Low Temperature Effects on Anaerobic Microbial Kinetic Parameters

Banik, Gouranga; Viraraghavan, T.; Dague, Richard R.

doi:10.1080/09593331908616706

Cited by 18 publications

(6 citation statements)

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“…To elucidate the hydrolytic capacity of the microbial biomass, particularly with respect to protein degradation, tests using skimmed milk as a protein source were performed and various kinetic parameters ( A max , K m , and k ) were calculated. The half-saturation constant K m was higher at 12°C than at 37°C throughout the trial ( Table 4 ) indicating that microorganisms with lower substrate affinity predominated at the lower temperature, which is in agreement with a previous report ( Banik et al, 1998 ). By the end of Phase 1, the K m at 37 and 12°C had doubled compared to the K m of the seed biomass, again an indication of a decrease in the substrate affinity, presumably as a response to the adaptation to cold temperatures ( Table 4 ).…”

Section: Resultssupporting

confidence: 92%

Biological Phosphorus Removal During High-Rate, Low-Temperature, Anaerobic Digestion of Wastewater

et al. 2016

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We report, for the first time, extensive biologically mediated phosphate removal from wastewater during high-rate anaerobic digestion (AD). A hybrid sludge bed/fixed-film (packed pumice stone) reactor was employed for low-temperature (12°C) anaerobic treatment of synthetic sewage wastewater. Successful phosphate removal from the wastewater (up to 78% of influent phosphate) was observed, mediated by biofilms in the reactor. Scanning electron microscopy and energy dispersive X-ray analysis revealed the accumulation of elemental phosphorus (∼2%) within the sludge bed and fixed-film biofilms. 4′, 6-diamidino-2-phenylindole (DAPI) staining indicated phosphorus accumulation was biological in nature and mediated through the formation of intracellular inorganic polyphosphate (polyP) granules within these biofilms. DAPI staining further indicated that polyP accumulation was rarely associated with free cells. Efficient and consistent chemical oxygen demand (COD) removal was recorded, throughout the 732-day trial, at applied organic loading rates between 0.4 and 1.5 kg COD m-3 d-1 and hydraulic retention times of 8–24 h, while phosphate removal efficiency ranged from 28 to 78% on average per phase. Analysis of protein hydrolysis kinetics and the methanogenic activity profiles of the biomass revealed the development, at 12°C, of active hydrolytic and methanogenic populations. Temporal microbial changes were monitored using Illumina MiSeq analysis of bacterial and archaeal 16S rRNA gene sequences. The dominant bacterial phyla present in the biomass at the conclusion of the trial were the Proteobacteria and Firmicutes and the dominant archaeal genus was Methanosaeta. Trichococcus and Flavobacterium populations, previously associated with low temperature protein degradation, developed in the reactor biomass. The presence of previously characterized polyphosphate accumulating organisms (PAOs) such as Rhodocyclus, Chromatiales, Actinobacter, and Acinetobacter was recorded at low numbers. However, it is unknown as yet if these were responsible for the luxury polyP uptake observed in this system. The possibility of efficient phosphate removal and recovery from wastewater during AD would represent a major advance in the scope for widespread application of anaerobic wastewater treatment technologies.

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Section: Resultssupporting

confidence: 92%

Biological Phosphorus Removal During High-Rate, Low-Temperature, Anaerobic Digestion of Wastewater

et al. 2016

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“…Many investigations related to the effect of temperature on anaerobic digestion show a very strong negative effect on the metabolic activity of the anaerobic microorganisms with temperature decrease [8] because like other biological processes, anaerobic digestion depends strongly on temperature. Generally speaking, the higher the temperature leads, the higher the microbial activity up to an optimum temperature [9].…”

Section: Introductionmentioning

confidence: 99%

A novel measurement method of temperature model for bioreactor

Xuan

Shao

2010

Front. Electr. Electron. Eng. China

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A novel measurement method of temperature model for bioreactor has been proposed. Temperature is the key parameter in monitoring the bioreactor operation. However, the system input signal of bioreactor is delayed, and model parameters are uncertain, so the output of temperature is non-steady-state. Many dynamic measurements are not steady so that it cannot be described by variables constant in time. In this paper, we adopt the monopulse signal as input so that the output of the bioreactor system is steady. This method has a powerful ability to steady the output of the bioreactor. In view of the measurement results, it can be seen that the model dynamic measurement approaches the real process. The analytical expression of the monopulse response for the temperature model of the bioreactor is obtained. The novel measurement approach is simple and can be easily adopted by industry.

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“…Palm oil mill effluent (POME) is a possible substrate to be used for dark fermentation due to the high compositions and concentrations of carbohydrates, proteins, nitrogenous compounds, lipids, and minerals [6,7]. Until *Corresponding author: jamal@ukm.edu.my 2011, 55 out of 426 palm oil mills in Malaysia had installed biogas plants [8] and 43.29 million cubic meters per year of POME were generated during milling process based on calculation from 2.5 m 3 raw POME generated [9].…”

Section: Introductionmentioning

confidence: 99%

Kinetic Model of Thermophilic Biohydrogen Production from POME

Jahim

Abdul

Chu

et al. 2019

IJIE

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The study of fermentation kinetic parameters are crucial to understand the environmental factors affect on biohydrogen production. Kinetic models for hydrogen production from anaerobic digestion of palm oil mill effluent (POME) by mixed culture were developed based on published work. The models accounted for substrate limitation, substrate inhibition, hydrogen production, and endogenous decay rate. Data from previous literature were used to compare four microbial growth kinetic models for hydrogen production in an ASBR system. The estimated values of the maximum specific growth rate (μm) were found to be 0.371 h-1. In this study, the parameters of Y, kd, and B0 calculated were 2.64 gVSS/gCOD, 0.053 h-1 , and 0.133 L H2/gCOD, respectively. The model fitting was found to be in good agreement with the experimental and can be utilized for the optimization and design of the process.

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Low Temperature Effects on Anaerobic Microbial Kinetic Parameters

Cited by 18 publications

References 0 publications

Biological Phosphorus Removal During High-Rate, Low-Temperature, Anaerobic Digestion of Wastewater

Biological Phosphorus Removal During High-Rate, Low-Temperature, Anaerobic Digestion of Wastewater

A novel measurement method of temperature model for bioreactor

Kinetic Model of Thermophilic Biohydrogen Production from POME

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