Low temperature, pH-triggered synthesis of collagen–chitosan–hydroxyapatite nanocomposites as potential bone grafting substitutes

Effect of Synthesis Temperature on the Crystallization and Growth of In Situ Prepared Nanohydroxyapatite in Chitosan Matrix

“…The height of the peaks at 2 = 32.9 ∘ and 34.6 ∘ decreased and converted into shoulders in case of the composite prepared at −10 ∘ C. This could be ascribed to either a poor crystallinity or a very small crystal size of the produced HA nanoparticles [27,34]. Similar results were presented by Luo et al [36] for collagenchitosan-hydroxyapatite nanocomposites prepared at 4 ∘ C. The prepared composites at higher synthesis temperatures (37 ∘ C and 60 ∘ C) showed similar trends comparing with pure HA. Percentage of crystallinity and crystallite size of HA is expected to increase by increasing the processing temperature [21][22][23]27].…”

Synthesis of highly interconnected 3D scaffold from Arothron stellatus skin collagen for tissue engineering application

“…The triple helix structure of the collagen can be confirmed with respect absorption intensity to amide III and II, which was approximately equal to 1.0, in COL-SPG scaffold as reported by Gordon et al, 1974 andSylvestert et al, 1989 [11-13]. The stretching vibration of collagen at amide A band corresponds to the amide and the peptide group involved in hydrogen bonding interaction [14].…”

Biological evaluation of human hair keratin scaffolds for skin wound repair and regeneration

“…Collagen as one of the most widely used biologically-derived biomaterials has low its immunogenicity and good affinity to cells. Having been safely used in medical fields for decades, collagen-based biomaterials are now well documented in developing various ECM analogues [8,[12][13][14][15][16]. The wide use of other biologically-derived proteins for this purpose also includes fibrin [12], silk fibroin [17] and elastin [18].…”