Low molecular weight heparin protection against oxalate-induced oxidative renal insult

A, Rajeswari; Varalakshmi, P.

doi:10.1016/j.cca.2006.01.027

Cited by 14 publications

(7 citation statements)

References 24 publications

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“…Sulfated polysaccharides are widespread in marine algae, especially brown seaweeds. Sulfated polysaccharides show various biological activities, including anticoagulant, antioxidant, antiviral, anticancer and immunomodulating activities [1,2,3,4,5]. Zhang et al [6] reported that sulfated polysaccharides play important roles as free-radical scavengers and antioxidants, which can prevent oxidative damage in living organisms.…”

Section: Introductionmentioning

confidence: 99%

“…Zhang et al [6] reported that sulfated polysaccharides play important roles as free-radical scavengers and antioxidants, which can prevent oxidative damage in living organisms. The low molecular weight polysaccharides heparin and fucoidan provided protection against oxalate (Ox)-induced oxidative renal injury [3,7]. Ox and calcium oxalate monohydrate (COM) induce the generation of free radicals, which are major mediators of the pathologic consequences of the formation of kidney stones [8].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Properties of Polysaccharide from the Brown Seaweed Sargassum graminifolium (Turn.), and Its Effects on Calcium Oxalate Crystallization

Zhang

Wang

et al. 2012

Marine Drugs

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Abstract:We investigated the effects of polysaccharides from the brown seaweed Sargassum graminifolium (Turn.) (SGP) on calcium oxalate crystallization, and determined its antioxidant activities. To examine the effects of SGP on calcium oxalate crystallization, we monitored nucleation and aggregation of calcium oxalate monohydrate crystals, using trisodium citrate as a positive control. We assessed antioxidant activities of SGP by determining its reducing power, its ability to scavenge superoxide radicals, and its activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The nucleation inhibition ratio of trisodium citrate and SGP was 58.5 and 69.2%, respectively, and crystal aggregation was inhibited by 71.4 and 76.8%, respectively. Increasing concentrations of SGP resulted in increased scavenging of superoxide anions and DPPH radicals (IC 50 = 1.9 and 0.6 mg/mL, respectively). These results suggest that SGP could be a candidate for treating urinary stones because of its ability to inhibit calcium oxalate crystallization and its antioxidant properties.Keywords: calcium oxalate crystallization; antioxidant; polysaccharide; Sargassum graminifolium OPEN ACCESSMar. Drugs 2012, 10 120 Abbreviations SGP, polysaccharide from Sargassum graminifolium; IR, infrared spectrum; DPPH, 1,1-diphenyl-2-picrylhydrazyl free radical; Ox, oxalate; CaOx, calcium oxalate; OD, optical density; S N , maximum increase of optical density with time; S A , rate of aggregation derived from the maximum decrease in optical density; t max , maximum time; COM, Calcium oxalate monohydrate; COD, Calcium oxalate dehydrate; COT, Calcium oxalate trihydrate.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant Properties of Polysaccharide from the Brown Seaweed Sargassum graminifolium (Turn.), and Its Effects on Calcium Oxalate Crystallization

Zhang

Wang

et al. 2012

Marine Drugs

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“…After the application of LMWH nebulization therapy, the SOD and GSH-Px activity was rebounded and the increase of MDA content was significantly inhibited, indicating that LMWH nebulization can play a role of antioxidant, reduce oxidative stress reaction, and attenuate the ALI. Rajeswari and Varalakshmi also found that LMWH has antioxidant potential in countering the oxidative challenge in the experimental oxidative renal injury model of rats [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Low Molecular Weight Heparin Nebulization Attenuates Acute Lung Injury

