Low-Temperature Three-Dimensional Printing of Tissue Cartilage Engineered with Gelatin Methacrylamide

Luo, Chunyang; Xie, Rui; Zhang, Jiyong; Liu, Yang; Li, Zuxi; Zhang, Yi; Zhang, Xiao; Yuan, Tao; Chen, Yinan; Fan, Weimin

doi:10.1089/ten.tec.2020.0053

Cited by 39 publications

(52 citation statements)

References 41 publications

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“…Among various growth factors, bone morphogenetic protein-2 (BMP-2) is the most potent biomolecule to induce osteogenic differentiation of mesenchymal stem cells (MSCs) in vitro and bone formation in vivo [ 14 , 15 ]. In recent years, cryogenic 3D printing has attracted increasing attention and is being applied to develop bone tissue engineering scaffolds with suitable mechanical properties, biomimetic hierarchical porous structures and in situ delivery of biomolecules, for enhanced cell responses and bone formation in vitro and in vivo [ [16] , [17] , [18] ]. Nevertheless, bone tissue engineering scaffold for clinical use should not only possess excellent osteogenesis for new bone formation but also has interconnected vasculatures for nutrients transfer, oxygen exchange, wastes clearance, cells and signal molecules regulation [ 7 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization

Wang

Lai

et al. 2021

Bioactive Materials

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Three-dimensional (3D) printing has been increasingly employed to produce advanced bone tissue engineering scaffolds with biomimetic structures and matched mechanical strengths, in order to induce improved bone regeneration in defects with a critical size. Given that the successful bone regeneration requires both excellent osteogenesis and vascularization, endowing scaffolds with both strong bone forming ability and favorable angiogenic potential would be highly desirable to induce improved bone regeneration with required vascularization. In this investigation, customized bone tissue engineering scaffolds with balanced osteoconductivity/osteoinductivity were produced via cryogenic 3D printing of β-tricalcium phosphate and osteogenic peptide (OP) containing water/poly(lactic- co -glycolic acid)/dichloromethane emulsion inks. The fabricated scaffolds had a hierarchically porous structure and were mechanically comparable to human cancellous bone. Angiogenic peptide (AP) containing collagen I hydrogel was then coated on scaffold surface to further provide scaffolds with angiogenic capability. A sequential release with a quick AP release and a slow but sustained OP release was obtained for the scaffolds. Both rat endothelial cells (ECs) and rat bone marrow derived mesenchymal stem cells (MSCs) showed high viability on scaffolds. Improved in vitro migration and angiogenesis of ECs were obtained for scaffolds delivered with AP while enhanced osteogenic differentiation was observed in scaffolds containing OP. The in vivo results showed that, toward scaffolds containing both AP and OP, the quick release of AP induced obvious angiogenesis in vivo , while the sustained OP release significantly improved the new bone formation. This study provides a facile method to produce dual-delivery scaffolds to achieve multiple functions.

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Section: Introductionmentioning

confidence: 99%

Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization

Wang

Lai

et al. 2021

Bioactive Materials

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“…Moreover, the hydrogels were shown to elicit the least amount of pro-inflammatory gene expression of macrophages and to inhibit acute immune responses [ 147 ]. It is reported by C. Luo et al that a hybrid low-temperature extrusion-based 3D bioprinting was adopted to deposit cell-laden GelMA hydrogels for cartilage tissue engineering [ 148 ]. It was shown that at a concentration of 5% ( w/v ), the bioprintability (such as sol-gel transition and shear-thinning behavior) of GelMA hydrogels could be improved by changing the deposition temperature.…”

