&lt;b&gt;Enhancing osteoblast-affinity of titanium scaffolds for bone engineering by use of ultraviolet light tr&lt;/b&gt;&lt;b&gt;eatment &lt;/b&gt;

Ishijima, Manabu; Soltanzadeh, Pooya; Hirota, Makoto; Tsukimura, Naoki; Shigami, Tomohiko; Ogawa, Takahiro

doi:10.2220/biomedres.36.55

Cited by 13 publications

(15 citation statements)

References 24 publications

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“…In addition, researchers have shown that the titanium implant surface is easily contaminated by hydrocarbons when stored under common conditions protected from light, which can lead to biological aging. 8 − 10 Our test results also confirmed that the SLA group carried the most negative charges. We used calcium ions carrying a bivalent positive charge as bridging ions.…”

Section: Discussionsupporting

confidence: 77%

Biological Effects of Titanium Surface Charge with a Focus on Protein Adsorption

Ding

et al. 2020

ACS Omega

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The effect of changes in surface charge on the biological properties of implants is not clear. The objective of this study was to evaluate the biological properties of the surface of titanium sheets with different charges due to different treatment methods. Titanium sheets were sandblasted with large grit and underwent acid etching before being subsequently divided into the following groups: SLA, no further treatment; SLA-Ca 2+ , immersed in 1% CaCl 2 solution; SLA-NaCl, immersed in saline; and SLA-Ca 2+ -NaCl, immersed in 1% CaCl 2 solution followed by saline. Surface characteristics were evaluated using field-emission scanning electron microscopy with energy-dispersive spectrometry, surface profilometry, and contact angle assays. Additionally, we used a ζ-potential analyzer to directly measure the electrostatic charge on the different group surfaces. The effect of changes in the Ti surface on biological processes after different treatments was determined by analyzing fibronectin adsorption, osteoblast-like MG63 cell adhesion and proliferation, and the expression of osteogenesis-related genes. Compared to the SLA surface, the other three groups contained corresponding trace elements because they were soaked in different liquids; the contact angles of the three groups were not significantly different, but they were significantly smaller than that of the SLA group; and there was no change in the surface topography or roughness. Furthermore, the SLA-Ca 2+ group had a significantly reduced negative charge compared to that of the other three groups. There were no differences between the SLA-NaCl and SLA-Ca 2+ -NaCl groups in terms of negative charge, and the SLA group surface carried the most negative charge. Fibronectin adsorption capacity and cytological performance testing further showed that the SLA-Ca 2+ group had the most significant change, followed by the SLA-NaCl and SLA-Ca 2+ -NaCl groups; the SLA group had significantly lower capacity and performance than the other three groups. These results suggest that the surface charge of the titanium sheet changed when immersed in different liquids and that this treatment enhanced biocompatibility by reducing the electrostatic repulsion between biomaterials and biomolecules.

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Section: Discussionsupporting

confidence: 77%

Biological Effects of Titanium Surface Charge with a Focus on Protein Adsorption

Ding

et al. 2020

ACS Omega

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“…This demonstrated that the carbon absorbed by the titanium surface is crucial for determining the initial affinity of the osteoblasts, and therefore, the importance of a low C/Ti ratio to increase osteoblastic activity on the titanium surface and thus obtain a higher integration [33]. Other authors, through their research, have obtained similar results [36,37,38,39].…”

Section: Discussionmentioning

confidence: 82%

Superficial Characteristics of Titanium after Treatment of Chorreated Surface, Passive Acid, and Decontamination with Argon Plasma

