&lt;b&gt;&lt;i&gt;MMP13&lt;/i&gt;&lt;/b&gt; Contributes to Dental Caries Associated with Developmental Defects of Enamel

Vasconcelos, Katia Regina Felizardo; Arid, Juliana; Evangelista, S.; Oliveira, Sara; Dutra, André Luiz Tannus; Silva, Léa Assed Bezerra; Silva, Léa Assed Bezerra da; Vieira, Alexandre R.; Filho, Paulo Nelson; Küchler, Érika Calvano

doi:10.1159/000496372

Cited by 15 publications

(19 citation statements)

References 29 publications

(39 reference statements)

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“…This resulted in 21 publications including a total of 24 studies that met the eligibility criteria and were included in our analyses 5, 10-12, 21-38 . In summary, the meta-analyses of rs12640848 in ENAM included seven studies with a total of 1,256 subjects in the case group and 710 subjects in the control group 12, 25, 27, 33, 34, 37 ; the meta-analyses of rs1784418 in MMP20 included five studies with a total of 699 subjects in the case group and 817 subjects in the control group 10, 24, 26, 30, 36 ; the meta-analyses of rs17878486 in AMELX included four studies with a total of 249 subjects in the case group and 193 subjects in the control group 10, 12, 22, 25 ; and the meta-analyses of rs3796704 in ENAM included four studies with a total of 574 subjects in the case group and 533 subjects in the control group 12, 25, 32, 34 . Data for other genetic variants came from fewer studies.…”

Section: Resultsmentioning

confidence: 99%

Association of genetic variants in enamel-formation genes with dental caries: A meta- and gene-cluster analysis

Liu

Yang

et al. 2020

Preprint

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Previous studies have reported the association between multiple genetic variants in enamel formation-related genes and the risk of dental caries with inconsistent results. We performed a systematic literature search of the PubMed, Cochrane Library, HuGE and Google Scholar databases for studies published before March 21, 2020 and conducted meta-, gene-based and gene-cluster analysis on the association between genetic variants in enamel- formation-related genes and the risk of dental caries. Our systematic literature search identified 21 relevant publications including a total of 24 studies for analysis. The genetic variant rs17878486 in AMELX was significantly associated with dental caries risk (OR=1.40, 95% CI: 1.02-1.93, P=0.037). We found no significant association between the risk of dental caries with rs12640848 in ENAM (OR=1.15, 95% CI: 0.88-1.52, P=0.310), rs1784418 in MMP20 (OR=1.07, 95% CI: 0.76-1.49, P=0.702) and rs3796704 in ENAM (OR=1.06, 95% CI: 0.96-1.17, P=0.228). Gene-based analysis indicated that multiple genetic variants in AMELX showed joint association with the risk of dental caries (6 variants; P<10-5), so did genetic variants in MMP13 (3 variants; P=0.004), MMP2 (3 variants; P<10-5), MMP20 (2 variants; P<10-5) and MMP3 (2 variants; P<10-5). The gene-cluster analysis indicated a significant association between the genetic variants in this enamel-formation gene cluster and the risk of dental caries (P<10-5). The present meta-analysis revealed that genetic variant rs17878486 in AMELX were associated with dental caries, and multiple genetic variants in enamel-formation-related genes jointly contribute to the risk of dental caries, supporting the role of genetic variants in the enamel-formation genes in the etiology of dental caries.

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Section: Resultsmentioning

confidence: 99%

Association of genetic variants in enamel-formation genes with dental caries: A meta- and gene-cluster analysis

Liu

Yang

et al. 2020

Preprint

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“…The second subset included pre-adolescents aged 9 to 12 years recruited from four public schools in Manaus, state capital Amazonas, Brazil. 26,27 All children who met the inclusion criteria and whose the parents/legal guardians agreed to participate, were included. During the clinical examination, those preschool children with dental treatment needs were referred to dental treatment.…”

Section: Methodsmentioning

confidence: 99%

Association of taste receptor gene polymorphisms with dental caries

et al. 2020

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“…The matrix metalloproteinases (MMPs) constitute a family of secreted and membrane-associated zinc-dependent endopeptidases that are the major regulators of extracellular matrix turnover and are capable of selectively degrading a wide spectrum of both extracellular matrix and non-matrix proteins [20,21]. Matrix metalloproteinase 2, also known as gelatinase A, is a membrane-bound protein with collagen-degrading ability [20,22] and has important roles in tooth formation and mineralization [23][24][25][26][27].…”

