Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate

Wu, Junyi; Chen, Chia-Hsin; Yeh, Lo-Yao; Yeh, Ming Long; Ting, Chun Chan; Wang, Yan‐Hsiung

doi:10.1038/ijos.2013.38

Cited by 75 publications

(55 citation statements)

References 38 publications

(41 reference statements)

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“…Reverse transcription PCR was performed using Primescript RT reagent Kit (TaKaRa), and further quantitative real-time PCR analysis was performed using SYBR select master mix (Applied Biosystems; TCAB1 primers: Forward, 5’-CATATCTGGGACGCATTCACT-3’, Reverse, 5’-GTTGAAGCCACAGAAGAGCTG-3’). The PCR results were quantified using semi-quantitative method as previously reported [22]. …”

Section: Methodsmentioning

confidence: 99%

TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas

et al. 2014

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BackgroundWRAP53, including α, β and γ isoforms, plays an important role not only in the stability of p53 mRNA, but also in the assembly and trafficking of the telomerase holoenzyme. It has been considered an oncogene and is thought to promote the survival of cancer cells. The aim of this study was to detect the role of TCAB1 (except WRAP53α) in the occurrence and development of head and neck carcinomas.MethodsImmunohistochemistry was used to detect the TCAB1 expression in clinical specimen sections and performed western blotting to check the TCAB1 expression levels in cell lines. TCAB1 was depleted using shRNA lentivirus and the knockdown efficiency was assessed using q-PCR and Western blotting. We performed CCK-8 assays and flow cytometry to check the cell proliferation potential and used the trans-well assay to test the invasion ability in vitro. Xenografts were used to detect the tumor formation potential in vivo. Moreover, we performed cDNA microarray to investigate the candidate factors involved in this process.ResultsWe observed a notable overexpression of TCAB1 in head and neck carcinoma clinical specimens as well as in carcinoma cell lines. Knockdown of TCAB1 decreased the cellular proliferation potential and invasion ability in vitro. cDNA microarray analysis suggested the possible involvement of several pathways and factors associated with tumorigenesis and carcinoma development in the TCAB1-mediated regulation of cancers. Furthermore, the xenograft assay confirmed that the depletion of TCAB1 would inhibit tumor formation in nude mice. The immunohistochemistry results of the mice tumor tissue sections revealed that the cells in shTCAB1 xenografts showed decreased proliferation potential and increased apoptotic trend, meanwhile, the angiogenesis was inhibited in the smaller tumors form shTCAB1 cells.ConclusionsOur study demonstrated that depletion of TCAB1 decreased cellular proliferation and invasion potential both in vitro and in vivo. The data indicated that TCAB1 might facilitate the occurrence and development of head and neck carcinomas. In future, TCAB1 might be useful as a prognostic biomarker or a potential target for the diagnosis and therapy of head and neck carcinomas.

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Section: Methodsmentioning

confidence: 99%

TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas

et al. 2014

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“…Kinin receptors Down-regulates kinin receptor expression in neutrophils of patients undergoing heart surgery. (Saxena et al, 2013) Downregulates kinin receptors mRNA expression in the subplantar muscle of rat paw (Bortone et al, 2008) Ion channels K(+) inward rectifier subunits of the 6.2 isotype (Kir6.2) required for cardioprotection (Wojtovich et al, 2013) Increased Ca2+ in oral tissue cells via TRPC1 ion channels (Chellini et al, 2010) Cyclic AMP/CREB CREB phosphorylation contributes to triggering preconditioning in rat heart (Marais et al, 2008) Promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic AMP (Wu et al, 2013) VEGF Overexpression of VEGF protects intrahepatic cholangiocytes after liver transplantation in rats (Zhou et al, 2010) Increases angiogenesis in a model of ischemic skin flap in rats mediated by VEGF, HIF-1α and MMP-2 (Cury et al, 2013) Hypoxia-inducible factor 1 HIF-1 activates IL10 gene transcription and is required for remote ischemic preconditioning in mice heart (Cai et al, 2013;Kalakech et al, 2013) Reduces the overexpressions of HIF-1α, TNF-α, and IL-1β, and increases the amounts of VEGF, NGF, and S100 proteins in rats with chronic constriction injury (Hsieh et al, 2012) TFG-beta Downregulated TGFβ1 but upregulated smad7 in cardiac infarcts Increase in proliferation of vascular endothelial cells and decrease in VEGF and TGF-beta secretion. (Szymanska et al, 2013) …continued mechanisms is hypothesized to be at the level of mitochondria (Dirnagl and Meisel, 2008).…”

