&lt;i&gt;Escherichia coli&lt;/i&gt; Isolates from Calf Diarrhea in Korea and Their Virulent Genetic Characteristics

Hur, Jin; Jeon, Byung Woo; Kim, Yeong Ju; Oh, In Gyeong; Lee, John Hwa

doi:10.1292/jvms.12-0378

Cited by 9 publications

(8 citation statements)

References 15 publications

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“…PCR detection of 16 E. coli isolates showed that the detection rate of eaeA genes was 12.50% ( Fig. 1, Table 3) which is correlated with Hur et al, 2013 in Korea who reported 13-17% (from diarrheic calves) of the same gene. From other studies the reported range of eaeA gene was 1.2% to 9.8% (Yuluo et al, 2010;Nguen et al, 2010;Salehi et al, 2011).…”

Section: Detection Of Pcr Productssupporting

confidence: 61%

Investigation of Pathogenic <i>Escherichia Coli</i> from Diarrheic Calves in Selective Area of Bangladesh

Islam¹,

Rahman²,

Nahar³

et al. 2015

Bangl. J. Vet. Med.

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Molecular technique was used to investigate the prevalence of virulent diarrheic genes in pathogenic Escherichia coli and their antibiotic sensitivity patterns. A hundred samples from 100 different diarrheic calves from mid-north-western part of Bangladesh were screened for the presence of virulence factors associated with diarrhea. Following isolation and identification on the basis of cultural, morphological and biochemical properties, the presence of the virulence genes such as eaeA, bfpA, elt, est, stx1 and stx2 were examined using PCR. Antimicrobial susceptibility of 57 E. coli was determined by agar disk diffusion method for 8 antimicrobial agents. Out of 100 samples 57 (57%) were found to be positive for E. coli and their distribution rates according to their age, breed and sex were 66.7% ( 6 days old ), 85.7% (Sahiwal breed) and in 64.2 % (female calves) respectively. Among 57 E. coli isolates, only 16 isolates were analyzed for the detection of the said genes. Among them, only eaeA gene was detected in 2 E. coli isolates (12.5 %). Antibiotic resistance patterns revealed that Oxacillin, Rifampicin and Penicillin were 100% resistant followed by Erythromycin which was more than 80% resistant. In case of Amoxicillin and Tetracycline, about 59.65% and 61.40% were found to be resistant respectively whereas all 57 E. coli isolates showed moderately susceptible (30%) to Cefuroxime, a second generation Cephalosporin. Therefore, none of the eight antimicrobials studied can not be recommended as single best therapeutic agent for the treatment of neonatal calf diarrhea. In addition, this study indicated that diarrhea in calves in these locations can be ascribed to mainly Enteropathogenic E. coli (EPEC) which was atypical (only contained the eaeA genes but not bfpA). However, further studies are necessary to characterize the isolated eaeA gene positive E. coli by serotyping, tissue culture assay and other molecular techniques to find out the potentiality of those virulent genes contributing pathogenicity of E. coli causing diarrhea in calves.

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Section: Detection Of Pcr Productssupporting

confidence: 61%

Investigation of Pathogenic <i>Escherichia Coli</i> from Diarrheic Calves in Selective Area of Bangladesh

Islam¹,

Rahman²,

Nahar³

et al. 2015

Bangl. J. Vet. Med.

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“…The EPEC pathotype represented 8% of E . coli isolates, slightly lower than 12% [ 75 ] and 13% [ 76 ] detection rates in Bangladesh and Korea, respectively. A decreased frequency of EPEC strain was also reported by [ 77 – 79 ] in Turkey, Vietnam, and Iran respectively.…”

Section: Discussionmentioning

confidence: 81%

Molecular identification of diarrheagenic Escherichia coli pathotypes and their antibiotic resistance patterns among diarrheic children and in contact calves in Bahir Dar city, Northwest Ethiopia

