28. Mixed failure was the dominant failure type. Protooth bonding to dentine was increased with time, in contrast to that of ProRoot MTA, Dycal, and Ketac-Molar, as a function of time.

show abstract

“…2018, Ranjkesh et al . 2018, Pravinchandra et al . 2018), extremely variable results have been reported considering the culture models used for investigation of its cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Anti‐inflammatory and immunomodulatory effects of Biodentine on human periapical lesion cells in culture

Eraković

Duka

Bekić³

et al. 2020

Int Endodontic J

View full text Add to dashboard Cite

Aim To evaluate the effect of Biodentine eluate on cytotoxicity and production of pro‐ and anti‐inflammatory cytokines and osteodestructive/osteoprotective cytokines in cultures of human periapical lesion cells. Methodology Conditioned Biodentine Medium (CBM) was prepared according to ISO 10993‐12, by incubating Biodentine in RPMI medium (0.2 g mL−1) for 3 days at 37 °C. CBM contained both released microparticles and leachable soluble components. Inflammatory cells were isolated from 22 human periapical lesions after apicectomy or tooth extraction, by collagenase/DNase digestion, and cultured in several dilutions of CBM. The composition of periapical lesion cells was determined by morphological criteria, cytotoxicity was quantified by MTT and flow cytometric apoptosis/necrosis assays, whereas the levels of produced cytokines in cell culture supernatants were measured by flow cytometry and ELISA. Student t‐test and Friedman test with Dunn's post‐test were used for comparison of parametric and nonparametric variables, respectively. Results Undiluted (100%), 75% and 50% CBM were cytotoxic for periapical lesion cells due to induction of both necrosis (100% CBM) and apoptosis (75% and 50% CBM). Noncytotoxic concentrations of CBM (25%) inhibited the production of pro‐inflammatory cytokines: TNF‐α, (P < 0.005); IL‐1β (P < 0.01); IL‐6 (P < 0.005) and chemokines IL‐8: (P < 0.005); MCP‐1 (P < 0.005), stimulated the production of anti‐inflammatory cytokine (IL‐10; P < 0.005), Th2 cytokines: IL‐4, IL‐5 and IL‐33 (all P < 0.01), and IL‐17A (P < 0.01). The concentration of CBM (12.5%) inhibited the production of IL‐6 (P < 0.05), IL‐8 (P < 0.01) and MCP‐1 (P < 0.005) and augmented the production of IL‐10 (P < 0.05). No significant effects on Th1‐related cytokines (IFN‐γ and IL‐12) and IL‐23 were detected with 25% and 12.5% CBM concentrations. Both CBM concentrations inhibited the production of osteolytic receptor activator of nuclear factor kappa‐Β ligand (RANKL), dose dependently (P < 0.005 and P < 0.01, respectively). Higher CBM concentrations decreased RANKL/osteoprotegerin (OPG) ratio (P < 0.05), without significant influence on the levels of osteoprotective OPG. Conclusion Biodentine possesses immunomodulatory properties by suppressing pro‐inflammatory and augmenting anti‐inflammatory cytokines. Together with the reduction of osteodestructive mediators, this novel root‐end filling cement could be beneficial for healing and bone reparation after the surgical treatment of periapical lesions.

show abstract

<i>In vitro</i> cytotoxic evaluation of novel fast-setting calcium silicate cement compositions and dental materials using colorimetric methyl-thiazolyl-tetrazolium assay

Cited by 20 publications

References 40 publications

Direct pulp capping of primary molars using a novel fast-setting calcium silicate cement: a randomized clinical trial with 12-month follow-up

Direct pulp capping of primary molars using a novel fast-setting calcium silicate cement: a randomized clinical trial with 12-month follow-up

Bond strength between dentine and a novel fast‐setting calcium silicate cement with fluoride

Anti‐inflammatory and immunomodulatory effects of Biodentine on human periapical lesion cells in culture

Contact Info

Product

Resources

About