Section: Similarities Between Ipc and Llltmentioning

confidence: 99%

Pre-Conditioning with Low-Level Laser (Light) Therapy: Light before the Storm

et al. 2014

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h Pre-conditioning by ischemia, hyperthermia, hypothermia, hyperbaric oxygen (and numerous other modalities) is a rapidly growing area of investigation that is used in pathological conditions where tissue damage may be expected. The damage caused by surgery, heart attack, or stroke can be mitigated by pre-treating the local or distant tissue with low levels of a stress-inducing stimulus, that can induce a protective response against subsequent major damage. Low-level laser (light) therapy (LLLT) has been used for nearly 50 years to enhance tissue healing and to relieve pain, inflammation and swelling. The photons are absorbed in cytochrome(c) oxidase (unit four in the mitochondrial respiratory chain), and this enzyme activation increases electron transport, respiration, oxygen consumption and ATP production. A complex signaling cascade is initiated leading to activation of transcription factors and up-and down-regulation of numerous genes. Recently it has become apparent that LLLT can also be effective if delivered to normal cells or tissue before the actual insult or trauma, in a pre-conditioning mode. Muscles are protected, nerves feel less pain, and LLLT can protect against a subsequent heart attack. These examples point the way to wider use of LLLT as a pre-conditioning modality to prevent pain and increase healing after surgical/medical procedures and possibly to increase athletic performance.

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“…The second route involves the well-known ability of PBM to increase the expression levels of cAMP [90–92]. As mentioned above, cAMP activates protein kinase A which then phopsphorylates AANAT thus preserving its enzyme bioactivity and increasing melatonin synthesis.…”

mentioning

confidence: 99%

Aging of lymphoid organs: Can photobiomodulation reverse age‐associated thymic involution via stimulation of extrapineal melatonin synthesis and bone marrow stem cells?

Odinokov

Hamblin

2018

Journal of Biophotonics

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Thymic atrophy and the subsequent reduction in T-cell production are the most noticeable age-related changes affecting lymphoid organs in the immune system. In fact, thymic involution has been described as "programmed aging." New therapeutic approaches, such as photobiomodulation (PBM), may reduce or reverse these changes. PBM (also known as low-level laser therapy) involves the delivery of non-thermal levels of red or near-infrared light that are absorbed by mitochondrial chromophores, in order to prevent tissue death and stimulate healing and regeneration. PBM may reverse or prevent thymic involution due to its ability to induce extrapineal melatonin biosynthesis via cyclic adenosine monophosphate (AMP) or NF-kB activation, or alternatively by stimulating bone marrow stem cells that can regenerate the thymus. This perspective puts forward a hypothesis that PBM can alter thymic involution, improve immune functioning in aged people and even extend lifespan.

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Low-power laser irradiation promotes the proliferation and osteogenic differentiation of human periodontal ligament cells via cyclic adenosine monophosphate

Cited by 75 publications

References 38 publications

TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas

TCAB1: a potential target for diagnosis and therapy of head and neck carcinomas

Pre-Conditioning with Low-Level Laser (Light) Therapy: Light before the Storm

Aging of lymphoid organs: Can photobiomodulation reverse age‐associated thymic involution via stimulation of extrapineal melatonin synthesis and bone marrow stem cells?

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