et al. 2022

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Diarrheagenic Escherichia coli strains are an essential cause of diarrheal infection in younger children and animals. The study was focused on understanding the associated characteristics of various DEC strains among children and calves, establishing the possible zoonotic transmission, and determining their antibiotic resistance patterns. Samples from 144 acute diarrheic children and 50 diarrheic calves were collected and processed using traditional culture methods. The molecular identification of pathotypes was completed using primer-specific polymerase chain reaction (PCR) targeting ten virulence genes (stx1, stx2, eae, aatA, lt, st, ial, hlyA bfpA, and daaE) related to six DEC pathotypes (EPEC, ETEC, EHEC, EAEC EIEC, and DAEC). The antimicrobial susceptibility testing was carried out using the Kirby-Bauer disk diffusion method. Colonies from 74 study subjects (54 diarrheic children and 20 diarrheic calves) were positive for E. coli isolates. Subsequent PCR detection discovered that 77% of children and 85% of calves’ isolates were positive for one or more virulence genes typical of particular strains. Among those ETEC [(18%), (26%)] is being the maximum predominant, and [(15%), (15%)] were positive for STEC, [(13%), (8%)] for atypical EPEC, [(6%), (7%)] for EHEC, [(6%), (5%)] for EAEC, and [(6%), (4%)] for EIEC strains in children’s and calves, respectively. Of the identified E. coli isolates, about 29% were found to be hybrid isolates. ETEC (66.7%) and STEC (58.9%) strains showed a better detection rate in contact children with diarrheic calves than children with no contacts. Most antibiotic resistances were obtained towards amoxicillin (64.9%), gentamycin (56.8%), and ampicillin (54.1%). Up to sixty-five percent of isolates were resistant to a minimum of three categories of antibiotics. This is the primary report on the wide occurrence of the six-diarrheagenic Escherichia coli strains, and ETEC was found to be the predominant pathotype among children and contact calves in Ethiopia.

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“…Escherichia coli has been reported as among the most common pathogens associated with diarrhea in calves, leading to high morbidity and mortality worldwide. However, remarkably few studies have been performed to evaluate the relationship between E. coli and calf diarrhea in the ROK [ 18 – 20 ]. In the present study, we examined the prevalence of E. coli according to age and explored the possibility of associations between E. coli and diarrhea in pre-weaned calves.…”

Section: Discussionmentioning

confidence: 99%

Characterization of virulence genes in Escherichia coli strains isolated from pre-weaned calves in the Republic of Korea

et al. 2020

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Background Escherichia coli is an important cause of diarrhea in calves and its diarrheagenic properties are related to presence of certain virulence genes. In this study, the prevalence of virulence genes F5 , F17 , F41 , sta , stx1 , stx2 , eae , and saa in E. coli isolated from pre-weaned calves presenting with (n= 329) or without diarrhea (n= 360) was explored using multiplex polymerase chain reaction. We also evaluated the association between detection of E. coli and the presence of diarrhea. Results Escherichia coli was detected in 56.3% (388/689) of the fecal samples and showed the highest prevalence (66.5%) in 21–40-day-old calves and the lowest (46.3%) among those that were 1–20 days old. The prevalence of the enterotoxigenic E. coli (ETEC) and Shiga toxin-producing E. coli (STEC) pathotypes was detected in 73.9% and 15.9%, respectively. The results showed no association between diarrhea and the presence of E. coli in general, ETEC or STEC. The F17 gene was the most frequently detected virulence factor in E. coli of calves of all ages regardless of diarrhea. Interestingly, the results show that the calves aged 41–60 days with F17 -positive E. coli are at a higher risk for production of Shiga toxin ( Stx1 ; 95% confidence intervals: 1.86–31.95; P = 0.005) compared to calves aged 1–20 days; no association between this finding and diarrhea was observed among the calves of this age group. Moreover, the virulence genes associated with the ETEC and STEC strains were not significantly associated with pathogenicity in this study cohort. Conclusions These results suggest that while the incidence of E. coli is age-related, there was no relationship linking E. coli virulence genes to calf age and diarrhea. Furthermore, the present study demonstrated that detection of E. coli strains either with or without virulence factors was not associated with diarrhea in pre-weaned calves.

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<i>Escherichia coli</i> Isolates from Calf Diarrhea in Korea and Their Virulent Genetic Characteristics

Cited by 9 publications

References 15 publications

Investigation of Pathogenic <i>Escherichia Coli</i> from Diarrheic Calves in Selective Area of Bangladesh

Investigation of Pathogenic <i>Escherichia Coli</i> from Diarrheic Calves in Selective Area of Bangladesh

Molecular identification of diarrheagenic Escherichia coli pathotypes and their antibiotic resistance patterns among diarrheic children and in contact calves in Bahir Dar city, Northwest Ethiopia

Characterization of virulence genes in Escherichia coli strains isolated from pre-weaned calves in the Republic of Korea

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