A Biosensor Composed of Glucose Oxidase‐Containing Liposomes and MnO<sub>2</sub>‐Based Layered Nanocomposite

Yoshimoto, Makoto; Iida, Chihiro; Kariya, Asuka; Takaki, Noriyuki; Nakayama, Masaharu

doi:10.1002/elan.200900413

Cited by 11 publications

(4 citation statements)

References 16 publications

(25 reference statements)

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“…Anionic and zwitterionic phospholipids [1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3phosphocholine (POPC); N-glutaryl-phosphatidylethanolamine (NGPE); 1,2dihexadecanoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (DPPG); 1hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (POPG); 1,2-dimyristoyl-sn-glycero-3-phospho-(1-rac-glycerol) (DMPG); lyso-1heptadecanoyl-sn-glycero-3-phosphocholine (LPC)] and Langmuir-Blodgett films composed by arachidic acid have been the most tested for the development of enzymatic biosensors (Table 1); the only enzymes used on those studies were tyrosinase (Tyr), MP, monoamine oxidase B (MAO-B), HRP, acetylcholinesterase (AChE) and glucose oxidase (GOD). Still, the number of studies (Table 1) is very limited [ [24], [25], [26], [27], [28], [29], [30]]. Cationic and other zwitterionic phospholipids, such as 1,2-di-(9Zoctadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-di-(9Z-octadecenoyl)-3trimethylammonium-propane (DOTAP) and 1,2-distearoyl-sn-glycero-3phosphoethanolamine (DSPE) have not yet been tested for preservation, stabilization and immobilization of enzymes.…”

Section: Introductionmentioning

confidence: 99%

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Gomes

Maia

Loureiro

et al. 2019

Bioelectrochemistry

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An enzymatic biosensor based on nitric oxide reductase (NOR; purified from Marinobacter hydrocarbonoclasticus) was developed for nitric oxide (NO) detection. The biosensor was prepared by deposition onto a pyrolytic graphite electrode (PGE) of a nanocomposite constituted by carboxylated single-walled carbon nanotubes (SWCNTs), a lipidic bilayer [1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-di-(9Z-octadecenoyl)-3-trimethylammonium-propane (DOTAP), 1,2-distearoyl-snglycero-3-phosphoethanolamine-polyethylene glycol (DSPE-PEG)] and NOR. NOR direct electron transfer and NO bioelectrocatalysis were characterized by several electrochemical techniques. The biosensor development was also followed by scanning electron microscopy and Fourier transform infrared spectroscopy. Improved enzyme stability and electron transfer (1.96 × 10 −4 cm.s −1 apparent rate constant) was obtained with the optimum SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR) ratio of 4/2.5/4 (v/v/v), which biomimicked the NOR environment. The PGE/[SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR] biosensor exhibited a low Michaelis-Menten constant (4.3 μM), wide linear range (0.44-9.09 μM), low detection limit (0.13 μM), high repeatability (4.1% RSD), reproducibility (7.0% RSD), and stability (ca. 5 weeks). Selectivity tests towards Larginine, ascorbic acid, sodium nitrate, sodium nitrite and glucose showed that these compounds did not significantly interfere in NO biosensing (91.0 ± 9.3%-98.4 ± 5.3% recoveries). The proposed biosensor, by incorporating the benefits of biomimetic features of the phospholipid bilayer with SWCNT's inherent properties and NOR bioelectrocatalytic activity and selectivity, is a promising tool for NO.

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Section: Introductionmentioning

confidence: 99%

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Gomes

Maia

Loureiro

et al. 2019

Bioelectrochemistry

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“…However, the lipid counterpart of the polymersomes, named “liposomes”, has already found a place in biosensing applications as signal amplifiers or as biorecognition elements . For instance, encapsulation of signaling molecules such as enzymes, fluorescent markers, and electrochemical markers have been already utilized in liposome-based biosensors for the detection of nucleic acids, antibodies, and large biomacromolecules . In addition, such (macro)molecules can also be conjugated to the surface of the vesicles to realize bio/chemo recognition in microsystem devices .…”

