Constructed wetlands (CWs) are broadly adopted technology
for ecosystem
service and various types of wastewater treatment. However, their
unstable and unpredictable performance hindered their application
and promotion. Transitions in microbial community are strong indicators
of treatment performance of CWs. The unstable treatment of perturbed
CWs is reflected not only in water quality indices but also in altered
aquatic microbial composition. A comprehensive analysis was performed
to elucidate the multistable microbial communities and their relationship
nutrient concentration. Changes in environmental gradients modulate
the strength of microbiota feedbacks and, together with molecular
ecological networks, provide positive feedbacks for the generation
and maintenance of alternative states. The community succession responding
to the concentration of carbon and nitrogen was manipulated by stochastic
instead of deterministic processes. The relative abundance of stochastic
processes increased from state A (73.34%) to state B (89.28%). Furthermore,
the high proportion of stochastic events in microbial communities
easily trigger the transition to alternative states. To summarize,
this study proposes a better understanding of the hypothesis of alternative
stable states in the response of ecosystems to nutrient concentration,
which could provide a strategy for the stoichiometric regulation of
carbon and nitrogen changes along from the inlet to outlet in CWs.