Low‐Potential Detection of Glucose with a Biosensor Based on the Immobilization of Glucose Oxidase on Polymer/Manganese Oxide Layered Nanocomposite

Abstract

A nanocomposite with poly(diallyldimethylammonium), PDDA, intercalated between manganese oxide layers is constructed on a graphite electrode surface through one-step electrodeposition and used to adsorb glucose oxidase (GOD). The immobilized GOD displays a pair of stable and quasireversible redox peaks with a formal potential of À 468 mV in pH 7.0 buffer solutions and exhibits excellent electrocatalysis to the reduction of oxygen. In the presence of dissolved oxygen, the reduction peak current decreased gradually with the addition of glucose, indicating that the immobilized GOD kept its bioactivity. Thus a reagentless biosensor for glucose at a low detection potential was established. The linear concentration range is from 0.02 to 2.78 mM with a detection limit of 9.8 mM. The proposed glucose biosensor was insensitive to common interferences such as ascorbic and uric acids etc.

A high performance nonenzymatic electrochemical glucose sensor based on polyvinylpyrrolidone–graphene nanosheets–nickel nanoparticles–chitosan nanocomposite

“…Due to the observably increasing request in diabetes clinical diagnostics [1] and the requirements in the food industry [2] and life science [3], the development of accurate, reliable and low cost devices for glucose detection becomes one of the most important scientific focuses. Since Updike and Hicks [4] detected glucose concentration in biological fluids for the first time by immobilizing glucose oxidase (GOD) on oxygen electrode in 1967, a great number of biosensors based on the GOD have been investigated [5][6][7][8]. These sensors could obtain high sensitivity and selectivity.…”

Co-electrodeposited rGO/MnO2 nanohybrid for arsenite detection in water by stripping voltammetry

“…Manganese oxide materials have been extensively investigated in the past and were applied in a wide range of fields, such as electrochemical super-capacitors, biosensors, lithium ion batteries, etc [11][12][13][14]. In recent years, it has also been reported for removal and detection of arsenic in water due to its stability and excellent adsorption capability towards arsenic [15][16][17][18].…”

A Biosensor Composed of Glucose Oxidase‐Containing Liposomes and MnO2‐Based Layered Nanocomposite

“…In such applications, manganese oxides are often employed by mixing with conductive carbon paste [1,3,6]. Otherwise, thin films of manganese oxides are prepared by casting [2,4], electrostatic layer-bylayer (LBL) assembly [5], and electrodeposition [7]. Furthermore, nanostructures of manganese oxides can enhance their electrochemical properties as a result of reduced diffusion paths and increased accessible active sites.…”

“…Enzyme-based biosensors are usually fabricated by immobilizing enzymes to the surface of an electrode. If an enzyme such as glucose oxidase (GOD) can be incorporated into a matrix that can act as an electron mediator for the oxidation of H 2 O 2 , the resulting electrode functions as a biosensor such as glucose sensor [6,7]. In such applications, manganese oxides are often employed by mixing with conductive carbon paste [1,3,6].…”