Low serum testosterone assayed by liquid chromatography-tandem mass spectrometry. Comparison with five immunoassay techniques

Moal, Valérie; Mathieu, E; Reynier, Pascal; Malthièry, Yves; Gallois, Yves

doi:10.1016/j.cca.2007.07.013

Cited by 136 publications

(90 citation statements)

References 14 publications

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“…We are cautious to generalize the agreement between extracted RIA and LC-MS/MS in the current study, because only two centers participated in the current study. But the results are in accordance with previous studies (36,37,39), including a study on women with PCOS, which confirms that well-chosen RIA may offer equal precision and accuracy like LC-MS/MS (40).…”

Section: Discussionsupporting

confidence: 91%

“…A study comparing LC-MS/MS with direct and extraction RIAs in normal and hypogonadal male subjects by Bland-Altman plots showed systematically lower results by LC-MS/MS (36). Studies on female and pediatric samples confirmed this finding, while also identifying that LC-MS/MS showed greater accuracy in lower androgen concentrations than direct assays (37,38). This study included serum samples of women with PCOS, POI, and normally cyclic women.…”

Section: Discussionmentioning

confidence: 66%

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Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

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Objective: The measurement of serum testosterone in women is challenging due to lack of trueness, precision, and sensitivity of various available testosterone assays. Accurate assessment of testosterone in women is crucial especially in conditions associated with alleged over-or under-production of testosterone, such as in polycystic ovary syndrome (PCOS) or primary ovarian insufficiency (POI). The aim of this study was to measure and compare androgen concentrations in women with PCOS, POI, and female controls and to evaluate the performance of extraction RIA and liquid chromatographytandem mass spectrometry (LC-MS/MS) in these women. Design: Cross-sectional study. Methods: Carefully phenotyped women with POI (nZ208) or PCOS (nZ200) and 45 healthy, regularly cyclic female controls were included. Method comparison analyses were performed for total testosterone, androstenedione (AD), and DHEA, as measured by LC-MS/MS and extraction RIA. Results: All androgen levels were significantly elevated in women with PCOS compared with POI patients (P!0.05) and controls (P!0.05). Women with POI presented with similar androgen concentrations as controls, except for AD. Compared with measurements by extraction RIA, testosterone, DHEA, and AD concentrations measured by LC-MS/MS were systematically lower. However, using extraction RIA and LC-MS/MS, testosterone, DHEA, and AD measurements were shown to have good agreement as assessed by Bland-Altman analysis and intraclass correlation coefficient: 0.95 (95% confidence interval 0. 94-0.91), 0.83 (0.79-0.86), and 0.96 (0.95-0.97) respectively. Conclusions: LC-MS/MS, compared with a labor-intensive extraction RIA, shows good precision, sensitivity, and high accuracy for measuring female testosterone, DHEA, and AD concentrations under various clinical conditions. LC-MS/MS, therefore, represents a convenient and reliable assay for both clinical and research purposes, where androgen measurement in women is required. European Journal of Endocrinology 165 925-933

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 66%

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Janse

Eijkemans

Goverde

et al. 2011

European Journal of Endocrinology

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“…saliva (Gröschl et al, 2001). Steroid hormone immunoassays are often not consistent, especially at normal and low concentrations (Moal et al, 2007) because of low assay specificity, inadequate standardization, and poor optimization of the methods over the large range of concentrations seen clinically (Taieb et al, 2002).…”

Section: Steroid Analysismentioning

confidence: 99%

“…Different techniques are used (see Table 1), such as liquid-liquid extraction (Moal et al, 2007) and solid phase extraction (Herold and Fitzgerald, 2003), normally with a protein precipitation step beforehand. The aim in a routine clinical laboratory should be rapid pre-analytical sample processing with minimal manual sample manipulation, providing the desired sensitivity and high specificity.…”

Section: A N U S C R I P Tmentioning

confidence: 99%

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Steroid measurement with LC–MS/MS in pediatric endocrinology

Rauh

2009

Molecular and Cellular Endocrinology

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Please cite this article as: Rauh, M., Steroid measurement with LC-MS/MS in pediatric endocrinology, Molecular and Cellular Endocrinology (2008), doi:10.1016/j.mce.2008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Review of electrochemical and optical biosensors for testosterone measurement

Nikkhah

Karami

Khatami

et al. 2022

Biotech and App Biochem

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Testosterone is an anabolic steroid and a major sex hormone in males. It plays vital roles, including developing the testis, penis, and prostate, increasing muscle and bone, and sperm production. In both men and women, testosterone levels should be in normal ranges. Besides, testosterone and its analogs are major global contributors to doping in sport. Due to the importance of testosterone testing, novel, accurate biosensors have been developed. This review summarizes the various methods for testosterone measurement. Also, recent optical and electrochemical approaches for the detection of testosterone and its analogs have been discussed.

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Low serum testosterone assayed by liquid chromatography-tandem mass spectrometry. Comparison with five immunoassay techniques

Cited by 136 publications

References 14 publications

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Assessment of androgen concentration in women: liquid chromatography–tandem mass spectrometry and extraction RIA show comparable results

Steroid measurement with LC–MS/MS in pediatric endocrinology

Review of electrochemical and optical biosensors for testosterone measurement

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