Rizo-Gorrita

Luna-Oliva

Serrera-Figallo

et al. 2018

JFB

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(1) Background. Titanium is characterized by its biocompatibility, resistance to maximum stress, and fatigue and non-toxicity. The composition, surface structure, and roughness of titanium have a key and direct influence on the osseointegration processes when it is used in the form of dental implants. The objective of the present study is to characterize, at chemical, superficial, and biological levels, the result of the application of the sandblasted with large-grit and acid-etched (SLA) treatment consisting of coarse-grained and double-passivated acid blasting with subsequent decontamination with argon plasma on the surface of titanium implants type IV. (2) Methods. Four Oxtein® dental implants (Zaragoza, Spain) were investigated with the following coding: Code L63713T (titanium grade IV, 3.75 mm in diameter, and 13 mm in length). The surface of the implants was SLA type obtained from coarse-grained, double passivated acid, and decontaminated with argon plasma. The samples were in their sealed packages and were opened in our laboratory. The X-ray photoelectron spectroscopy (XPS) technique was used to characterize the chemical composition of the surface, and the scanning electronic microscope (SEM) technique was used to perform topographic surface evaluation. Cell cultures were also performed on both surfaces. (3) Results. The superficial chemical analysis of the studied samples presented the following components, approximately, expressed in atomic percentage: O: 39%; Ti: 18%; C: 39%; N: 2%; and Si: 1%. In the same way, the topographic analysis values were obtained in the evaluated roughness parameters: Ra: 1.5 μm ± 0.02%; Rq: 1.31 μm ± 0.33; Rz: 8.98 μm ± 0.73; Rp: 5.12 μm ± 0.48; Rv: 3.76 μm ± 0.51; and Rc: 4.92 μm ± 0.24. At a biological level, the expression of osteocalcin was higher (p < 0.05) on the micro-rough surface compared to that machined at 48 and 96 h of culture. (4) Conclusions. The data obtained in our study indicate that the total carbon content, the relative concentration of titanium, and the roughness of the treatment performed on the implants are in agreement with those found in the literature. Further, the roughness of the treatment performed on the implants throws a spongy, three-dimensional surface suitable for bone growth on it. The biological results found are compatible with the clinical use of the surface tested.

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“…However, when the initial healing response indicators such as fibrinogen adsorption and platelet adhesion/spreading were evaluated, there was no significant difference between 1 min and 10 min of UV irradiation; thus, it was concluded that further study was needed. Most other studies have reported that longer irradiation times result in a greater hydrophilic effect by photofunctionalization [ 15 , 23 , 24 , 25 ]. However, for clinical use, it is not appropriate to subject the patient to long treatment times, and hence we set a time of 1 min for this study.…”

Section: Discussionmentioning

confidence: 99%

Effects of Enhanced Hydrophilic Titanium Dioxide-Coated Hydroxyapatite on Bone Regeneration in Rabbit Calvarial Defects

Lee

Bark

Quy

et al. 2018

IJMS

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The regeneration of bone defects caused by periodontal disease or trauma is an important goal. Porous hydroxyapatite (HA) is an osteoconductive graft material. However, the hydrophobic properties of HA can be a disadvantage in the initial healing process. HA can be coated with TiO2 to improve its hydrophilicity, and ultraviolet irradiation (UV) can further increase the hydrophilicity by photofunctionalization. This study was designed to evaluate the effect of 5% TiO2-coated HA on rabbit calvarial defects and compare it with that of photofunctionalization on new bone in the early stage. The following four study groups were established, negative control, HA, TiO2-coated HA, and TiO2-coated HA with UV. The animals were sacrificed and the defects were assessed by radiography as well as histologic and histomorphometric analyses. At 2 and 8 weeks postoperatively, the TiO2-coated HA with UV group and TiO2-coated HA group showed significantly higher percentages of new bone than the control group (p < 0.05). UV irradiation increased the extent of new bone formation, and there was a significant difference between the TiO2-coated HA group and TiO2-coated HA with UV group. The combination of TiO2/HA and UV irradiation in bone regeneration appears to induce a favorable response.

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<b>Enhancing osteoblast-affinity of titanium scaffolds for bone engineering by use of ultraviolet light tr</b><b>eatment </b>

Cited by 13 publications

References 24 publications

Biological Effects of Titanium Surface Charge with a Focus on Protein Adsorption

Biological Effects of Titanium Surface Charge with a Focus on Protein Adsorption

Superficial Characteristics of Titanium after Treatment of Chorreated Surface, Passive Acid, and Decontamination with Argon Plasma

Effects of Enhanced Hydrophilic Titanium Dioxide-Coated Hydroxyapatite on Bone Regeneration in Rabbit Calvarial Defects

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