Section: 3d Bioprinting For Medical Applicationsmentioning

confidence: 99%

3D Bioprinting in Tissue Engineering for Medical Applications: The Classic and the Hybrid

et al. 2020

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Three-dimensional (3D) printing, as one of the most popular recent additive manufacturing processes, has shown strong potential for the fabrication of biostructures in the field of tissue engineering, most notably for bones, orthopedic tissues, and associated organs. Desirable biological, structural, and mechanical properties can be achieved for 3D-printed constructs with a proper selection of biomaterials and compatible bioprinting methods, possibly even while combining additive and conventional manufacturing (AM and CM) procedures. However, challenges remain in the need for improved printing resolution (especially at the nanometer level), speed, and biomaterial compatibilities, and a broader range of suitable 3D-printed materials. This review provides an overview of recent advances in the development of 3D bioprinting techniques, particularly new hybrid 3D bioprinting technologies for combining the strengths of both AM and CM, along with a comprehensive set of material selection principles, promising medical applications, and limitations and future prospects.

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“…Due to their potential, BM-MSCs could engineer different layers of native cartilage in vitro. MSCs are usually embedded in a hydrogel to reproduce the hyaline-like cartilaginous matrix, due to their excellent hydration properties, such as alginate [ 162 ] GelMA [ 163 ] or dECM-based bioinks [ 106 ]. An important aspect of tissue mimetism is the fiber organization within the different layers; the bioextrusion process can print layers with different alignments, making it possible to reproduce the collagen fibers’ natural organization within the cartilaginous ECM [ 103 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

“…Very few studies used low cell densities, but they achieved good chondrogenic results [ 154 , 158 ]. For BM-MSCs, the range of densities is comparable to that of chondrocytes, starting from 4 × 10 6 cells/mL [ 175 ] and increasing to as high as 20 × 10 6 cells/mL [ 163 ]; most of the studies were between those two values, thus achieving the best chondrogenic induction possible [ 106 , 162 , 165 , 166 ]. Recently, some research aimed to compare two very low cell densities, 1 and 2 × 10 6 cells/mL, to assess better options for obtaining good chondrogenesis, and they showed that the lowest density seemed to be optimal [ 60 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

“…A comparison of embedded spheroids to isolated cells in bioextruded substitutes demonstrated a better survival of cells inside spheroids [ 175 ]. Globally, the bioextrusion process using biocompatible biomaterials and optimized printing parameters maintains good viability, generally as high as >90% viability, after a sufficient maturation time [ 108 , 118 , 142 , 156 , 163 , 168 , 173 ].…”

Section: Bioextrusion Processes For Cartilage Tissue Engineeringmentioning

confidence: 99%

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Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Messaoudi

Henrionnet

Bourge

et al. 2020

Cells

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Hyaline cartilage is deficient in self-healing properties. The early treatment of focal cartilage lesions is a public health challenge to prevent long-term degradation and the occurrence of osteoarthritis. Cartilage tissue engineering represents a promising alternative to the current insufficient surgical solutions. 3D printing is a thriving technology and offers new possibilities for personalized regenerative medicine. Extrusion-based processes permit the deposition of cell-seeded bioinks, in a layer-by-layer manner, allowing mimicry of the native zonal organization of hyaline cartilage. Mesenchymal stem cells (MSCs) are a promising cell source for cartilage tissue engineering. Originally isolated from bone marrow, they can now be derived from many different cell sources (e.g., synovium, dental pulp, Wharton’s jelly). Their proliferation and differentiation potential are well characterized, and they possess good chondrogenic potential, making them appropriate candidates for cartilage reconstruction. This review summarizes the different sources, origins, and densities of MSCs used in extrusion-based bioprinting (EBB) processes, as alternatives to chondrocytes. The different bioink constituents and their advantages for producing substitutes mimicking healthy hyaline cartilage is also discussed.

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Low-Temperature Three-Dimensional Printing of Tissue Cartilage Engineered with Gelatin Methacrylamide

Cited by 39 publications

References 41 publications

Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization

Cryogenic 3D printing of dual-delivery scaffolds for improved bone regeneration with enhanced vascularization

3D Bioprinting in Tissue Engineering for Medical Applications: The Classic and the Hybrid

Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

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