&lt;b&gt;Adipose stem cell sheets improved cardiac function in the rat myocardial infarction, but did not alter cardiac contractile responses to β-adrenergic stim&lt;/b&gt;&lt;b&gt;ulation &lt;/b&gt;

Otsuki, Yuki; Nakamura, Yoshinobu; Harada, Shingo; Yamamoto, Yorihiro; Oginö, Kazuhíde; Morikawa, Kumi; Ninomiya, Haruaki; Miyagawa, Shigeru; Sawa, Yoshiki; Hisatome, Ichiro; Nishimura, Motonobu

doi:10.2220/biomedres.36.11

Cited by 22 publications

(34 citation statements)

References 23 publications

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“…Although these techniques for leakage prevention have various levels of clinical efficacy, they are imperfect solutions that can give burdens for patients. The therapeutic potential of ASC sheets is generally thought to be attributable to ASCs paracrine ability, not the mechanical/physical strength of the ASC sheet to seal the wound [28][29][30]. Although ASC sheets are composed of only a thin layer of cells, it cannot be excluded that the reduction in dehiscence, and abscess formation in this study might be possible due to the mechanical ability of ASC sheets to seal the anastomosis and prevent leakage.…”

Section: █ Discussionmentioning

confidence: 85%

Effects of adipose stem cell sheets on colon anastomotic leakage in an experimental model: Proof of principle

et al. 2017

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The most dreaded complication of colorectal surgery is anastomotic leakage. Adipose tissue-derived stem cell sheets (ASC sheets) prepared from temperature-responsive culture surfaces can be easily transplanted onto tissues. These sheets are proposed to improve cell transplant efficiency and enhance wound healing. The aim of this study was to investigate whether application of ASC sheets could prevent leakage of sutured colorectal anastomoses. Insufficient suturing of colorectal anastomoses was performed in Wistar rats to create a colorectal anastomotic leakage model. Rats were randomized to ASC sheet application or control group. Leakage, abscess formation, adhesion formation, anastomotic bursting pressure (ABP), and histology were evaluated on postoperative day 3 or 7. ASC sheet application significantly reduced anastomotic leakage compared to controls, without increased adhesion formation. ASC sheet transplantation resulted in more CD3+ T-cells and CD163+ anti-inflammatory macrophages at the anastomotic site than the control group. ABP, vessel density and collagen deposition were not different between groups. Using cell sheet technology, we generated ASC sheets that prevented disruption of sutured colorectal anastomoses as shown by reduced leakage. Increased numbers of anti-inflammatory macrophages and T-cells might have contributed to this positive effect.

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Section: █ Discussionmentioning

confidence: 85%

Effects of adipose stem cell sheets on colon anastomotic leakage in an experimental model: Proof of principle

et al. 2017

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“…Different types of trophic factors released by adipose tissue-derived mesenchymal stem cells [27, 65–69, 76, 99]. …”

Section: Figurementioning

confidence: 99%

Trophic Activity and Phenotype of Adipose Tissue-Derived Mesenchymal Stem Cells as a Background of Their Regenerative Potential

Kocan

Maziarz

Tabarkiewicz

et al. 2017

Stem Cells International

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There has been an increased interest in mesenchymal stem cells from adipose tissue, due to their abundance and accessibility with no ethical concerns. Their multipotent properties make them appropriate for regenerative clinical applications. It has been shown that adipose-derived stem cells (ASCs) may differ between the origin sites. Moreover, a variety of internal and external factors may affect their biological characteristics, as what we aimed to highlight in this review. It has been demonstrated that ASCs secrete multiple trophic factors that are capable of stimulating cell proliferation and differentiation and migration of various cell types. Particular attention should be given to exosomes, since it is known that they contribute to the paracrine effects of MSCs. Secretion of trophic agents by ASCs is thought to be in a greater importance for regenerative medicine applications, rather than cells engraftment to the site of injury and their differentiation ability. The surface marker profile of ASCs seems to be similar to that of the mesenchymal stem cells from bone marrow, although some molecular differences are observed. Thus, in this review, we have attempted to define trophic activity, as well as phenotypic characterization of ASCs, as crucial factors for therapeutic usage.

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“…As evidenced by most reports, ADSCs are able to secrete a rich secretome, whereby cell proliferation and differentiation, migration, and an improvement to the cellular and microenvironment protection occurred [7][8][9][10][11][12][13]. This secretome corresponds to a panel of trophic factors, such as cytokines, growth factors, and chemokines, which allow ADSCs to act as paracrine tools that are more likely than cell replacement.…”

Section: Introductionmentioning

confidence: 96%

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Mazini

Rochette

Admou

et al. 2020

IJMS

270

171

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Adipose tissue derived stem cells (ADSCs) are mesenchymal stem cells identified within subcutaneous tissue at the base of the hair follicle (dermal papilla cells), in the dermal sheets (dermal sheet cells), in interfollicular dermis, and in the hypodermis tissue. These cells are expected to play a major role in regulating skin regeneration and aging-associated morphologic disgraces and structural deficits. ADSCs are known to proliferate and differentiate into skin cells to repair damaged or dead cells, but also act by an autocrine and paracrine pathway to activate cell regeneration and the healing process. During wound healing, ADSCs have a great ability in migration to be recruited rapidly into wounded sites added to their differentiation towards dermal fibroblasts (DF), endothelial cells, and keratinocytes. Additionally, ADSCs and DFs are the major sources of the extracellular matrix (ECM) proteins involved in maintaining skin structure and function. Their interactions with skin cells are involved in regulating skin homeostasis and during healing. The evidence suggests that their secretomes ensure: (i) The change in macrophages inflammatory phenotype implicated in the inflammatory phase, (ii) the formation of new blood vessels, thus promoting angiogenesis by increasing endothelial cell differentiation and cell migration, and (iii) the formation of granulation tissues, skin cells, and ECM production, whereby proliferation and remodeling phases occur. These characteristics would be beneficial to therapeutic strategies in wound healing and skin aging and have driven more insights in many clinical investigations. Additionally, it was recently presented as the tool key in the new free-cell therapy in regenerative medicine. Nevertheless, ADSCs fulfill the general accepted criteria for cell-based therapies, but still need further investigations into their efficiency, taking into consideration the host-environment and patient-associated factors.

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<b>Adipose stem cell sheets improved cardiac function in the rat myocardial infarction, but did not alter cardiac contractile responses to β-adrenergic stim</b><b>ulation </b>

Cited by 22 publications

References 23 publications

Effects of adipose stem cell sheets on colon anastomotic leakage in an experimental model: Proof of principle

Effects of adipose stem cell sheets on colon anastomotic leakage in an experimental model: Proof of principle

Trophic Activity and Phenotype of Adipose Tissue-Derived Mesenchymal Stem Cells as a Background of Their Regenerative Potential

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

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