Low Temperature-Induced Cytoplasmic Acidosis in Cultured Mung Bean (Vigna radiata [L.] Wilczek) Cells

Yoshida, Shizuo

doi:10.1104/pp.104.4.1131

Cited by 73 publications

(55 citation statements)

References 22 publications

(29 reference statements)

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“…Transport of these two probes is not prevented by including ammonium acetate in the loading buffer ( Figures 8D and 8H). We have shown that ammonium acetate equilibrates the internal pH of aleurone protein storage vacuoles with that of the external buffer (Swanson and Jones, 1996; see also Yoshida, 1994). Because uptake of BCECF and MCB-GS occurs in the absence of a proton gradient, we conclude that accumulation of these fluorescent probes into aleurone vacuoles is via an ATP-dependent transporter on the tonoplast rather than coupled to a proton-motive force.…”

Section: Fluorescent Probes Are Transported Into Aleurone Vacuoles Bymentioning

confidence: 65%

Barley Aleurone Cells Contain Two Types of Vacuoles: Characterization of Lytic Organelles by Use of Fluorescent Probes

1998

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Light microscopy was used to study the structure and function of vacuoles in living protoplasts of barley ( Hordeum vulgare cv Himalaya) aleurone. Light microscopy showed that aleurone protoplasts contain two distinct types of vacuole: the protein storage vacuole and a lysosome-like organelle, which we have called the secondary vacuole. Fluorescence microscopy using pH-sensitive fluorescent probes and a fluorogenic substrate for cysteine proteases showed that both protein storage vacuoles and secondary vacuoles are acidic, lytic organelles. Ratio imaging showed that the pH of secondary vacuoles was lower in aleurone protoplasts incubated in gibberellic acid than in those incubated in abscisic acid. Uptake of fluorescent probes into intact, isolated protein storage vacuoles and secondary vacuoles required ATP and occurred via at least two types of vanadate-sensitive, ATP-dependent tonoplast transporters. One transporter catalyzed the accumulation of glutathione-conjugated probes, and another transported probes not conjugated to glutathione.

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Section: Fluorescent Probes Are Transported Into Aleurone Vacuoles Bymentioning

confidence: 65%

Barley Aleurone Cells Contain Two Types of Vacuoles: Characterization of Lytic Organelles by Use of Fluorescent Probes

1998

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“…Vacuolar pH (pH,) was equilibrated to that of the externa1 solution by the partitioning effect of weak acid and weak base exchange. Equilibration solutions used for calibration were based on Yoshida (1994) and contained 50 mM Hepes-bis-tris-propane, pH 6.6 to 7.8, or Mes-bis-tris-propane, pH 5.5 to 6.6, 50 mM ammonium acetate, and mannitol to 1100 mosmol kg-'. lmmobilized vacuoles were stepped through pH values by exchanging solution in the chamber with equilibration buffer at a new known pH.…”

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confidence: 99%

Gibberellic Acid Induces Vacuolar Acidification in Barley Aleurone.

Swanson

Jones

1996

Plant Cell

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The roles of gibberellic acid (GA3) and abscisic acid (ABA) in the regulation of vacuolar pH (pH,) in aleurone cells of barley were investigated using the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). BCECF accumulated in vacuoles of aleurone cells, but sequestration of the dye did not affect its sensitivity to pH. BCECFloaded aleurone cells retained their ability to respond to both GA3 and ABA. The pH, of freshly isolated aleurone cells is 6.6, but after incubation in GA3, the pH, fel1 to 5.8. The pH, of cells not incubated in hormones or in the presence of ABA showed little orno acidification. The aleurone tonoplast contains both vacuolar ATPase and vacuolar pyrophosphatase, but the levels of pump proteins were not affected by incubation in the presence or absence of hormones. We conclude that GA3 affects the pH, in aleurone cells by altering the activities of tonoplast H+ pumps but not the amounts of pump proteins.

