&lt;b&gt;DIRECT EXTRACTION AND CHARACTERIZATION OF ANIMAL COLLAGENASE FROM HUMAN RADICULAR &lt;/b&gt;&lt;b&gt;CYSTS &lt;/b&gt;

Kawamura, Yasushi; Kishi, Jun-Ichi; Fukaya, Masahiko; Hayakawa, Taro

doi:10.2220/biomedres.8.225

Cited by 2 publications

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“…Fibroblast type collagenase (MMP-l) can also be produced by epithelial cells, monocyte/macrophages and osteoblasts, whereas mature neutrophilic leucocytes are known to release pre-packed and presynthesized proMMP-8 from subcellular specific granules upon different triggering stimuli (12). The present study gives a more detailed characterization of the interstitial collagenase activities previously found to be present in cyst tissues and cell cultures (7)(8)(9)(10). Interstitial collagenases may contribute to jaw cyst expansion at least at two levels; in cyst wall tissue turnover, and in the resorption of the bony wall of the cyst cavity (2,25).…”

Section: Discussionmentioning

confidence: 79%

“…Bone resorption also involves the degradation of extracellular matrix components (2). Several authors have reported fibrinolytic activity in cyst tissue (3)(4)(5)(6) and collagenolytic activity has been shown in vitro in keratocyst tissue (7) as well as in vivo in radicular and keratocyst cyst homogenates (8)(9)(10)(11). However, the type(s) of the interstitial col]agenase(s) participating in the degradation of coUagenous extracellular matrix during the expansion of cysts have not been clarified.…”

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confidence: 99%

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Characterization of interstitial collagenases in jaw cyst wall

Teronen

Salo

Laitinen

et al. 1995

European J Oral Sciences

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Neutral salt extracts of 14 specimens of jaw cysts were prepared. Histopathological analysis showed that the specimens consisted of 6 radicular cysts, 6 dentigerous cysts, 1 residual cyst, and 1 odontogenic keratocyst. One periapical granuloma, 1 dental follicle and a sample of clinically healthy oral mucosa were similarly processed and used as controls. Measurement of collagenase activity by monitoring the formation of specific degradation products of type I and II collagen in solution by SDS-PAGE demonstrated that all the cyst extracts contained collagenase, some of which was endogenously activated. Cyst wall collagenase preferably degraded type I over type II collagen, which suggests that the degradation was due to MMP-1 (matrix metalloproteinase-1) rather than the MMP-8 type. This was further supported by the doxycycline-inhibition profile of cyst collagenase, which was similar to that of MMP-1. Part of the cyst wall collagenase was in latent proenzyme form and probably derived, at least in part, from the newly synthesized intracellular collagenase pool. Latent cyst collagenase was efficiently activated with phenylmercuric chloride and to a lesser extent by gold (I) thioglucose and NaOCl. Western-blotting, using specific antibodies against collagenase from human polymorphonuclear neutrophilic leukocytes (MMP-8) and from fibroblasts (MMP-1), revealed a typical 55/45 kDa doublet; also MMP-8 in the latent 80 kDa form and fragmented to 65 kDa active species were found. These results suggest the presence of MMP-1 and, to a lesser extent, MMP-8 type collagenase in the cyst wall.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 79%

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confidence: 99%