Section: Introductionmentioning

confidence: 99%

Immobilized Multifunctional Polymersomes on Solid Surfaces: Infrared Light-Induced Selective Photochemical Reactions, pH Responsive Behavior, and Probing Mechanical Properties under Liquid Phase

İyisan

Janke

Reichenbach³

et al. 2016

ACS Appl. Mater. Interfaces

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Fixing polymersomes onto surfaces is in high demand not only for the characterization with advanced microscopy techniques but also for designing specific compartments in microsystem devices in the scope of nanobiotechnology. For this purpose, this study reports the immobilization of multifunctional, responsive, and photo-cross-linked polymersomes on solid substrates by utilizing strong adamantane-β-cyclodextrin host-guest interactions. To reduce nonspecific binding and retain better spherical shape, the level of attractive forces acting on the immobilized polymersomes was tuned through poly(ethylene glycol) passivation as well as decreased β-cyclodextrin content on the corresponding substrates. One significant feature of this system is the pH responsivity of the polymersomes which has been demonstrated by swelling of the immobilized vesicles at acidic condition through in situ AFM measurements. Also, light responsivity has been provided by introducing nitroveratryloxycarbonyl (NVOC) protected amine molecules as photocleavable groups to the polymersome surface before immobilization. The subsequent low-energy femtosecond pulsed laser irradiation resulted in the cleavage of NVOC groups on immobilized polymersomes which in turn led to free amino groups as an additional functionality. The freed amines were further conjugated with a fluorescent dye having an activated ester that illustrates the concept of bio/chemo recognition for a potential binding of biological compounds. In addition to the responsive nature, the mechanical stability of the analyzed polymersomes was supported by computing Young's modulus and bending modulus of the membrane through force curves obtained by atomic force microscopy measurements. Overall, polymersomes with a robust and pH-swellable membrane combined with effective light responsive behavior are promising tools to design smart and stable compartments on surfaces for the development of microsystem devices such as chemo/biosensors.

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“…Besides, has also been widely used in biosensors. [25][26][27][28] However, the application of MnO 2 was restricted by its poor electrical conductivity.…”

Section: -24mentioning

confidence: 99%

An amperometric glucose biosensor based on a MnO₂/graphene composite modified electrode

Liu

Zhang

et al. 2016

RSC Adv.

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In this paper, a novel composite of graphene/MnO 2 (GR/MnO 2 ) was successfully synthesized by a simple one-step hydrothermal method. The as-synthesized MnO 2 and the composite were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). The results showed that MnO 2 was nanorods and the two materials were perfectly composited. The composite was decorated on a glassy carbon electrode (GCE) and used for the entrapment of glucose oxidase (GOD). Electrochemical results showed that the composite modified electrode showed a pair of well-defined redox peaks, and the direct electron transfer between GOD and the electrode surface was accelerated. The sensor fabricated by the composite modified electrode showed an excellent response to the oxidation of glucose with a wide linear range (0.04 to 2 mM), low detection limit (10 mM), and high sensitivity (3.3 mA mM À1 cm À2). The sensor also exhibited excellent reproducibility, stability and selectivity, and it can be used in the determination of glucose in real samples.

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A Biosensor Composed of Glucose Oxidase‐Containing Liposomes and MnO₂‐Based Layered Nanocomposite

Cited by 11 publications

References 16 publications

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Immobilized Multifunctional Polymersomes on Solid Surfaces: Infrared Light-Induced Selective Photochemical Reactions, pH Responsive Behavior, and Probing Mechanical Properties under Liquid Phase

An amperometric glucose biosensor based on a MnO₂/graphene composite modified electrode

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A Biosensor Composed of Glucose Oxidase‐Containing Liposomes and MnO2‐Based Layered Nanocomposite

Cited by 11 publications

References 16 publications

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite

Immobilized Multifunctional Polymersomes on Solid Surfaces: Infrared Light-Induced Selective Photochemical Reactions, pH Responsive Behavior, and Probing Mechanical Properties under Liquid Phase

An amperometric glucose biosensor based on a MnO2/graphene composite modified electrode

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Product

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A Biosensor Composed of Glucose Oxidase‐Containing Liposomes and MnO₂‐Based Layered Nanocomposite

An amperometric glucose biosensor based on a MnO₂/graphene composite modified electrode