Low molecular weight microtubule-associated proteins are light chains of microtubule-associated protein 1 (MAP 1).

Vallee, Richard B.; Davis, Susan E.

doi:10.1073/pnas.80.5.1342

Cited by 101 publications

(71 citation statements)

References 32 publications

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“…The Map1a gene encodes a precursor polypeptide that is proteolytically cleaved to produce a MAP1A heavy chain (MAP1A-HC) and a light chain (LC2; Langkopf et al, 1992). These proteins can bind to MTs independently or as a complex that can include LC1, a proteolytic cleavage product from MAP1B precursor protein (Hammarback et al, 1991), and LC3, an independently encoded autophagosomal protein (Vallee and Davis, 1983;Mann and Hammarback, 1994;Kabeya et al, 2000). In addition to binding with MT, MAP1A-HC interacts with the membrane-associated guanylate kinases (MAGUKs) through a C-terminal consensus domain (Brenman et al, 1998;Reese et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Mutations in the Microtubule-Associated Protein 1A (Map1a) Gene Cause Purkinje Cell Degeneration

2015

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The structural microtubule-associated proteins (MAPs) are critical for the organization of neuronal microtubules (MTs). Microtubuleassociated protein 1A (MAP1A) is one of the most abundantly expressed MAPs in the mammalian brain. However, its in vivo function remains largely unknown. Here we describe a spontaneous mouse mutation, nm2719, which causes tremors, ataxia, and loss of cerebellar Purkinje neurons in aged homozygous mice. The nm2719 mutation disrupts the Map1a gene. We show that targeted deletion of mouse Map1a gene leads to similar neurodegenerative defects. Before neuron death, Map1a mutant Purkinje cells exhibited abnormal focal swellings of dendritic shafts and disruptions in axon initial segment (AIS) morphology. Furthermore, the MT network was reduced in the somatodendritic and AIS compartments, and both the heavy and light chains of MAP1B, another brain-enriched MAP, was aberrantly distributed in the soma and dendrites of mutant Purkinje cells. MAP1A has been reported to bind to the membrane-associated guanylate kinase (MAGUK) scaffolding proteins, as well as to MTs. Indeed, PSD-93, the MAGUK specifically enriched in Purkinje cells, was reduced in Map1a Ϫ/ Ϫ Purkinje cells. These results demonstrate that MAP1A functions to maintain both the neuronal MT network and the level of PSD-93 in neurons of the mammalian brain.

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Section: Introductionmentioning

confidence: 99%

Mutations in the Microtubule-Associated Protein 1A (Map1a) Gene Cause Purkinje Cell Degeneration

2015

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“…MAPs(and tau) are considered to be indispensable for tubulin assembly into tubular structures. They have diameters of 3-9 nm and lengths of 20-40 nmand associate head to laterally with microtubules (27,28,32,59).…”

Section: Discussionmentioning

confidence: 99%

On the three-dimensional structure of quick-frozen hepatic mallory bodies with special reference to the appearance of cytoplasmic vesicles.

Irie

Koyama

Ikeuchi

et al. 1991

Cell Struct. Funct.

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ABSTRACT. Livers containing Mallory bodies (MBs, hyalin degenerative cytoplasmic inclusions) were examined using Heuser's and Van Harreveld's cryo-techniques. The tissues were collected from 1) a patient suffering from alcoholic hepatitis and 2) mice treated with griseofulvin (GF, an anti-mitotic drug). Normal mouse liver and isolated MBsfrom GF-treated mice were also analyzed by the same methods. Our results suggest that under the toxic influence of alcohol or GF on microtubular elements, MBsare generated by entanglement of elements of 10 nm filaments with microtubule elements. This in turn inhibits cellular transport processes. The reticular net of the ER-element which is usually observable in the normal tissue is changed into numerous small vesicles in the pathological and experimental tissues. The diameters of hepatocytes containing these vesicles were 1.5 to 2 times larger than control diameters. MBshave previously been described in thin sections as filamentous tangles. On replicas we found that they appear to be composed of pairs of filaments twisted in a roughly helical manner, each having a diameter less than 10 nm. The paired helical nature of the MB-filaments is reminiscent of other inclusion bodies, which are also composedof elements of 10 nmfilaments, observable in various neurological diseases.

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“…MAP1B is a major neuronal cytoskeleton protein that interacts with microtubules of the cellular cytoskeleton [25][26][27]; it is strongly expressed in the developing nervous system [28]. MAP1B is critically important in the localization of specific RNAs, in the maintenance of cell shape, and in other cellular functions.…”

Section: Alteration In Rpl15 and Map1b Expression In Bovine Folliculamentioning

confidence: 99%

Identification of Differentially Expressed Genes in Bovine Follicular Cystic Ovaries

Choe

Cho

Kim

et al. 2010

Korean J Physiol Pharmacol

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Follicular cystic ovary (FCO) is one of the most frequently diagnosed ovarian diseases and is a major cause of reproductive failure in mammalian species. However, the mechanism by which FCO is induced remains unclear. Genetic alterations which affect the functioning of many kinds of cells and/or tissues could be present in cystic ovaries. In this study, we performed a comparison analysis of gene expression in order to identify new molecules useful in discrimination of bovine FCO with follicular cystic follicles (FCFs). Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and ≥25 mm). These follicles had granulosa cell layer and theca interna and the hormone 17β-estradiol (E2)/ progesterone (P4) ratio in follicles was greater than one. Perifollicular regions including follicles were used for the preparation of RNA or protein. Differentially expressed genes (DEG) that showed greater than a 2-fold change in expression were screened by the annealing control primer (ACP)-based PCR method using GeneFishing TM DEG kits in bovine normal follicles and FCFs. We identified two DEGs in the FCFs: ribosomal protein L15 (RPL15) and microtubule-associated protein 1B (MAP1B) based on BLAST searches of the NCBI GenBank. Consistent with the ACP analysis, semi-quantitative PCR data and Western blot analyses revealed an up-regulation of RPL15 and a down-regulation of MAP1B in FCFs. These results suggest that RPL15 and MAP1B may be involved in the regulation of pathological processes in bovine FCOs and may help to establish a bovine gene data-base for the discrimination of FCOs from normal ovaries.

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Low molecular weight microtubule-associated proteins are light chains of microtubule-associated protein 1 (MAP 1).

Cited by 101 publications

References 32 publications

Mutations in the Microtubule-Associated Protein 1A (Map1a) Gene Cause Purkinje Cell Degeneration

Mutations in the Microtubule-Associated Protein 1A (Map1a) Gene Cause Purkinje Cell Degeneration

On the three-dimensional structure of quick-frozen hepatic mallory bodies with special reference to the appearance of cytoplasmic vesicles.

Identification of Differentially Expressed Genes in Bovine Follicular Cystic Ovaries

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