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“…Thus, the phase transition of the membranes was thought to be one of the primary causes of chilling injury. [11][12][13] Article Addendum Low temperature acclimation mediated by ethanol production is essential for chilling tolerance in rice roots Hisashi Kato-Noguchi Department of Applied Biological Science; Faculty of Agriculture; Kagawa University; Miki, Kagawa, Japan Abbreviations: ADH, alcohol dehydrogenase; LP-PT, low temperature pretreatment; HSP, heat shock protein …”

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confidence: 99%

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confidence: 99%

Low temperature acclimation mediated by ethanol production is essential for chilling tolerance in rice roots

Kato‐Noguchi

2008

Plant Signaling & Behavior

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Rice seedlings (Oryza sativa L.) were subjected to low temperature pretreatment (LT-PT; 10˚C) for various length of time followed by a 48-h chilling temperature stress (2˚C). Chilling tolerance of rice roots was improved with increasing duration of LT-PT, but HT-PT longer than 12 h gave no additional improvement. LT-PT did not change in fatty acid composition in rice roots under the present experimental condition. Alcohol dehydrogenase (ADH) activity and ethanol concentration in the roots were increased with increasing duration of LT-PT up to 12 h, which indicates that LT-PT increased ethanol fermentation in the roots. 4-Methylpyrazole, a potent inhibitor of ADH, reduced the ethanol concentration and the chilling tolerance in the roots. This reduction of the chilling tolerance recovered with exogenously applied ethanol. Ethanol also induced 21-and 33-kD protein synthesis in the roots and these proteins may contribute the improvement of the tolerance. The present research suggests that LT-PT may increase chilling tolerance in rice roots owing to ethanol production, and ethanol may trigger a signal transduction cascade, which might lead to a decrease in membrane damage and injury.Alcohol dehydrogenase (ADH; EC.1.1.1.1) gene and protein were induced by low temperature in Arabidopsis, maize and rice seedlings. 1,2,3 ADH is an enzyme involved in ethanolic fermentation and essential for plants to survive under anaerobic conditions. 4,5 However, it is unlikely that the induction of ADH by low temperture is due to a switch from aerobic respiration to anaerobic respiration as reported with anaerobic conditions. 2,6 Therefore, it is not clear that biological meanings of the induction of ADH in low temperature conditions.Rice seedlings (Oryza sativa L. cv. Nipponbare) were subjected to low temperature pretreatment (LT-PT; 10˚C) for various length of time (1, 2, 4, 6, 12, 18, 24 h) followed by a 48-h chilling temperature stress (2˚C). Chilling tolerance of rice roots was improved with increasing duration of LT-PT, but HT-PT longer than 12 h gave no additional improvement. LT-PT did not change in any fatty acid compositions in rice roots under the present experimental condition. Several plant species, such as oat, rye and spinach increased freezing tolerance due to the increasing unsaturation of fatty acids in plasma membranes, but this cold acclimation process required exposure of these plants to subzero temperature for 2-3 weeks. 7,8 LT-PT increased ADH activity and ethanol concentration in rice roots, and the activity and the concentration were increased with increasing duration of LT-PT up to 12 h. Thus, LT-PT induced ethanolic fermentation system and stimulated ethanol production in the roots. 4-Methylpyrazole, which is a potent inhibitor of ADH and prevents ethanol production, 9 reduced rice root growth to 40% of LP-PT root growth (Fig. 1), and the ethanol accumulation in the roots. This growth inhibition by 4-methylpyrazole recovered with exogenously applied ethanol. These results suggest that ethanol produced by LT-P...

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Low Temperature-Induced Cytoplasmic Acidosis in Cultured Mung Bean (Vigna radiata [L.] Wilczek) Cells

Abstract: Cold-induced changes in vivo in

Cited by 73 publications

References 22 publications

Barley Aleurone Cells Contain Two Types of Vacuoles: Characterization of Lytic Organelles by Use of Fluorescent Probes

Barley Aleurone Cells Contain Two Types of Vacuoles: Characterization of Lytic Organelles by Use of Fluorescent Probes

Gibberellic Acid Induces Vacuolar Acidification in Barley Aleurone.

Low temperature acclimation mediated by ethanol production is essential for chilling tolerance in rice roots

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