Section: Plos Onementioning

confidence: 99%

“…Matrix metalloproteinase 2, also known as gelatinase A, is a membrane-bound protein with collagen-degrading ability [20,22] and has important roles in tooth formation and mineralization [23][24][25][26][27]. Genetic polymorphisms in enamel formation genes could contribute to structural alterations that could lead to enamel porosity, presence of enamel crystal inhibitory proteins and decreased mineral content [21,28,29]. Some studies have demonstrated the expression of MMP2 during enamel formation [23][24][25].…”

Section: Plos Onementioning

confidence: 99%

“…Previous studies have demonstrated that genes involved in enamel development are associated with DDE [21,48,49]. Also, studies have shown the association of MMP2 with formation and mineralization of dental tissues [23][24][25], and dental conditions such as periapical lesions [50], non-carious loss of extensive composite resin restorations [22], and talon cusp [51].…”

Section: Plos Onementioning

confidence: 99%

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Maxillary incisor enamel defects in individuals born with cleft lip/palate

et al. 2020

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Cleft lip with or without cleft palate (CLP) is considered the most frequent congenital malformations of the head and neck, with cleft individuals exhibiting more chances of presenting abnormalities such as developmental defects of enamel (DDE). Matrix metallopeptidase 2 (MMP2) is a membrane-bound protein with collagen-degrading ability and has important roles in tooth formation and mineralization. The aim of this study was to evaluate the frequency, location, severity and extent of DDE found in the maxillary incisors for groups of individuals born with CLP, as well as understanding their relationship with the cleft side. Besides, this study addresses the hypothesis that DDE can be influenced by variation in the MMP2 genes (rs9923304). Individual samples, clinical history, intraoral photographs and panoramic radiographs were obtained from 233 patients under treatment at the Cleft Lip and Palate Service of the University Hospital Lauro Wanderley at the Federal University of Paraíba. Digital images were examined by the same evaluator using the Classification of Defects According to the Modified DDE Index, and then loaded into the Image Tool software, where two measurements were made: total area of the buccal surface (SA) and the area of the DDE (DA), obtaining the percentage of the surface area affected (%SAD) (ICC = 0.99). Genomic DNA was extracted from saliva samples from 124 participants. Genotyping was carried out using TaqMan chemistry for one marker in MMP2 (rs9923304). Statistical analyses were performed by The Jamovi Project software. The Shapiro-Wilk test was applied, followed by the Student’s t-test and the Mann-Whitney test. Chi-square and Fisher’s exact tests, and odds ratio (OR) with 95% confidence interval (CI) calculations were used to determine Hardy-Weinberg equilibrium and statistically significant differences with an alpha of 0.05. No significant differences in the prevalence and extent of enamel defects were found between male and female individuals born with CLP (p = 0.058256). The frequency of individuals presenting teeth with DDE, in relation to the cleft and non-cleft side, was statistically different (p <0.001; OR = 7.15, CI: 4.674> 7.151> 10.942). However, the averages of %SAD were similar (p = 0.18). The highest means of the %SAD were found in individuals with bilateral cleft lip with or without cleft palate (BCLP) when compared to individuals with unilateral cleft lip with or without cleft palate (UCLP), for the teeth inside (IA) and outside the cleft area (OA) (p <0.001). Regardless of the cleft side, individuals with BCLP were 7.85 times more likely to have more than one third of the tooth surface affected, showing more frequently defects in the three thirds (OA: p <0.001) (IA: p = 0.03), as well as a higher frequency of more than one type of defect (OA: p = 0.000358) (IA: p = 0.008016), whereas in UCLP, defects were isolated and restricted to only one third, more frequently, the incisal third (OA: p = 0.009) (IA: p = 0.001), with greater frequency of milder defects, such as demarcated (p = 0.02) and diffuse (p = 0.008) opacities. A higher frequency of the T allele, less common, was observed in the group of CLP individuals who had all the affected teeth or at least two teeth with %SAD greater than 20% (p = 0.019843). Our results suggest that MMP2 may have a role in the cases that presented DDE and genotyping rs9923304 could serve as the basis for a genomic approach to define risks for individuals born with CLP. Frequency and severity of DDE is strongly related to the CLP phenotype, since the highest values were found for BCLP. However, the extent of the DDE is independent of its relationship with the side of the cleft.

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<b><i>MMP13</i></b> Contributes to Dental Caries Associated with Developmental Defects of Enamel

Cited by 15 publications

References 29 publications

Association of genetic variants in enamel-formation genes with dental caries: A meta- and gene-cluster analysis

Association of genetic variants in enamel-formation genes with dental caries: A meta- and gene-cluster analysis

Association of taste receptor gene polymorphisms with dental caries

Maxillary incisor enamel defects in individuals born with cleft lip/palate

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