Xie

Huan

Tian

et al. 2017

BioMed Research International

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Background As acute lung injury (ALI) caused high mortality rate, it is important to explore the protection and treatment of ALI. The aim of the current study is to evaluate the effect of low molecular weight heparin (LMWH) nebulization on attenuating acute lung injury and the associated mechanism. Methods The arterial blood gas, total protein content in bronchoalveolar lavage fluid, lung wet/dry weight ratio, malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and Akt phosphorylation were evaluated after the ALI rabbits were treated with or without LMWH nebulization. Results PaO2 was increased and lung wet/dry weight ratio as well as total protein content in BALF was decreased after LMWH nebulization. After the application of LMWH nebulization therapy, the SOD and GSH-Px activity was rebounded and the increase of MDA content was significantly inhibited. The Akt protein phosphorylation level was decreased after LMWH nebulization therapy. Conclusions LMWH nebulization treatment can relieve the traumatic ALI in rabbits and inhibit oxidative stress possibly by suppressing the Akt phosphorylation.

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“…Numerous reports support the fact that fucoidan is functionally similar to heparin and its mode of action can be related with heparin (Pereira et al 1999;Shanmugam & Mody 2000). It has been suggested that exogenous GAG administration in the form of low-molecular-weight heparin improves the anionic charges on the tubular epithelium, which is depleted during calculogenesis and thereby decreases tubular uptake of crystals (Rajeswari & Varalakshmi 2006). This coating phenomenon principally defies calcium oxalate crystals and oxalate from gaining easy access to renal tubular epithelium by way of charge repulsion action.…”

Section: Group I Controlmentioning

confidence: 99%

Physico-chemical alterations of urine in experimental hyperoxaluria: a biochemical approach with fucoidan

Veena

Josephine

Preetha

et al. 2007

Journal of Pharmacy and Pharmacology

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Urinary supersaturation-induced crystal formation has been attributed as one of the key factor for the pathogenesis/progression of lithogenesis. This study was aimed at investigating whether fucoidan, a naturally occurring sulfated glycosaminoglycan, could ameliorate the biochemical changes in urine induced by stone formation. Two groups of male albino Wistar rats (120+/-20 g) received 0.75% ethylene glycol (EG) for 28 days to induce hyperoxaluria, and one of them received sulfated polysaccharides (fucoidan from Fucus vesiculosus, 5 mg kg(-1), s.c.), commencing from the 8(th) day of the experimental period. One group was maintained as normal control group and another group served as drug control, which received sulfated polysaccharides. The urine collected from all the groups was analysed for changes in pH, volume, oxalate, calcium, phosphorus, uric acid, magnesium, citric acid and glycosaminoglycans. Urinary crystals were analysed with a light microscope. Renal tissues were studied under polarized light for deposition of crystals and also analysed for their oxalate and calcium content. The changes in extracellular matrix on crystal deposition were also evaluated. The urinary pH and volume were altered in rats treated with EG along with an increase in weight of the kidney. Further, administration of EG to rats increased the supersaturation of urine by escalating the levels of the stone-forming constituents, such as oxalate, calcium, phosphorus and uric acid, which was completely restored by fucoidan treatment. The decrease in the inhibitors, like citrate, magnesium and glycosaminoglycans, in urine was prevented by the co-treatment with fucoidan. In hyperoxaluric rats, there was an increased excretion of calcium oxalate monohydrate crystals in urine along with crystal deposition in renal tissues; this was prevented by fucoidan treatment. Fucoidan administration reversed even the tissue levels of calcium and oxalate. The increased accumulation of collagen and expression of transforming growth factor-beta(1) in hyperoxaluria was normalized on fucoidan administration. These results suggest that the physico-chemical alterations in urine produced during hyperoxaluria can be reversed by fucoidan administration.

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Low molecular weight heparin protection against oxalate-induced oxidative renal insult

Cited by 14 publications

References 24 publications

Antioxidant Properties of Polysaccharide from the Brown Seaweed Sargassum graminifolium (Turn.), and Its Effects on Calcium Oxalate Crystallization

Antioxidant Properties of Polysaccharide from the Brown Seaweed Sargassum graminifolium (Turn.), and Its Effects on Calcium Oxalate Crystallization

Low Molecular Weight Heparin Nebulization Attenuates Acute Lung Injury

Physico-chemical alterations of urine in experimental hyperoxaluria: a biochemical approach with fucoidan

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