Low-Temperature Preservation of Human Erythrocytes: Biochemical and Clinical Aspects

Pert, James H.; Schork, P.; Moore, R. H.

doi:10.1159/000426515

Cited by 14 publications

(7 citation statements)

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“…Pert [11] described a method to conserve red cells in the presence of a lower glycerol concentration by rapid freezing with liquid nitro gen. He used an extracellular osmotic gradient of sucrose, to remove glycerol from the red cells.…”

Section: Discussionmentioning

confidence: 99%

Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen

Krijnen¹,

Wit²,

Kuivenhoven³

et al. 1964

Vox Sanguinis

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Section: Discussionmentioning

confidence: 99%

Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen

Krijnen¹,

Wit²,

Kuivenhoven³

et al. 1964

Vox Sanguinis

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“…Currently, there are two methods used clinically for the cryopreservation of RCCs: low glycerol/rapid cooling [298–300] and high glycerol/slow cooling [301, 302]. Low concentrations (15–20%) of glycerol, rapid cooling (>100°C/min), storage in liquid nitrogen (−196°C), or nitrogen vapour (−165°C), with rapid thawing in a 42–45°C water bath, is less commonly used method for clinical cryopreservation of RCCs.…”

Section: Overview: Quality Assessment Of Stored Red Cell Concentratesmentioning

confidence: 99%

Quality Assessment of Established and Emerging Blood Components for Transfusion

Acker

Marks

Sheffield

2016

Journal of Blood Transfusion

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Blood is donated either as whole blood, with subsequent component processing, or through the use of apheresis devices that extract one or more components and return the rest of the donation to the donor. Blood component therapy supplanted whole blood transfusion in industrialized countries in the middle of the twentieth century and remains the standard of care for the majority of patients receiving a transfusion. Traditionally, blood has been processed into three main blood products: red blood cell concentrates; platelet concentrates; and transfusable plasma. Ensuring that these products are of high quality and that they deliver their intended benefits to patients throughout their shelf-life is a complex task. Further complexity has been added with the development of products stored under nonstandard conditions or subjected to additional manufacturing steps (e.g., cryopreserved platelets, irradiated red cells, and lyophilized plasma). Here we review established and emerging methodologies for assessing blood product quality and address controversies and uncertainties in this thriving and active field of investigation.

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“…Two major clinical RBC cryopreservation approaches have been established: the high glycerol/slow freezing [9], [10] and the low glycerol/rapid freezing [11], [12], [13] techniques. The high glycerol/slow freezing technique uses 40% (w/v) glycerol with a cooling rate of ∼1°C/min and storage at −80°C.…”

Section: Introductionmentioning

confidence: 99%

Blood Banking in Living Droplets

et al. 2011

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Blood banking has a broad public health impact influencing millions of lives daily. It could potentially benefit from emerging biopreservation technologies. However, although vitrification has shown advantages over traditional cryopreservation techniques, it has not been incorporated into transfusion medicine mainly due to throughput challenges. Here, we present a scalable method that can vitrify red blood cells in microdroplets. This approach enables the vitrification of large volumes of blood in a short amount of time, and makes it a viable and scalable biotechnology tool for blood cryopreservation.

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Low-Temperature Preservation of Human Erythrocytes: Biochemical and Clinical Aspects

Cited by 14 publications

References 0 publications

Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen

Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen

Quality Assessment of Established and Emerging Blood Components for Transfusion

Blood Banking in Living Droplets

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